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note
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After many hours of UV excitation, we detect some changes in the emission spectrum due to photobleaching. We were careful to change samples before any degradation occurs.
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30
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23144453439
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note
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The purity of the sample was checked by thin layer chromatography (TLC). In addition, we performed an experiment by titrating the 2G2-m-Per with 2G2-m-OH, comparing absorption and emission spectra as a function of added 2G2-m-OH. We conclude that the spectroscopy experiments are more sensitive than TLC and can detect 1-2% impurity. A concentration of 1% 2G2-m-OH yields at least 99% of the integrated fluorescence of the band peaked at 435 nm seen in Figure 3.
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0345687198
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