The Structure of a Biosynthetic Intermediate of Pyrroloquinoline Quinone (PQQ) and Elucidation of the Final Step of PQQ Biosynthesis

Journal of the American Chemical Society

Volumn 126, Issue 17, 2004, Pages 5342-5343

  Magnusson, Olafur T ^a   Toyama, Hirohide ^b   Saeki, Megumi ^b   Schwarzenbacher, Robert ^c   Klinman, Judith P ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b YAMAGUCHI UNIVERSITY   (Japan)

^c BURNHAM INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PYRROLOQUINOLINEQUINONE;

ARTICLE; BIOSYNTHESIS; DEHYDROGENATION; ELECTRON TRANSPORT; KLEBSIELLA PNEUMONIAE; METHYLOBACTERIUM; NONHUMAN; OXIDATION; PROTON NUCLEAR MAGNETIC RESONANCE; STRUCTURE ANALYSIS; ULTRAVIOLET SPECTROSCOPY;

MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR STRUCTURE; PQQ COFACTOR; SPECTROPHOTOMETRY;

EID: 2342652839     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0493852     Document Type: Article

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16. 3 buffer, pH 8.5 and incubated for 1 h at 40 °C before quenching with HCl as described.8b Both fluorescamine and TNBS assays yielded 0.4-0.6 mol amine per mol substrate based on tyrosine as a standard. The low yield can in part be explained by the instability of the substrate under mildly alkaline conditions. The substrate was shown to have a half-life of 135 min in 0.1 M Na-phosphate buffer, pH 8.0, at 25 °C in a pseudo-first-order reaction, by monitoring its disappearance by HPLC (data not shown).
17. 1H NMR spectrum along with complete assignments is available in the Supporting Information. All NMR experiments were performed on a Bruker DRX-500 instrument at 25 °C. Samples (60 μL) were in a 1.5-mm capillary that was kept nonspinning inside a 5-mm NMR tube.