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note
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1-ATPase, ATP hydrolysis and reversible synthesis are at fast equilibrium. O18 exchange studies confirmed that ATP and ADP.Pi interconvert rapidly in the active site [1, 13]. To date the number of nucleotides and, in particular, the conformations bounded with ATP or ADP.Pi are not dynamically distinguishable [14, 34], unless Pi or ADP is further released from the active site.
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35
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