Comparison of TaqMan™ and Epoch Dark Quenchers™ during real-time reverse transcription PCR

Molecular and Cellular Probes

Volumn 18, Issue 3, 2004, Pages 207-209

  Daum, Luke T ^a,b   Ye, Keying ^c   Chambers, James P ^b   Santiago, Jose ^b   Hickman, John R ^a   Barnes, William J ^d   Kruzelock, Russell P ^d   Atchley, Daniel H ^a  

^a Air Force Materiel Command   (United States)

^b UNIVERSITY OF TEXAS AT SAN ANTONIO   (United States)

^c VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

^d Lackland Air Force Base ^*  (United States)

Author keywords

Dark Quenchers; Real time fluorogenic PCR; TaqMan PCR

Indexed keywords

FLUOROCHROME; FLUORESCENT DYE; TAQ POLYMERASE; VIRUS RNA;

ARTICLE; COMPARATIVE STUDY; FLUORESCENCE; MOLECULAR PROBE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; GENETICS; INFLUENZA VIRUS A; INSTRUMENTATION; METABOLISM; METHODOLOGY; SENSITIVITY AND SPECIFICITY;

FLUORESCENT DYES; INFLUENZA A VIRUS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; TAQ POLYMERASE;

EID: 2342447231     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2004.01.005     Document Type: Article

References (5)

1. Heid C.A., Stevens J., Livak K.J., Williams P.M. Real time quantitative PCR. Genome Res. 6:(10):1996;986-994
2. Epoch Biosystems Website. Available at: http://epochbio.com/products/ Dyes%20Quenchers_Dark.htm.
3. Lukhtanov E.A., Metcalf M., Reed M.W. Fluorogenic DNA probes for multicolor hybridization assays. 2001;American Biotechnology Laboratory
4. Afonina I.A., Reed M.W., Lusby E., Shishkina I.G., Belousov Y.S. Minor groove binder-conjugated DNA probes for quantitative DNA detection by hybridization-triggered fluorescence. Biotechniques. 32:(4):2002;940-944. see also 946-9
5. Amersham Biosciences. MGB Eclipse Probe Systems. Microsoft PowerPoint. Available at: www.amershambiosciences.com.