Development and Validation of an ELISA for Hemoglobin-A2: A Novel Method for β-Thalassemia Screening in Developing Countries

Journal of Immunoassay and Immunochemistry

Volumn 25, Issue 2, 2004, Pages 135-146

  Menon, Shyla R ^a   Khatkhatay, M Ikram ^a   Dandekar, Sucheta P ^b   Patel, Zarine M ^a  

^a NATIONAL INSTITUTE FOR RESEARCH IN REPRODUCTIVE HEALTH   (India)

^b KEM HOSPITAL   (India)

Author keywords

Thalassemia; ELISA; Hemoglobin A2; Hemolysates

Indexed keywords

HEMOGLOBIN A2; HORSERADISH PEROXIDASE; IMMUNOGLOBULIN;

ACCURACY; ARTICLE; BETA THALASSEMIA; ENZYME LINKED IMMUNOSORBENT ASSAY; HEMOGLOBIN DETERMINATION; HEMOLYSATE; HUMAN; PRIORITY JOURNAL; SCREENING TEST; VALIDATION PROCESS;

ANIMALS; BETA-THALASSEMIA; DEVELOPING COUNTRIES; ENZYME-LINKED IMMUNOSORBENT ASSAY; ERYTHROCYTES; HEMOGLOBIN A; HEMOGLOBIN A2; HUMANS; MASS SCREENING; RABBITS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 2342462983     PISSN: 15321819     EISSN: None     Source Type: Journal    
DOI: 10.1081/IAS-120030523     Document Type: Article

References (15)

1. Angastiniotis, M.; Englezoesp, M.B.; Boulyjenkov, V. Prevention and control of hemoglobinopathies. Bull. WHO 1995, 73, 375-396.
2. Varawalla, N.Y.; Old, J.M.; Venkateshan, S.R.; Weatherall, D.J. The spectrum of beta thalassemia mutations on the Indian subcontinent the basis of prenatal diagnosis. Brit. J. Hematol. 1991, 78, 242-247.
3. Sangani, B.; Sukumaran, P.K.; Mahadik, C.; Yagnik, H.; Telang, S.; Vas, F.; Oberoi, R.A.; Modell, B.; Merchant, S.M. Thalassemia in Bombay: the role of medical genetics in developing countries. Bull. WHO 1990, 68 (1), 75-81.
4. Sukumaran, P.K.; Randelia, H.P.; Merchant, S.M. Thalassemia syndromes in Bombay. J. Ind. Med. Sci. 1964, 11, 244.
5. Mahadik, C.; Kapadia, C.; Yagnik, H.; Sukumaran, P.K.; Merchant, S.M. One tube osmotic fragility as an useful screening test for thalassemia carriers-a field experience. Ind. J. Hematol. 1986, 4, 62-65.
6. 2. Clin. Chem. Acta 1974, 50, 413-418.
7. Angastiniotis, M.A.; Hadjiminas, M.G. Prevention of thalassemia in Cyprus. Lancet 1981, 1 (8216), 369-371.
8. Grenet, H.E.; Graver, F.A. Identification and quantitation of sickle cell hemoglobin with an enzyme linked immunosorbent assay (ELISA). J. Lab. Clin. Med. 1980, 96 (4), 597-605.
9. Graver, A.; Talmage, D.W. Comparative immunochemical studies of primate hemoglobins. Biochem. Genet. 1975, 13, 743-757.
10. Huisman, T.H.J.; Dozy, A.M. Studies on the heterogeneity of Hemoglobin IX. J. Chromatogr. 1965, 19, 160-169.
11. Avrameas, X.; Temynck, T. The cross-linking of proteins with gluteraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry 1969, 6, 53.
12. Johnston, A.; Thorpe, X. Purification of immunoglobulins, constituent chains and fragments. Immunochem. Pract. 1987, 48-85.
13. Hermanson, G.T. Antibody modification and conjugation. Bioconj. Techn. 1996, 460-492.
14. Engvall, E.; Perlmann, P. Enzyme linked immunosorbent assay, quantitative assay of immunoglobulins. Immunochem. 1971, 8, 871-874.
15. International committee for standardization in hematology. Simple electrophoretic system for presumptive identification of abnormal hemoglobins. Blood 1978, 52, 1965.