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The amplitude of the sensory responses in the second set of experiments with whole-cell recordings was larger than that in the first set of experiments because these neurons were located specifically in the C2 (or E2) columns, whereas in the first set of experiments the neurons were targeted through stereotaxic coordinates to a less-defined B/C-row region of barrel cortex.
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2342433920
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Materials and methods are available as supporting material on Science Online.
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2342446393
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In control animals, 38 connections were found from D- to C-row pyramidal neurons with a mean unitary EPSP amplitude of 155 μV. Originating from the same presynaptic D-row pyramidal neurons, 27 connections were found onto the E-row pyramidal neurons with a mean unitary EPSP amplitude of 121 μV. In deprived animals, 35 connections were found from D- to C-row pyramidal neurons with a mean unitary EPSP amplitude of 108 μV and 46 connections from D- and E-row pyramidal neurons with a mean unitary EPSP amplitude of 101 μV.
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2342429928
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The asymmetry index was calculated as (2 × (length of axon in E row)/[(length of axon in C row) + (length of axon in E row)]} - 1. We furthermore also measured this asymmetry index in neurons recorded in vitro, finding consistent results with our in vivo measurements. In control animals in vitro, the axonal asymmetry index was -0.08 ± 0.12 (n = 8), whereas in deprived animals in vitro, the asymmetry index was 0.24 ± 0.18 (n = 8).
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2342441875
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From eight in vivo control cells with soma in D row. E-row axon has 0. 203 ± 0.008 boutons/μm and C-row axon has 0.205 ± 0.006 boutons/μm. From eight in vivo deprived cells with somata in D row: E-row axon has 0.222 ± 0.006 boutons/μm and C-row axon has 0.213 ± 0.008 boutons/μm. These measurements from in vivo filled cells are not significantly different (P > 0.1, Student's t test). We also quantified our in vitro reconstructions. From eight in vitro control cells with soma in D row. E-row axon has 0.205 ± 0.009 boutons/μm and C-row axon has 0.198 ± 0.011 boutons/μm. From eight in vitro deprived cells with soma in D row: E-row axon has 0219 ± 0.018 boutons/μm and C-row axon has 0.199 ± 0.013 boutons/μm. These measurements from in vitro filled cells are not significantly different (P > 0.1, Student's t test).
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2342444591
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Axonal branch points of D-row pyramidal neurons reconstructed following in vivo recordings were quantified within the C row and the E row. In control animals, D-row pyramids (n = 8) had 4.6 ± 1.5 nodes in the C row and 2.3 ± 0.6 nodes in the E row. In deprived animals, D-row pyramids (n = 8) had 2.5 ± 0.8 nodes in the C row and 10 ± 2.4 nodes in the E row.
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We are grateful to D. Feldman, D. Simons, and R. Aronoff for helpful comments on an earlier version of this manuscript. We also thank D. Feldmeyer, F. Helmchen, M. Larkum, H. Spors, and J. Waters for useful discussions and technical advice. We are grateful to A. Grinvald for the gift of voltage-sensitive dye RH1691. Thanks are also due to M. Kaiser for staining of slices and S. Wiegert for reconstructing the morphology of the neurons.
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