Use of low-melting-point primers for bisulfite genomic sequencing: Analysis of the H37 lung cancer tumor suppressor gene promoter

Analytical Biochemistry

Volumn 343, Issue 2, 2005, Pages 347-349

  Oh, Juliana J ^a   Boctor, Baher ^a   Jimenez, Cynthia A ^a   Lopez, Roberto C ^a   Razfar, Ali ^a   Slamon, Dennis J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

GENOMIC SEQUENCING; LOW MELTING POINT; LUNG CANCER;

GENES;

GENOMIC DNA;

AMPLICON; ARTICLE; DNA METHYLATION; DNA TEMPLATE; GENE AMPLIFICATION; GENE SEQUENCE; GENOME ANALYSIS; LUNG CANCER; MELTING POINT; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; TUMOR SUPPRESSOR GENE;

EID: 23044507020     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.05.008     Document Type: Article

References (9)

1. S.E. Jacobsen, H. Sakai, E.J. Finnegan, X. Cao, and E.M. Meyerowitz Ectopic hypermethylation of flower-specific genes in Arabidopsis Curr. Biol. 10 2000 179 186
2. A.M. Raizis, F. Schmitt, and J.P. Jost A bisulfite method of 5-methylcytosine mapping that minimizes template degradation Anal. Biochem. 226 1995 161 166
3. S.J. Clark, J. Harrison, C.L. Paul, and M. Frommer High sensitivity mapping of methylated cytosines Nucleic Acids Res. 22 1994 2990 2997
4. G.E. Tusnady, I. Simon, A. Varadi, and T. Aranyi BiSearch: primer-design and search tool for PCR on bisulfite-treated genomes Nucleic Acids Res. 33 2005 e9
5. J.J. Oh, A.R. West, M.C. Fishbein, and D.J. Slamon A candidate tumor suppressor gene, H37, from the human lung cancer tumor suppressor locus 3p21.3 Cancer Res. 62 2002 3207 3213
6. R. Paulin, G.W. Grigg, M.W. Davey, and A.A. Piper Urea improves efficiency of bisulphite-mediated sequencing of 5′-methylcytosine in genomic DNA Nucleic Acids Res. 26 1998 5009 5010
7. A. Olek, J. Oswald, and J. Walter A modified and improved method for bisulphite based cytosine methylation analysis Nucleic Acids Res. 24 1996 5064 5066
8. R.H. Don, P.T. Cox, B.J. Wainwright, K. Baker, and J.S. Mattick 'Touchdown' PCR to circumvent spurious priming during gene amplification Nucleic Acids Res. 19 1991 4008
9. Y. Nagane, K. Utsugisawa, and H. Tohgi PCR amplification in bisulfite methylcytosine mapping in the GC-rich promoter region of amyloid precursor protein gene in autopsy human brain Brain Res. Protoc. 5 2000 167 171