Flow cytometry based techniques to study testicular acidophilic granulocytes from the protandrous fish gilthead seabream (Sparus aurata L.)

Biological Procedures Online

Volumn 6, Issue 1, 2004, Pages 129-136

  Chaves Pozo, Elena ^a   Mulero, Victoriano ^a   Meseguer, José ^a   García Ayala, Alfonsa ^a  

^a UNIVERSITY OF MURCIA   (Spain)

Author keywords

Flow cytometry; Phagocytosis; Testis

Indexed keywords

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL FUNCTION; CELL ISOLATION; CELL TYPE; CONTROLLED STUDY; FLOW CYTOMETRY; GRANULOCYTE; INVOLUTION; NONHUMAN; SEA BREAM; TELEOST; TESTIS; TESTIS CELL; TESTIS DEVELOPMENT;

SPARUS AURATA; TELEOSTEI;

EID: 23044500140     PISSN: 14809222     EISSN: None     Source Type: Journal    
DOI: 10.1251/bpo81     Document Type: Article

References (21)

1. Hess RA. Spermatogenesis, overview. In: Knobil E, Neil JD, editors. Encyclopedia of Reproduction volumen 4. New York: Academic Press; 1999. p.539-545.
2. Miura T. Spermatogenetic cycle in fish. In: Knobil E, Neil JD, editors. Encyclopedia of Reproduction volumen 4. New York: Academic Press; 1999. p. 571-578.
3. Hales DB. Testicular macrophage modulation of Leydig cell steroidogenesis. J Reprod Immunol 2002; 57:3-18.
4. Loir M. In vitro approach to the control of spermatogonia proliferation in the trout. Mol Cell Endocrinol 1994; 102:141-150.
5. Miura T, Yamauchi K, Takahashi H, Nagahama Y. Hormonal induction of all stages of spermatogenesis in vitro in the male Japanese eel (Anguilla japonica). Proc Natl Acad Sci USA 1991; 88:5774-5778.
6. Loir M, Sourdaine P. Testes cells: isolation and culture. In: Hochachka, Mommsen editors. Biochemistry and Molecular Biology of Fishes volumen 3. Elsevier Science Press; 1994. p. 249-272.
7. Sepulcre MP, Pelegrín P, Mulero V, Meseguer J. Characterisation of gilthead seabream acidophilic granulocytes by a monoclonal antibody unequivocally points to their involvement in fish phagocytic response. Cell Tissue Res 2002; 308:97-102.
8. Esteban MA, Mulero V, Muñoz J, Meseguer J. Methodological aspects of assesing phagocytosis of Vibrio anguillarum by leucocytes of gilthead seabream (Sparus aurata L.) by flow cytometry and electron microscopy. Cell Tissue Res 1998; 293:133-141.
9. Stanley H, Chieffi G, Botte V. Histological and histochemical observations on the testis of Gobius paganellus. Z Zellforsch 1965; 65:350-362.
10. Shrestha TK, Khanna SS. Histology and seasonal changes in the testes of a hill-stream fish Schizothorax plagiostomus. Z Mikrosk Anat Forsch 1976; 90:749-761.
11. Shrestha TK, Khanna SS. Seasonal changes in the testes of a hill stream teleost, Garra gotyla (Gray). Acta Anat 1978; 100:210-220.
12. Micale V, Perdichizzi F, Santangel G. The gonadal cycle of captive white bream, Diplodus sargus (L.). J Fish Biol 1986; 31:435-440.
13. Besseau L, Faliex E. Resorption of unemitted gametes in Lithognathus mormyrus (Sparidae, Teleostei): a possible synergic action of somatic and immune cells. Cell Tissue Res 1994; 276:123-132
14. Bruslé-Sicard S, Fourcault B. Recognition of sex-inverting protandric Sparus aurata: ultrastructural aspect. J Fish Biol 1997; 50:1094-1103.
15. Chaves-Pozo E, Pelegrén P, Mulero V, Meseguer J, García Ayala A. A role for acidophilic granulocytes in the testis of the gilthead seabream (Sparus aurata L., Teleostei). J Endocrinol 2003; 179:165-174.
16. Van den Hurk R, Peute J, Vermeij JA. Morphological and enzyme cytochemical aspects of the testis and vas deferens of the rainbow trout, Salmo gairdneri. Cell Tissue Res 1978; 186:309-325.
17. Billard R, Takashima F. Resorption of spermatozoa in the sperm duct of rainbow trout during the post-spawning period. Bull Japan Soc Sci Fish 1983; 49:387-392.
18. Scott AP, Sumpter JP. Seasonal variations in testicular germ cell stages and in plasma concentrations of sex steroids in male rainbow trout (Salmo gairdneri) maturing at 2 years old. Gen Comp Endocrinol 1989; 73:46-58.
19. Meseguer J, Esteban MA, Muñoz J, López-Ruiz A. Ultrastructure of the peritoneal exudate cells of seawater teleosts, seabream (Sparus aurata) and sea bass (Dicentrarchus labrax). Cell Tissue Res 1993; 273:301-307.
20. López-Ruiz A, Esteban MA, Meseguer J. Blood cells of the gilthead seabream (Sparus aurata L.). Light and electron microscopic studies. Anat Rec 1992; 234:161-171.
21. Miura T, Ando N, Miura C, Yamauchi K. Comparative studies between in vivo and in vitro spermatogenesis of Japanese eel (Anguilla japonica). Zoolog Sci 2002; 19:321-329.