LightTyper assay with locked-nucleic-acid-modified oligomers for genotyping of the toll-like receptor 4 polymorphisms A896G and C1196T

Clinical Chemistry

Volumn 51, Issue 8, 2005, Pages 1523-1525

  Grannemann, Silke ^a   Landt, Olfert ^b   Breuer, Sebastian ^c   Blömeke, Brunhilde ^c  

^a UNIVERSITY HOSPITAL RWTH AACHEN   (Germany)

^b TIB MOLBIOL GmbH   (Germany)

^c UNIVERSITY OF TRIER   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

LOCKED NUCLEIC ACID MODIFIED OLIGOMER; OLIGOMER; TOLL LIKE RECEPTOR 4; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; GENETIC POLYMORPHISM; GENETIC VARIABILITY; GENOTYPE; HUMAN; HUMAN CELL; MAJOR CLINICAL STUDY; NUCLEIC ACID ANALYSIS; PROTEIN DETERMINATION; PROTEIN DOMAIN; SIGNAL DETECTION;

BASE SEQUENCE; FLUORESCENT DYES; GENOTYPE; HUMANS; MEMBRANE GLYCOPROTEINS; MUTATION; OLIGONUCLEOTIDES, ANTISENSE; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; RECEPTORS, CELL SURFACE; TOLL-LIKE RECEPTOR 4; TOLL-LIKE RECEPTORS;

EID: 23044510079     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2005.049197     Document Type: Article

References (12)

1. Muzio M, Bosisio D, Polentarutti N, D'Amico G, Stoppacciaro A, Mancinelli R, et al. Differential expression and regulation of toll-like receptors (TLR) in human leukocytes: selective expression of TLR3 in dendritic cells. J Immunol 2000;164:5998-6004.
2. Franchimont D, Vermeire S, El Housni H, Pierik M, Van Steen K, Gustot T, et al. Deficient host-bacteria interactions in inflammatory bowel disease? The toll-like receptor (TLR)-4 Asp299Gly polymorphism is associated with Crohn's disease and uloerative colitis. Gut 2004;53:987-92.
3. Palsson-McDermott EM O'Neill LA. Signal transduction by the lipopolysaccharide receptor, Toll-like receptor-4. Immunology 2004;113:153-62.
4. Arbour NC, Lorenz E, Schutte BC, Zabner J, Kline JN, Jones M, et al. TLR4 mutations are associated with endotoxin hyporesponsiveness in humans. Nat Genet 2000;25:187-91.
5. Lorenz E, Frees KL, Schwartz DA. Determination of the TLR4 genotype using allele-specific PCR. Biotechniques 2001;31:22-4.
6. Schmitt C, Humeny A, Becker CM, Brune K, Pahl A. Polymorphisms of TLR4: rapid genotyping and reduced response to lipopolysaccharide of TLR4 mutant alleles. Clin Chem 2002;48:1661-7.
7. Heesen M, Wessiepe M, Kunz D, Vasickova K, Blomeke B. Rapid and reliable genotyping for the Toll-like receptor 4 A896G polymorphism using fluorescence-labeled hybridization probes in a real-time polymerase chain reaction assay. Clin Chim Acta 2003;333:47-9.
8. Arjomand-Nahad F, Diefenbach K, Landt O, Gaikovitch E, Roots I. Genotyping of the triallelic variant G2677T/A in MDR1 using LightCycler with locked-nucleic-acid-modified hybridization probes. Anal Biochem 2004;334:201-3.
9. Mouritzen P, Nielsen AT, Pfundheller HM, Choleva Y, Kongsbak L, Moller S. Single nucleotide polymorphism genotyping using locked nucleic acid (LNA). Expert Rev Mol Diagn 2003;3:27-38.
10. Hamann L, Hamprecht A, Gomma A, Schumann RR. Rapid and inexpensive real-time PCR for genotyping functional polymorphisms within the Toll-like receptor-2, -4, and -9 genes. J Immunol Methods 2004;285:281-91.
11. Okayama N, Fujimura K, Suehiro Y, Hamanaka Y, Fujiwara M, Matsubara T, et al. Simple genotype analysis of the Asp299Gly polymorphism of the Toll-like receptor-4 gene that is associated with lipopolysaccharide hyporesponsiveness. J Clin Lab Anal 2002;16:56-8.
12. Michel O, LeVan TD, Stern D, Dentener M, Thorn J, Gnat D, et al. Systemic responsiveness to lipopolysaccharide and polymorphisms in the toll-like receptor 4 gene in human beings. J Allergy Clin Immunol 2003;112:923-9.