Comparison of the enzyme-hydrolyzed casein, guanidination, and isotope dilution methods for determining ileal endogenous protein flow in the growing rat and pig

Journal of Animal Science

Volumn 81, Issue 10, 2003, Pages 2525-2534

  Hodgkinson, S M ^a,c   Souffrant, W B ^b   Moughan, P J ^a  

^a MASSEY UNIVERSITY   (New Zealand)

^b LEIBNIZ INSTITUTE FOR FARM ANIMAL BIOLOGY FBN   (Germany)

^c UNIVERSIDAD AUSTRAL DE CHILE   (Chile)

Author keywords

Endogenous protein; Lysine; Nitrogen; Pigs; Rats

Indexed keywords

ANIMALIA; SUS SCROFA;

EID: 2342507761     PISSN: 00218812     EISSN: None     Source Type: Journal    
DOI: 10.2527/2003.81102525x     Document Type: Article

References (32)

1. Alpers, D. H. 1972. Protein synthesis in intestinal mucosa: The effect of route of administration of precursor amino acids. J. Clin. Invest. 51:167-173.
2. Boisen, S., and P. J. Moughan. 1996a. Different expressions of dietary protein and amino acid digestibility in pig feeds and their application in protein evaluation: A theoretical approach. Acta Agric. Scand. Sect. A 46:165-172.
3. Boisen, S., and P. J. Moughan. 1996b. Dietary influences on endogenous ileal protein and amino acid loss in the pig - A review. Acta Agric. Scand. Sect. A 46:154-164.
4. Butts, C. A., P. J. Moughan, and W. C. Smith. 1992. Composition of endogenous ileal digesta nitrogen from the rat - The use of distilled water for digesta collection. J. Sci. Food Agric. 59:415-417.
5. Costigan, P., and K. J. Ellis 1987. Analysis of faecal chromium derived from controlled release marker devices. N. Z. J. Technol. 3:89-92.
6. 15N-isotope dilution technique for determining the recovery of endogenous protein and amino acids in digesta collected from the distal ileum in pigs. J. Anim. Sci. 70:1848-1856.
7. 15N-isotope dilution technique. J. Anim. Sci. 68:409-418.
8. Donkoh, A., P. J. Moughan, and P. C. H. Morel. 1995. Comparison of methods to determine the endogenous amino acid flow at the terminal ileum of the growing rat. J. Sci. Food Agric. 67:359-366.
9. 15N recovery in pigs. Pages 64-66 in Proc. 6th Int. Symp. Digestive Physiology in Pigs. EAAP Publ. No. 80. EAAP, Germany [Au: Please give the city in Germany where this was published.].
10. Hagemeister, H., and H. Erbersdobler. 1985. Chemical labelling of dietary protein by transformation of lysine to homoarginine: A new technique to follow intestinal digestion and absorption. Proc. Nutr. Soc. 44:133A.
11. Hodgkinson, S. M., and P. J. Moughan. 2000. Amino acids - The collection of ileal digesta and characterisation of the endogenous component. Pages 105-124 in Feed Evaluation - Principles and Practice. Wageningen Pers, Wageningen, The Netherlands.
12. 15N-dilution technique in piglets fed on peas (Pisum sativum) and common beans (Phaseolus vulgaris). Br. J. Nutr. 68:101-110.
13. 15N-labelled dietary proteins for determining true ileal amino acid digestibilies is limited by their rapid recycling in the endogenous secretions of pigs. J. Nutr. 126:2188-2198.
14. 15N-labelled diets. Pages 49-54 in Nitrogen Flow in Pig Production and Environmental Consequences. EAAP Publ. No. 69. Pudoc Scientific Publishing, Wageningen, The Netherlands.
15. 15N-labelled diets or animals. Pages 21-24 in Proc. 6th Int. Symp. Digestive Physiology in Pigs. EAAP Publ. No. 80. EAAP, Bad Doberan, Germany.
16. 15N-isotope dilution technique for determining the recovery of endogenous protein in ileal digesta of pigs: Effect of the pattern of blood sampling, precursor pools, and isotope dilution technique. J. Anim. Sci. 75:159-169.
17. 15N-isotope dilution technique for determining the recovery of endogenous protein in ileal digesta of pigs: Effect of dilution in the precursor pool for endogenous nitrogen secretion. J. Anim. Sci. 75:148-158.
18. Moughan, P. J., and S. M. Rutherfurd. 1990. Endogenous flow of total lysine and other amino acids at the distal ileum of the protein-or peptide-fed rat: The chemical labelling of gelatine protein by transformation of lysine to homoarginine. J. Sci. Food Agric. 52:179-192.
19. Moughan, P. J., and G. Schuttert. 1991. Composition of nitrogen-containing fractions in digesta from the distal ileum of pigs fed a protein-free diet. J. Nutr. 121:1570-1574.
20. Moughan, P. J., P. J. Buttery, C. P. Essex, and J. B. Soar. 1992. Evaluation of the isotope dilution technique for determining ileal endogenous nitrogen excretion in the rat. J. Sci. Food Agric. 58:165-172.
21. Moughan, P. J., A. J. Darragh, W. C. Smith, and C. A. Butts. 1990. Perchloric and trichloroacetic acids as precipitants of protein in endogenous ileal digesta from the rat. J. Sci. Food Agric. 52:13-21.
22. Reeds, P. J., D. G. Burrin, T. A. Davis, B. Stoll, L. J. Wykes, D. Wray-Cahen, F. Jahoor, M. A. Dudley, M. L. Fiorotto, and N. J. Gannon. 1997. A review - Growth, development and nutrient metabolism in piglets and infants. Pages 1-32 in Manipulating Pig Production VI. Australasian Pig Sci. Assoc., Werribee, Victoria, Australia.
23. 15N as compared with homoarginine suggests a lower prececal digestibility of casein in pigs. J. Nutr. 124:2404-2409.
24. Rutherfurd, S. M., and P. J. Moughan. 1990. Guanidination of lysine in selected dietary proteins. J. Agric. Food Chem. 38:209-211.
25. Schmitz, M., H. Hagemeister, and H. F. Erbersdobler. 1991. Homoarginine labelling is suitable for determination of protein absorption in miniature pigs. J. Nutr. 121:1575-1580.
26. 15N-isotope dilution method for determining ileal endogenous nitrogen excretion in the young (10 kg liveweight) pig. J. Sci. Food Agric. 69:41-50.
27. Siriwan P., W. L. Bryden, and E. F. Annison. 1994. Use of guanidinated dietary protein to measure losses of endogenous amino acids in poultry. Br. J. Nutr. 71:515-529.
28. Snedecor, G. W., and W. G. Cochran. 1980. Statistical Methods. 7th ed. Iowa State Univ. Press, Ames.
29. Souffrant, W. B., C. Février, J. P. Laplace, and U. Hennig. 1997. Comparison of methods to estimate ileal endogenous nitrogen and amino acids in piglets. Page 591-595 in Proc. 7th Int. Symp. Digestive Physiology in Pigs. EAAP Publ. No. 88. INRA, Saint Malo, France.
30. Souffrant, W. B., A. Rérat, J. P. Laplace, B. Darcy-Vrillon, R. Köhler, T. Corring, and G. Gebhardt. 1993. Exogenous and endogenous contributions to nitrogen fluxes in the digestive tract of pigs fed a casein diet. III. Recycling of endogenous protein. Reprod. Nutr. Dev. 33:373-382.
31. Tamminga, S., H. Schulz, J. van Bruchem, and J. Huisman. 1995. The nutritional significance of endogenous N-losses along the gastro-intestinal tract of farm animals. Arch. Anim. Nutr. 48:9-22.
32. Yu, Feng, P. J. Moughan, and T. N. Barry. 1995. Effect of condensed tannin in cottonseed hulls on endogenous ileal amino acid loss in the growing rat. J. Sci. Food Agric. 68:451-455.