An in situ perfusion protocol of rat epididymal adipose tissue useful in metabolic studies

Journal of Lipid Research

Volumn 46, Issue 8, 2005, Pages 1803-1808

  Coǹsol, Gemma ^a   Moles, Anna ^a   Ricart Jané, David ^a   Llobera, Miquel ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

Author keywords

Adiponectin; Heparin; Lipolysis; Lipoprotein lipase; Release

Indexed keywords

ADENOSINE TRIPHOSPHATE; ADIPONECTIN; HEPARIN; LIPOPROTEIN LIPASE;

ADIPOSE TISSUE; ANIMAL TISSUE; ARTICLE; ENDOTHELIUM; ENZYME ACTIVITY; EPIDIDYMIS; HO AND MENG TECHNIQUE; IN SITU PERFUSION PROTOCOL; ISOLATED ORGAN; LIPOLYSIS; MALE; METABOLISM; MOUSE; NONHUMAN; PRIORITY JOURNAL; REPRODUCIBILITY; TECHNIQUE; WHITE ADIPOSE TISSUE;

ADENOSINE TRIPHOSPHATE; ADIPOSE TISSUE; ANIMALS; EPIDIDYMIS; LIPOLYSIS; LIPOPROTEIN LIPASE; MALE; METABOLISM; PERFUSION; RATS; RATS, WISTAR; RESEARCH;

EID: 23244458457     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: 10.1194/jlr.D500016-JLR200     Document Type: Article

References (24)

1. Robert, A., and R. O. Scow. 1963. Perfusion of rat adipose tissue. Am. J. Physiol 205: 405-412.
2. Scow, R. O., F. A. Stricker, T. Y. Pick, and T. R. Clary. 1965. Effect of ACTH on FFA release and diglyceride content in perfused rat adipose tissue. Ann. N. Y. Acad. Sci. 131: 288-301.
3. Chernick, S. S., R. J. Gardiner, and R. O. Scow. 1987. Restricted passage of insulin across capillary endothelium in perfused rat adipose tissue. Am. J. Physiol. 253: E475-E480.
4. Blanchette-Mackie, E. J., and R. O. Scow. 1971. Sites of lipoprotein lipase activity in adipose tissue perfused with chylomicrons. J. Cell Biol. 51: 1-25.
5. Rodbell, M., and R. O. Scow. 1965. Metabolism of chylomicrons and triglyceride emulsions by perfused rat adipose tissue. Am. J. Physiol. 208: 106-114.
6. Ho, R. J., and H. C. Meng. 1964. A technique for the cannulation and perfusion of isolated rat epididymal fat pad. J. Lipid Res. 5: 203-209.
7. Gubser, R., C. Di Francesco, and M. H. Bickel. 1986. Uptake of lipophilic model compounds into the isolated perfused rat epididymal adipose tissue. J. Pharmacol. Exp. Ther. 237: 967-971.
8. Baggen, M. G. A., R. Lammers, H. Jansen, L. Verschoor, and J. C. Birkenhäger. 1987. Effects of synacthen on lipid metabolism in the perfused epididymal fat pad of the rat. Metabolism. 36: 544-547.
9. Ho, S. J., R. J. Ho, and H. C. Meng. 1967. Comparison of heparin-released and epinephrine-sensitive lipases in rat adipose tissue. Am. J. Physiol. 212: 284-290.
10. Kuwajima, M., D. W. Foster, and J. D. McGarry. 1988. Regulation of lipoprotein lipase in different rat tissues. Metabolism. 37: 597-601.
11. Lookene, A., O. Chevreuil, P. Ostergaard, and G. Olivecrona. 1996. Interaction of lipoprotein lipase with heparin fragments and with heparan sulfate: stoichiometry, stabilization and kinetics. Biochemistry. 35: 12155-12163.
12. Crandall, D. L., G. J. Hausman, and J. G. Kral. 1997. A review of the microcirculation of adipose tissue: anatomic, metabolic, and angiogenic perspectives. Microarailation. 4: 211-232.
13. Rosell, S., and E. Belfrage. 1979. Blood circulation in adipose tissue. Proc. Rev. 59: 1078-1104.
14. Summers, L. K. M., P. Arner, V. Ilic, M. L. Clark, S. M. Humphreys, and K. N. Frayn. 1998. Adipose tissue metabolism in the postprandial period: microdialysis and arteriovenous techniques compared. Am. J. Physiol. 274: E651-E665.
15. Julve, J., M. Q. Robert, M. Llobera, and J. Peinado-Onsurbe. 1996. Hormonal regulation of lipoprotein lipase activity from 5-day-old rat hepatocytes. Mol. Cell. Endocrinol. 116: 97-104.
16. Garland, P. B., and P. J. Randle. 1962. A rapid enzymatic assay for glycerol. Nature. 196: 987-988.
17. Dauchy, R. T., D. E. Blask, and L. A. Sauer. 2000. Preparation of the inguinal fat pad for perfusion in situ in the rat: a surgical technique that preserves continuous blood flow. Contemp. Top. Lab. Anim. Sci. 39: 29-33.
18. Page, R. A., K. M. Stowell, M. J. Hardman, and K. E. Kitson. 1992. The assessment of viability in isolated rat hepatocytes. Anal. Biochem. 200: 171-175.
19. Severson, D. L., F. A. Lefebvre, and S. K. Sloan. 1980. Effect of chloroquine on rates of lipolysis in the isolated perfused rat heart and in rat epididymal fat pads. J. Mol. Cell. Cardiol. 12: 977-992.
20. Ballard, K., B. B. Fredholm, H. C. Meng, and S. Rosell. 1971. Heparin-induced release of lipase activity from perfused canine subcutaneous adipose tissue. Proc. Soc. Exp. Biol. Med. 137: 1490-1493.
21. Stewart, J. E., and M. C. Schotz. 1974. Release of lipoprotein lipase activity from isolated fat cells. J. Biol. Chem. 249: 904-907.
22. Kornhauser, D. M., and M. Vaughan. 1975. Release of lipoprotein lipase from fat cells in vitro. Biochim. Biophys. Acta. 380: 97-105.
23. Rothblat, G. H., and F. D. DeMartinis. 1977. Release of lipoprotein lipase from rat adipose tissue cells grown in culture. Biochem. Biophys. Res. Commun. 78: 45-50.
24. Sasaki, A., and I. J. Goldberg. 1992. Lipoprotein lipase release from BFC-1β adipocytes. J. Biol. Chem. 267: 15198-15204.