Production and characterization of monoclonal antibodies against Enterocytozoon bieneusi purified from rhesus macaques

Infection and Immunity

Volumn 73, Issue 8, 2005, Pages 5166-5172

  Zhang, Quanshun ^a   Singh, Inderpal ^a   Sheoran, Abhineet ^a   Feng, Xiaochuan ^a   Nunnari, John ^a   Carville, Angela ^b   Tzipori, Saul ^a  

^a TUFTS UNIVERSITY   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EPITOPE; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN G2A; IMMUNOGLOBULIN G2B; MONOCLONAL ANTIBODY;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY PRODUCTION; ARTICLE; CROSS REACTION; ENTEROCYTOZOON; ENZYME LINKED IMMUNOSORBENT ASSAY; FECES ANALYSIS; HOST PARASITE INTERACTION; HUMAN; MICROSPORIDIOSIS; NONHUMAN; PRIORITY JOURNAL; RHESUS MONKEY; TRANSMISSION ELECTRON MICROSCOPY; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, MONOCLONAL; BLOTTING, WESTERN; ENTEROCYTOZOON; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; IMMUNOHISTOCHEMISTRY; MACACA MULATTA; MICE; MICROSCOPY, ELECTRON, TRANSMISSION; MICROSCOPY, IMMUNOELECTRON; MICROSPORIDIOSIS; SPORES, PROTOZOAN;

EID: 23344451061     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.73.8.5166-5172.2005     Document Type: Article

References (41)

1. Accoceberry, I., M. Thellier, A. Datry, I. Desportes-Livage, S. Biligui, M. Danis, and X. Santarelli. 2001. One-step purification of Enterocytozoon bieneusi spores from human stools by immunoaffinity expanded-bed adsorption. J. Clin. Microbiol. 39:1947-1951.
2. Accoceberry, I., M. Thellier, I. Desportes-Livage, A. Achbarou, S. Biligui, M. Danis, and A. Datry. 1999. Production of monoclonal antibodies directed against the microsporidium Enterocytozoon bieneusi. J. Clin. Microbiol. 37: 4107-4112.
3. Albrecht, H., and I. Sobottka. 1997. Enterocytozoon bieneusi infection in patients who are not infected with human immunodeficiency virus. Clin. Infect. Dis. 25:344.
4. Canning, E. U., and W. S. Hollister. 1990. Enterocytozoon bieneusi (Microspora): prevalence and pathogenicity in AIDS patients. Trans. R. Soc. Trop. Med. Hyg. 84:181-186.
5. Carville, A., K. Mansfield, G. Widmer, A. Lackner, D. Kotler, P. Wiest, T. Gumbo, S. Sarbah, and S. Tzipori. 1997. Development and application of genetic probes for detection of Enterocytozoon bieneusi in formalin-fixed stools and in intestinal biopsy specimens from infected patients. Clin. Diagn. Lab. Immunol. 4:405-408.
6. Chalifoux, L. V., A. Carville, D. Pauley, B. Thompson, A. A. Lackner, and K. G. Mansfield. 2000. Enterocytozoon bieneusi as a cause of proliferative serositis in simian immunodeficiency virus-infected immunodeficient macaques (Macaca mulatta). Arch. Pathol. Lab. Med. 124:1480-1484.
7. Chalifoux, L. V., J. MacKey, A. Carville, D. Shvetz, K. C. Lin, A. Lackner, and K. G. Mansfield. 1998. Ultrastructural morphology of Enterocytozoon bieneusi in biliary epithelium of rhesus macaques (Macaca mulatta). Vet. Pathol. 35:292-296.
8. Challier, S., S. Brown, C. Ombrouck, I. Desportes-Livage, D. De Nay, and M. Gentilini. 1994. Flow cytometry as a possible method of isolation of spores of the microsporidian Enterocytozoon bieneusi. J. Eukaryot. Microbiol. 41: 27S.
9. Davis, J. M., J. E. Pennington, A. M. Kubler, and J. F. Conscience. 1982. A simple, single-step technique for selecting and cloning hybridomas for the production of monoclonal antibodies. J. Immunol. Methods 50:161-171.
10. Desportes, I., Y. Le Charpentier, A. Galian, F. Bernard, B. Cochand-Priollet, A. Lavergne, P. Ravisse, and R. Modigliani. 1985. Occurrence of a new microsporidan: Enterocytozoon bieneusi n.g., n. sp., in the enterocytes of a human patient with AIDS. J. Protozool. 32:250-254.
11. French, A. L., L. M. Beaudet, D. A. Benator, C. S. Levy, M. Kass, and J. M. Orenstein. 1995. Cholecystectomy in patients with AIDS: clinicopathologic correlations in 107 cases. Clin. Infect. Dis. 21:852-858.
12. Gainzarain, J. C., A. Canut, M. Lozano, A. Labora, F. Carreras, S. Fenoy, R. Navajas, N. J. Pieniazek, A. J. da Silva, and C. del Aguila. 1998. Detection of Enterocytozoon bieneusi in two human immunodeficiency virus-negative patients with chronic diarrhea by polymerase chain reaction in duodenal biopsy specimens and review. Clin. Infect. Dis. 27:394-398.
13. Galfre, G., and C. Milstein. 1981. Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol. 73:3-46.
14. Gefter, M. L., D. H. Margulies, and M. D. Scharff. 1977. A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells. Somatic Cell Genet. 3:231-236.
15. Goetz, M., S. Eichenlaub, G. R. Pape, and R. M. Hoffmann. 2001. Chronic diarrhea as a result of intestinal microsporidiosis in a liver transplant recipient. Transplantation 71:334-337.
16. Graham, J. M. 2002. OptiPrep density gradient solutions for mammalian organelles. ScientificWorldJournal 2:1440-1443.
17. Green, L. C., P. J. Didier, L. C. Bowers, and E. S. Didier. 2004. Natural and experimental infection of immunocompromised rhesus macaques (Macaca mulatta) with the microsporidian Enterocytozoon bieneusi genotype D. Microbes Infect. 6:996-1002.
18. Guerard, A., M. Rabodonirina, L. Cotte, O. Liguory, M. A. Piens, S. Daoud, S. Picot, and J. L. Touraine. 1999. Intestinal microsporidiosis occurring in two renal transplant recipients treated with mycophenolate mofetil. Transplantation 68:699-707.
19. Gumbo, T., I. T. Gangaidzo, S. Sarbah, A. Carville, S. Tzipori, and P. M. Wiest. 2000. Enterocytozoon bieneusi infection in patients without evidence of immunosuppression: two cases from Zimbabwe found to have positive stools by PCR. Ann. Trop. Med. Parasitol. 94:699-702.
20. Kucerova, Z., H. Moura, G. J. Leitch, R. Sriram, C. Bern, V. Kawai, D. Vargas, R. H. Gilman, E. Ticona, A. Vivar, and G. S. Visvesvara. 2004. Purification of Enterocytozoon bieneusi spores from stool specimens by gradient and cell sorting techniques. J. Clin. Microbiol. 42:3256-3261.
21. Lopez-Velez, R., M. C. Turrientes, C. Garron, P. Montilla, R. Navajas, S. Fenoy, and C. del Aguila. 1999. Microsporidiosis in travelers with diarrhea from the tropics. J. Travel Med. 6:223-227.
22. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
23. Mansfield, K. G., A. Carville, D. Hebert, L. Chalifoux, D. Shvetz, K. C. Lin, S. Tzipori, and A. A. Lackner. 1998. Localization of persistent Enterocytozoon bieneusi infection in normal rhesus macaques (Macaca mulatta) to the hepatobiliary tree. J. Clin. Microbiol. 36:2336-2338.
24. Mansfield, K. G., A. Carville, D. Shvetz, J. MacKey, S. Tzipori, and A. A. Lackner. 1997. Identification of an Enterocytozoon bieneusi-like microsporidian parasite in simian-immunodeficiency-virus-inoculated macaques with hepatobiliary disease. Am. J. Pathol. 150:1395-1405.
25. McWhinney, P. H., D. Nathwani, S. T. Green, J. F. Boyd, and J. A. Forrest. 1991. Microsporidiosis detected in association with AIDS-related sclerosing cholangitis. AIDS 5:1394-1395.
26. Metge, S., J. T. Van Nhieu, D. Dahmane, P. Grimbert, F. Foulet, C. Sarfati, and S. Bretagne. 2000. A case of Enterocytozoon bieneusi infection in an HIV-negative renal transplant recipient. Eur. J. Clin. Microbiol. Infect. Dis. 19:221-223.
27. Mollenhauer, H. H. 1964. Plastic embedding mixtures for use in electron microscopy. Stain Technol. 39:111-114.
28. Pol, S., C. Romana, S. Richard, F. Carnot, J. L. Dumont, H. Bouche, G. Pialoux, M. Stern, J. F. Pays, and P. Berthelot. 1992. Enterocytozoon bieneusi infection in acquired immunodeficiency syndrome-related sclerosing cholangitis. Gastroenterology 102:1778-1781.
29. Pol, S., C. A. Romana, S. Richard, P. Amouyal, I. Desportes-Livage, F. Carnot, J. F. Pays, and P. Berthelot. 1993. Microsporidia infection in patients with the human immunodeficiency virus and unexplained cholangitis. N. Engl. J. Med. 328:95-99.
30. Rabodonirina, M., M. Bertocchi, I. Desportes-Livage, L. Cotte, H. Levrey, M. A. Piens, G. Monneret, M. Celard, J. F. Mornex, and M. Mojon. 1996. Enterocytozoon bieneusi as a cause of chronic diarrhea in a heart-lung transplant recipient who was seronegative for human immunodeficiency virus. Clin. Infect. Dis. 23:114-117.
31. Sandfort, J., A. Hannemann, H. Gelderblom, K. Stark, R. L. Owen, and B. Ruf. 1994. Enterocytozoon bieneusi infection in an immunocompetent patient who had acute diarrhea and who was not infected with the human immunodeficiency virus. Clin. Infect. Dis. 19:514-516.
32. Schwartz, D. A., D. C. Anderson, S. A. Klumpp, and H. M. McClure. 1998. Ultrastructure of atypical (teratoid) sporogonial stages of Enterocytozoon bieneusi (Microsporidia) in naturally infected rhesus monkeys (Macacca mulatta). Arch. Pathol. Lab. Med. 122:423-429.
33. Sheoran, A. S., X. Feng, S. Kitaka, L. Green, C. Pearson, E. S. Didier, S. Chapman, J. K. Tumwine, and S. Tzipori. 2005. Purification of Enterocytozoon bieneusi from stools and production of specific antibodies. J. Clin. Microbiol. 43:387-392.
34. Sing, A., K. Tybus, J. Heesemann, and A. Mathis. 2001. Molecular diagnosis of an Enterocytozoon bieneusi human genotype C infection in a moderately immunosuppressed human immunodeficiency virus-seronegative liver-transplant recipient with severe chronic diarrhea. J. Clin. Microbiol. 39:2371-2372.
35. Sobottka, I., H. Albrecht, J. Schottelius, C. Schmetz, M. Bentfeld, R. Laufs, and D. A. Schwartz. 1995. Self-limited traveller's diarrhea due to a dual infection with Enterocytozoon bieneusi and Cryptosporidium parvum in an immunocompetent HIV-negative child. Eur. J. Clin. Microbiol. Infect. Dis. 14:919-920.
36. Tzipori, S., A. Carville, G. Widmer, D. Kotler, K. Mansfield, and A. Lackner. 1997. Transmission and establishment of a persistent infection of Enterocytozoon bieneusi, derived from a human with AIDS, in simian immunodeficiency virus-infected rhesus monkeys. J. Infect. Dis. 175:1016-1020.
37. Van Veldhoven, P. P., E. Baumgart, and G. P. Mannaerts. 1996. Iodixanol (Optiprep), an improved density gradient medium for the iso-osmotic isolation of rat liver peroxisomes. Anal. Biochem. 237:17-23.
38. Visvesvara, G., G. J. Leitch, N. J. Pieniazek, A. J. Da Silva, S. Wallace, S. B. Slemenda, R. Weber, D. A. Schwartz, L. Gorelkin, C. M. Wilcox, et al. 1995. Short-term in vitro culture and molecular analysis of the microsporidian Enterocytozoon bieneusi. J. Eukaryot. Microbiol. 42:506-510.
39. Weber, R., H. Kuster, R. Keller, T. Bachi, M. A. Spycher, J. Briner, E. Russi, and R. Luthy. 1992. Pulmonary and intestinal microsporidiosis in a patient with the acquired immunodeficiency syndrome. Am. Rev. Respir. Dis. 146: 1603-1605.
40. Zhu, X., M. Wittner, H. B. Tanowitz, A. Cali, and L. M. Weiss. 1994. Ribosomal RNA sequences of Enterocytozoon bieneusi, Septata intestinalis and Ameson michaelis: phylogenetic construction and structural correspondence. J. Eukaryot. Microbiol. 41:204-209.
41. Zhu, X., M. Wittner, H. B. Tanowitz, D. Kotler, A. Cali, and L. M. Weiss. 1993. Small subunit rRNA sequence of Enterocytozoon bieneusi and its potential diagnostic role with use of the polymerase chain reaction. J. Infect. Dis. 168:1570-1575.