Biocompatibility of Trypan Blue with Human Corneal Cells

Archives of Ophthalmology

Volumn 122, Issue 5, 2004, Pages 736-742

  Van Dooren, Bart T H ^a   Beekhuis, W Houdijn ^a   Pels, Elisabeth ^b  

^a ROTTERDAM EYE HOSPITAL   (Netherlands)

^b NETHERLANDS INSTITUTE FOR NEUROSCIENCE   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; TRYPAN BLUE;

ARTICLE; BIOCOMPATIBILITY; CELL CULTURE; CELL DENSITY; CELL STRUCTURE; CELL SURVIVAL; CELL VIABILITY; COLORIMETRY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CORNEA CELL; CORNEA ENDOTHELIUM; CYTOTOXICITY; DONOR; FIBROBLAST; HUMAN; HUMAN CELL; IN VITRO STUDY; PRIORITY JOURNAL; SAFETY;

BIOCOMPATIBLE MATERIALS; CELL COUNT; CELL SURVIVAL; CELLS, CULTURED; COLORING AGENTS; CORNEAL STROMA; DOSE-RESPONSE RELATIONSHIP, DRUG; ENDOTHELIUM, CORNEAL; FIBROBLASTS; HUMANS; SAFETY; TIME FACTORS; TRYPAN BLUE;

EID: 2342533729     PISSN: 00039950     EISSN: None     Source Type: Journal    
DOI: 10.1001/archopht.122.5.736     Document Type: Article

References (23)

1. Stocker FW, King EH, Lucas DO, Georgiade N. A comparison of two different staining methods for evaluating corneal endothelial viability. Arch Ophthalmol. 1966;76:833-835.
2. Evans HM, Schulemann W. The action of vital stains belonging to the benzidine group. Science. 1914;39:443-453.
3. Pappenheimer AM. Experimental studies upon lymphocytes. J Exp Med. 1917;25:633-650.
4. Sperling SS. Evaluation of the endothelium of human donor corneas by induced dilation of intercellular spaces and trypan blue. Graefes Arch Clin Exp Ophthalmol. 1986;224:428-434.
5. Sperling SS, Olsen T, Ehlers N. Fresh and cultured corneal grafts compared by post-operative thickness and endothelial cell density. Acta Ophthalmol (Copenh). 1981;59:566-575.
6. Sperling SS. Cryopreservation of human cadaver corneas regenerated at 31°C in a modified tissue culture medium. Acta Ophthalmol (Copenh). 1981;59:142-148.
7. Sperling SS. Assessment of endothelial cell density in bovine corneas after osmotically induced dilation of intercellular spaces. Cornea. 1985;4:71-79.
8. Pels E, Schuchard Y. Organ-culture preservation of human corneas. Ooc Ophthalmol. 1983;56:147-153.
9. Pels E, Schuchard Y. The effects of high molecular weight dextran on the preservation of human corneas. Cornea. 1984-1985;3:219-227.
10. Norn MS. Vital staining of corneal endothelium in cataract extraction. Acta Ophthalmol (Copenh). 1971;49:725-733.
11. Norn MS. Pachometric study on the influence of corneal endothelial vital staining. Acta Ophthalmol (Copenh). 1973;51:679-686.
12. Norn MS. Per operative trypan blue vital staining of corneal endothelium: eight years' follow up. Acta Ophthalmol (Copenh). 1980;58:550-555.
13. Melles GRJ, de Waard PWT, Pameyer JH, Beekhuis WH. Trypan blue capsule staining to visualize the capsulorhexis in cataract surgery. J Cataract Refract Surg. 1999;25:7-9.
14. Pels E, Schuchard Y. Organ culture in the Netherlands: preservation and endothelial cell evaluation. In: Brightbill FS, ed. Corneal Surgery: Theory, Technique, and Tissue. 2nd ed. St Louis, Mo: CV Mosby Co; 1993:622-632.
15. Pels E, Beekhuis WH, Völker-Dieben HJ. Long-term tissue storage for keratoplasty. In: Brightbill FS, ed. Corneal Surgery: Theory, Technique, and Tissue. 3rd ed. St Louis, Mo: CV Mosby Co; 1998:897-906.
16. Fitzgerald PH, Rosman I. Sex chromosome loss and clones characterized by autosomal abnormality in cultured corneal fibroblast of man. Cytogenet Cell Genet. 1984;38:29-34.
17. Hoppenreijs VP, Pels E, Vrensen GF, Felten PC, Treffers WF. Platelet-derived growth factor: receptor expression in corneas and effects on corneal cells. Invest Ophthalmol Vis Sci. 1993;34:637-649.
18. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65:55-63.
19. Pels E, Nuyts RMA, Breebaart AC, Hartmann C. Rapid quantitative assays for corneal endothelial cell viability in vitro. Cornea. 1993;12:289-294.
20. Pels E, Vrensen GFJM. Microbial decontamination of human donor eys with povidone-iodine: penetration, toxicity, and effectiveness. Br J Ophthalmol. 1999;83:1019-1026.
21. Veckeneer M, van Overdam K, Monzer J, et al. Ocular toxicity study of trypan blue injected into the vitreous cavity of rabbit eyes. Graefes Arch Clin Exp Ophthalmol. 2001;239:698-704.
22. Stocker FW, King EH, Lucas DO, Georgiade NA. Clinical test for evaluating donor corneas. Arch Ophthalmol. 1970;84:2-7.
23. Van Dooren BTH, De Waard PW, Poort-van Nouhuys H, Beekhuis WH, Melles GRJ. Corneal endothelial cell density after trypan blue capsule staining in cataract surgery. J Cataract Refract Surg. 2002;28:574-575.