Primary adult human retinal pigment epithelial cell cultures on human amniotic membranes

Indian Journal of Ophthalmology

Volumn 53, Issue 2, 2005, Pages 109-113

  Singhal, Shweta ^a   Vemuganti, Geeta K ^a  

^a L V PRASAD EYE INSTITUTE   (India)

Author keywords

Adult cadaver eyeballs; Amniotic membranes; Epithelial morphology; Human retinal pigment epithelium cultures; Mechanical dissection

Indexed keywords

VISUAL PIGMENT;

AMNION; ANTERIOR EYE CHAMBER; ARTICLE; CADAVER; CELL GROWTH; CELL ISOLATION; CELL SURFACE; CELL ULTRASTRUCTURE; CHOROID CAPILLARY LAYER; COMPARATIVE STUDY; CONTROLLED STUDY; CORNEA SURGERY; CULTURE MEDIUM; ENZYME DEGRADATION; EXPLANT; EXTRACELLULAR MATRIX; EYEBANK; HUMAN; HUMAN TISSUE; IN VITRO STUDY; MONOLAYER CULTURE; PIGMENT EPITHELIUM; STUDENT T TEST; TISSUE SECTION; CELL SEPARATION; CULTURE TECHNIQUE; CYTOLOGY; DONOR; METHODOLOGY; MIDDLE AGED; PHASE CONTRAST MICROSCOPY;

AMNION; CELL CULTURE TECHNIQUES; CELL SEPARATION; HUMANS; MICROSCOPY, PHASE-CONTRAST; MIDDLE AGED; PIGMENT EPITHELIUM OF EYE; TISSUE DONORS;

EID: 23244449463     PISSN: 03014738     EISSN: 03014738     Source Type: Journal    
DOI: 10.4103/0301-4738.16174     Document Type: Article

References (23)

1. Thumann G, Hinton DR. Chapter 7-Cell biology of the retinal pigment epithelium, The Retina Vol. 1, Basic science and inherited retinal disease, Ryan SJ, Langmore SE, Hinton D, Ogden TE. London: Mosby-Year Book; 2000.
2. Young R. Pathophysiology of age related macular degeneration. Surv Ophthalmol 1987;37:291-306.
3. Sheedlo H, Li L, Turner J. Photoreceptor rescue in the dystrophic retina by transplantation of retinal pigment epithelium. Int Rev Cytol 1992;138:1-49.
4. Smith DT. The pigmented epithelium of the embryo chick's eye studied in vivo and in vitro. Bull Johns Hopkins Hosp 1920;31:239-40.
5. Mannagh J, Arya DV, Irvine AR. Tissue culture of human retinal pigment epithelium. Invest Ophthalmol 1973;12:52-3.
6. Flood MT, Gouras P, Kjeldbye H. Growth characteristics and ultrastructure of human retinal pigment epithelium in vitro. Invest Ophthalmol Vis Sci 1980;19:1309-20.
7. Mayerson PL, Hall MO, Clark V, Abrams T. An improved method for isolation and culture of rat retinal pigment epithelial cells. Invest Ophthalmol Vis Sci 1985;26:1599-609.
8. Castillo B, Little CW, Cerro CD, Cerro MD. An improved method of isolating fetal human retinal pigment epithelium. Curr Eye Res 1995;14:677-83.
9. Von Rekum HA, Okano T, Kim SW, Bernstein PS. Maintenance of retinoid metabolism in human retinal pigment epithelial cell culture. Exp Eye Res 1999;69:97-107.
10. Lu L, Kam LC, Hasenbein M, Bizios R, Mikos AG. Retinal pigment epithelial cell function on substrates with chemically micropatterned surfaces. Biomaterials 1999;20:2351-61.
11. Farrokh-Siar, Rezai KA, Patel SC, Earnest JT. Cryoprecipitate: An autologous substrate for human fetal retinal pigment epithelium. Curr Eye Res 1999;19:89-94.
12. Dutt K, Scott MM, Del Monte M, Brenman M, Hanis-Hookns, Kaplan HJ, et al. Extracellular matrix mediated growth and differentiation in human retinal pigment epithelial cell line 0041. Curr Eye Res 1991;10:1089-100.
13. Capeans C, Pineiro A, Pardo M, et al. Amniotic membrane as support for human retinal pigment epithelium (RPE) cell growth. Acta Ophthalmol Scand 2003;81:271-7.
14. Meller D, Tseng SCG. In vitro conjunctival epithelial differentiation on preserved human amniotic membrane. Invest Ophthalmol Vis Sci 1998;39:S428-9.
15. Cho B, Djalilian AR, Obstrisch WF, Matteson DM, Chan CC, Holland EJI. Conjunctival epithelial cells cultured on human amniotic membrane do not transdifferentiate into corneal epithelial type cells. Invest Ophthalmol Vis Sci 1998;39:S428-9.
16. Nakumura T, Endo KI, Cooper LJ, Fullwood NJ, Tanifuji N, Tsuzuki M, et al. The successful culture and autologous transplantation of rabbit oral mucosal epithelial cells on amniotic membrane. Invest Ophthalmol Vis Sci 2003;44:106-16.
17. Meller D, Pires RTF, Tseng SCG. Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures. Br J Ophthalmol 2002;86:963-71.
18. Sangwan VS, Vemuganti GK, Iftekhar G, Bansal AK, Rao GN. Use of autologous cultured limbal and conjunctival epithelium in a patient with severe bilateral ocular surface disease induced by acid injury. Cornea 2003;22:478-81.
19. Vemuganti GK, Kashyap S, Sangwan VS, Singh S. Ex-vivo potential of cadaveric and fresh limbal tissues to regenerate cultured epithelium. Indian J Ophthalmol 2004;52:113-20.
20. Sangwan VS, Vemuganti GK, Singh S, Balasubramanian D. Successful reconstruction of damaged ocular outer surface in humans using limbal and conjunctival stem cell culture methods. Biosci Rep 2003;23:169-74.
21. Grisanti S, Guidry C. Transdifferentiation of retinal pigment epithelial cells from epithelial to mesenchymal phenotype. Invest Ophthalmol Vis Sci 1995;36:391-405.
22. Ishida M, Lui GM, Yamani A, Sugina IK, Zarbin MA. Culture of human retinal pigment epithelial cells from peripheral scleral flap biopsies. Curr Eye Res 1998;17:392-402.
23. Nguyen MT, Arnheiter H. Signaling and transcriptional regulation in early mammalian eye development: A link between FGF and MITF. Development 2000;127:3581-91.