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Volumn 4, Issue 4, 2004, Pages 737-740

Isolation and characterization of trioxyethylene-encapsulated gold nanoclusters functionalized with a single DNA strand

Author keywords

[No Author keywords available]

Indexed keywords

ETHYLENE DERIVATIVE; GOLD; SINGLE STRANDED DNA;

EID: 2342527946     PISSN: 15306984     EISSN: None     Source Type: Journal    
DOI: 10.1021/nl0499591     Document Type: Article
Times cited : (22)

References (32)
  • 20
    • 2342653126 scopus 로고    scopus 로고
    • note
    • -1 (provided by the manufacturer).
  • 21
    • 2342539983 scopus 로고    scopus 로고
    • note
    • -1. The molecular weight estimate is based on the average core size of the nanoclusters as determined by TGA [Snow, unpublished results] using a previously published model of gold core atomic packing and thiol ligand surface coverage (ref 22).
  • 23
    • 2342631485 scopus 로고    scopus 로고
    • note
    • Agarose gels, 2%, were made in TBE (Tris-borate-EDTA buffer, Sigma) and run in 0.5x TBE on a horizontal gel electrophoresis apparatus. All samples were treated with 0.2 vol of 30% glycerol prior to loading. The samples were run on the gel under an applied potential of 100 V for 20 min or until the fluorescent DNA ran past the middle of the gel. The gels were viewed using a Kodak Image Station 440 digital camera.
  • 24
    • 2342619720 scopus 로고    scopus 로고
    • note
    • 2 showed a light reddish-brown color, and when viewed under a fluorescent light, the salt-containing aqueous phase indicated the presence of the fluorescein-labeled DNA.
  • 26
    • 2342573799 scopus 로고    scopus 로고
    • note
    • Attempts to perform fluorescence measurements as a characterization method for our DNA-GNCs were inconclusive because when the fluor is in such a close proximity to the nanoparticle, it is quenched. However, the intensity of signal for the DNA labeled with fluor, on its own, displayed only a nominal decrease upon the addition of 10-fold excess AuEO3.
  • 30
    • 2342531955 scopus 로고    scopus 로고
    • note
    • Following Sandstrom et al. (ref 14), an aliquot of reaction 2 was compared with a sample containing an equal amount of DNA in the absence of AuEO3, both run side-by-side on the same gel. The amount of unreacted DNA was determined by the relative fluorescence intensities of the bands corresponding to unreacted DNA.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.