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Volumn 14, Issue 11, 2004, Pages 2883-2887

Synthesis of jaspaquinol and effect on viability of normal and malignant bladder epithelial cell lines

Author keywords

[No Author keywords available]

Indexed keywords

ANTINEOPLASTIC AGENT; AROMATIC COMPOUND; JASPAQUINOL; TERPENE DERIVATIVE; UNCLASSIFIED DRUG;

EID: 2342534553     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2004.03.055     Document Type: Article
Times cited : (15)

References (27)
  • 18
    • 0001396854 scopus 로고
    • Various Pd-complexes of geranyl chloride demonstrate that the distal olefin forms a stable chelated system (NMR and X-ray structure) see:
    • Various Pd-complexes of geranyl chloride demonstrate that the distal olefin forms a stable chelated system (NMR and X-ray structure) see: Akermark B., Hansson S., Krakenberger B., Vitagliano A., Zetterberg K. Organometallics. 3:1984;679
    • (1984) Organometallics , vol.3 , pp. 679
    • Akermark, B.1    Hansson, S.2    Krakenberger, B.3    Vitagliano, A.4    Zetterberg, K.5
  • 26
    • 0028152842 scopus 로고
    • Normal human urothelial (NHU) cell lines were established from surgical specimens taken from patients with no history of urothelial malignancy, as previously described, see:
    • Normal human urothelial (NHU) cell lines were established from surgical specimens taken from patients with no history of urothelial malignancy, as previously described, see: Southgate J., Hutton K.A., Thomas D.F., Trejdosiewicz L.K. Lab. Invest. 71:1974;583
    • (1974) Lab. Invest. , vol.71 , pp. 583
    • Southgate, J.1    Hutton, K.A.2    Thomas, D.F.3    Trejdosiewicz, L.K.4
  • 27
    • 0020328955 scopus 로고
    • 2 in air and were passaged by trypsinisation. The inhibitor, 1, was dissolved fresh in DMSO. The concentration of the DMSO carrier was kept constant at 0.1% and a solvent control was included in all experiments
    • 3 cells/well and the cells were grown for 16 h. The growth medium was aspirated, and 200 μL of medium containing the appropriate concentration of 1 was added to 96-well plates in replicates of 6. The cells were exposed to the agent for 24 or 72 h (day 1 and day 3 time points, respectively) at 37°C; the medium was replaced and the cells were cultured for a further 3 days (day 6 time point). Cell growth was assessed using an MTT ([3-(4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay on days 0, 1, 3, and 6 after the start of the treatment. 200 μL of 0.5 mg/mL (for NHU) or 0.25 mg/mL (for EJ) MTT (Sigma) was added to the wells of the plate, and plates were incubated at 37°C for 4 h. During this time, the yellow MTT tetrazolium salt was converted to insoluble, purple formazan crystals by mitochondrial dehydrogenase in viable cells. Nonconverted MTT and media were discarded, and the formazan crystals were dissolved in 200 μL DMSO. The absorbance was read at 570 nm relative to a DMSO blank. The data are displayed graphically as a mean value with error bars representing the standard deviation (SD). One way ANOVA was performed using GraphPad InStat to test for significant differences between the treatments and the controls. P values of 0.05 and below were considered to be significant
    • (1982) Nature , vol.297 , pp. 474
    • Parada, L.F.1    Tabin, C.J.2    Shih, C.3    Weinberg, R.A.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.