Miniemulsion droplets as single molecule nanoreactors for polymerase chain reaction

Biomacromolecules

Volumn 6, Issue 4, 2005, Pages 1824-1828

  Musyanovych, Anna ^a   Mailänder, Volker ^b   Landfester, Katharina ^a  

^a UNIVERSITY OF ULM   (Germany)

^b German Red Cross Blood Transfusion Service Baden Württemberg Hessen and University Hospital Ulm   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOREACTORS; CONCENTRATION (PROCESS); DROP FORMATION; MICROEMULSIONS; NANOSTRUCTURED MATERIALS; PARTICLE SIZE ANALYSIS;

MINIEMULSION DROPLETS; NANOREACTORS; POLYMERASE CHAIN REACTION (PCR); SONICATION PARAMETERS;

ENZYMES;

DNA; TAQ POLYMERASE; WATER OIL CREAM;

ARTICLE; BIOCHEMISTRY; CHEMICAL REACTION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA DETERMINATION; EMULSION; PARTICLE SIZE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REACTOR DESIGN; TEMPERATURE; TIME; ULTRASOUND;

BIOREACTORS; EMULSIONS; MICROSCOPY, ATOMIC FORCE; NANOTECHNOLOGY; POLYMERASE CHAIN REACTION; SURFACE-ACTIVE AGENTS;

EID: 22944461454     PISSN: 15257797     EISSN: None     Source Type: Journal    
DOI: 10.1021/bm050084+     Document Type: Article

References (12)

1. Müller, H.-J. In PCR-Polymerase-Kettenreaktion; Müller, H.-J., Ed.; Spektrum Akademischer Verlag GmbH: Berlin, 2001.
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6. Tawfik, D. S.; Griffiths, A. D. Nat. Biotechnol. 1998, 16, 652-656.
7. Landfester, K Miniemulsions for nanoparticle synthesis. In Colloid Chemistry; Antonietti, M., Ed.; Spinger: Heidelberg, Germany, 2003; pp 75-124.
8. Schork, F. J.; Poehlein, G. W.; Wang, S.; Reimers, J.; Rodrigues, J.; Samer, C. Colloids Surf. 1999, A 153, 39-45.
9. Landfester, K. Macromol. Symp. 2000, 150, 171-178.
10. Landfester, K. Adv. Mater. 2001, 13, 765-768.
11. Oligonucleotide primer pairs were designed using on line available resources (http://bioweb.pasteur.fr/seqanal/interfaces/eprimer3.html) to minimize the possibility of self-dimerisation that could lead to nonspecific PCR amplicons. Primers were purchased in a lyophilized form from Thermo Electron GmbH Germany, then diluted to a final concentration of 100 μM with sterile distilled water and stored at -20 °C until use.
12. The dsDNA template (286bp) for miniemulsion-PCR is a product of a classical PCR reaction in solution and was synthesized from genomic human dsDNA (kindly provided by Dr. Kaimo Hirv, Institute of Transfusion Medicine and Immunogenetics, Ulm) using 5′-CGGCAGCAACAGCAGGT-3 as forward and 5′-CCACGC-CAATCACTCTCATCT-3 as reversible primers. These primers amplify a short sequence of a housekeeping gene (PGBD.; GeneBank AccessionNo. M95623; Primers were designed as described in ref 11).