Effect of single mismatches at 3′-end of primers on polymerase chain reaction

Sultan Qaboos University Medical Journal

Volumn 2, Issue 1, 2000, Pages 11-14

  Simsek, M ^a   Adnan, H ^a  

^a SULTAN QABOOS UNIVERSITY   (Oman)

Author keywords

Mismatched primers; PCR; globin gene

Indexed keywords

EID: 2342609348     PISSN: 2075051X     EISSN: 20750528     Source Type: Journal    
DOI: None     Document Type: Article

References (13)

1. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA. Primer-directed enzymatic amplification of DNA by a thermostable DNA polymerase. Science 1988, 239
2. Newton CR, Graham A., Enzyme Choice in PCR. Introduction to Biotechniques, Bios Scientific Publishers, 1994, pp: 12-21.
3. Gibbs RA, Nguyen PN, Caskey CT. Detection of single base differences by competitive oligonucleotide priming. Nucleic Acids Res 1989, 17
4. Eiken H.G, Odland E, Boman H, Skjelkvale L, Engebretsen LF, Apold J. Applications of natural and amplification created sites for the diagnosis of PKU mutations. Nucleic Acids Res 1991, 19, 427-30.
5. Tsai MY, Hanson NQ, Copeland KR, Beheshti I, Garg U. Determination of a T/G polymorphism at nucleotide 3206 of the apolipoprotein cIII gene by amplification refractory mutation system. Clin Chem 1994, 40, 2235-39.
6. Lindeman R, Hu SP, Volpato F, Trent RJ. PCR mutagenesis enabling rapid non-radioactive detection of common •-thalassemia mutations in Mediterraneans. Br J Haematol 1991, 78, 100-4.
7. Okayama H, Curiel DT, Brantly ML, Holmes MD, Crystal RG. Rapid, nonradioactive detection of mutations in the human genome by allele-specific amplification. J Clin Med 1989, 144, 105-13.
8. Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988
9. Saiki RK.,Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anaemia. Science 1985, 230, 1350-54.
10. Newton CR, Graham A, Heptinstall LE, Powell SJ, Summers C, Kalshekar N, Smith JC, Markham AF. Analysis of any point mutation in DNA; the amplification refractory mutation system (ARMS). Nucleic Acids Res 1989, 17, 2503-16.
11. Kwok S, Kellogg DE, McKinney N, Spasic D, Goda L, Levenson C, Sninsky JJ. Effects of primer-template mismatches on the polymerase chain reaction. Nucleic Acids Res, 1990, 18, 1999-1005
12. Wu-D Y, Ugozzoli L, Pal BK, Wallace RB. Allele specific enzymatic amplification of β-globin genomic DNA for diagnosis of sickle cell anaemia. Proc Natl Acad Sci USA 1989, 86, 2757-60.
13. Ehlen T, Dubeau L. Detection of ras point mutations by PCR using mutations-specific, inosine containing oligonucleotide primers. Biochem Biophys Res Commun 160, 441.