Improvement of automatic in-gel digestion by in situ alkylation of proteins

Journal of Biomolecular Techniques

Volumn 14, Issue 3, 2003, Pages 191-196

  Yokono, Takeshi ^a   Mineki, Reiko ^b   Taka, Hikari ^b   Kotaniguchi, Hiroto ^a   Murayama, Kimie ^b  

^a M and S Instruments Trading Inc   (Japan)

^b JUNTENDO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Automation; In situ alkylation; In gel digestion; Protein; Proteomics

Indexed keywords

PROTEIN; TRANSFERASE;

ALKYLATION; ANIMAL; ARTICLE; CATTLE; CHEMISTRY; MASS SPECTROMETRY; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ALKYLATION; ANIMALS; CATTLE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MASS SPECTROMETRY; PROTEINS; TRANSFERASES;

EID: 2342590873     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (4)

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2. Ashman K, Houthaeve T, Clayton J, et al. The application of robotics and mass spectrometry to the characterisation of the Drosophila melanogaster indirect flight muscle proteome. Lett Peptide Sci 1997;4:57-65
3. Mineki R, Taka H, Fujimura T, Kikkawa M, Shindo N, Murayama K. In situ alkylation for identification of cysteinyl residues in proteins during one- and two-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Proteomics 2002;2:1672-1681
4. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-685.