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note
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-1 (provided by the manufacturer).
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21
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2342539983
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note
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-1. The molecular weight estimate is based on the average core size of the nanoclusters as determined by TGA [Snow, unpublished results] using a previously published model of gold core atomic packing and thiol ligand surface coverage (ref 22).
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22
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0032488526
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Hostetler, M. J.; Wingate, J. E.; Zhong, C. J.; Harris, J. E.; Vachet, R. W.; Clark, M. R.; Londono, J. D.; Green, S. J.; Stokes, J. J.; Wignall, G. D.; Glish, G. L.; Porter, M. D.; Evans, N. D.; Murray, R. W. Langmuir 1998, 14, 17-30.
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23
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2342631485
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note
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Agarose gels, 2%, were made in TBE (Tris-borate-EDTA buffer, Sigma) and run in 0.5x TBE on a horizontal gel electrophoresis apparatus. All samples were treated with 0.2 vol of 30% glycerol prior to loading. The samples were run on the gel under an applied potential of 100 V for 20 min or until the fluorescent DNA ran past the middle of the gel. The gels were viewed using a Kodak Image Station 440 digital camera.
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24
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2342619720
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note
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2 showed a light reddish-brown color, and when viewed under a fluorescent light, the salt-containing aqueous phase indicated the presence of the fluorescein-labeled DNA.
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25
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0034669474
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Viswanadham, G.7
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26
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2342573799
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note
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Attempts to perform fluorescence measurements as a characterization method for our DNA-GNCs were inconclusive because when the fluor is in such a close proximity to the nanoparticle, it is quenched. However, the intensity of signal for the DNA labeled with fluor, on its own, displayed only a nominal decrease upon the addition of 10-fold excess AuEO3.
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27
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0003748291
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2342531955
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note
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Following Sandstrom et al. (ref 14), an aliquot of reaction 2 was compared with a sample containing an equal amount of DNA in the absence of AuEO3, both run side-by-side on the same gel. The amount of unreacted DNA was determined by the relative fluorescence intensities of the bands corresponding to unreacted DNA.
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31
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0037130509
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