Improvement of gene expression analysis by RQ-PCR technology: Addition of BSA [2]

Leukemia

Volumn 18, Issue 5, 2004, Pages 1022-1025

  Silvy, M ^a   Pic, G ^a   Gabert, J ^a   Picard, C ^a  

^a HÔPITAL NORD   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BCR ABL PROTEIN; BOVINE SERUM ALBUMIN; COMPLEMENTARY DNA; ONCOPROTEIN; TEL AML1 FUSION PROTEIN; TEL-AML1 FUSION PROTEIN; TRANSCRIPTION FACTOR RUNX1;

ACUTE LYMPHOBLASTIC LEUKEMIA; BLOOD SAMPLING; CONTROLLED STUDY; DNA PURIFICATION; FOLLOW UP; GENE AMPLIFICATION; GENE EXPRESSION; HUMAN; LETTER; MINIMAL RESIDUAL DISEASE; PRIORITY JOURNAL; QUANTITATIVE DIAGNOSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA DEGRADATION; RNA EXTRACTION; RNA ISOLATION; GENETICS; METHODOLOGY; ONCOGENE; POLYMERASE CHAIN REACTION;

CORE BINDING FACTOR ALPHA 2 SUBUNIT; GENE EXPRESSION; GENES, ABL; HUMANS; ONCOGENE PROTEINS, FUSION; POLYMERASE CHAIN REACTION; SERUM ALBUMIN, BOVINE;

EID: 2342467330     PISSN: 08876924     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.leu.2403339     Document Type: Letter

References (6)

1. de Haas V, Breunis WB, Dee R, Verhagen OJ, Kroes W, van Wering ER et al. The TEL-AML1 real-time quantitative polymerase chain reaction (PCR) might replace the antigen receptor-based genomic PCR in clinical minimal residual disease studies in children with acute lymphoblastic leukaemia. Br J Haematol 2002; 116: 87-93.
2. Moppett J, van der Velden VH, Wijkhuijs AJ, Hancock J, van Dongen JJ, Goulden N. Inhibition affecting RQ-PCR-based assessment of minimal residual disease in acute lymphohlastic leukemia: reversal by addition of bovine serum albumin. Leukemia 2003; 17: 268-270.
3. Abu Al-Soud W, Radstrom P. Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat. J Clin Microbiol 2000; 38: 4463-4470.
4. Abu Al-Soud W, Radstrom P. Capacity of nine thermostable DNA polymerases to mediate DNA amplification in the presence of PCR-inhibiting samples. Appl Environ Microbiol 1998; 64: 3748-3753.
5. Beillard E, Pallisgaard N, van der Velden VHJ, Bi W, Dee R, van der Schoot E et al. Evaluation of candidate control genes for diagnosis and MRD detection in leukemic patients using 'real-time' quantitative reverse-transcriptase polymerase chain reaction (RQ-PCR) - A Europe Against Cancer Program. Leukemia 2003; 17: 2474-2486.
6. Gabert J, Beillard E, van der Velden VHJ, Pallisgaard N, Gottardi E, Cazzaniga G et al. European standardization and quality control program of real time quantitative RT-PCR analysis of fusion gene transcripts for detection of minimal residual disease in leukemia patients. Leukemia 2003; 17: 2318-2357.