Prion propagation in cell culture

Methods in Molecular Biology

Volumn 299, Issue , 2005, Pages 227-234

  Lehmann, Sylvain ^a,b  

^a CNRS   (France)

^b HÔPITAL SAINT ELOI   (France)

Author keywords

Cell culture; Creutzfeldt Jakob disease; Murine neuroblastoma cell line N2a; Prion; Protease resistance; PrP; Scrapie; Transmissible spongiform encephalopathies

Indexed keywords

BIOCHEMICAL MARKER; PROTEINASE K;

BRAIN HOMOGENATE; BRAIN INFECTION; CELL CULTURE; DISEASE TRANSMISSION; NEURO-2A CELL LINE; NONHUMAN; PRION; PRION DISEASE;

EID: 22844447952     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1385/1-59259-874-9:227     Document Type: Chapter

References (16)

1. Béranger, F., Mangé, A., Solassol, J., and Lehmann, S. (2001) Cell culture models of Prion Diseases. Biochem. Biophys. Res. Communications 289, 311-316.
2. Somerville, R. A., Chong, A., Mulqueen, O. U., Birkett, C. R., Wood, S. C., and Hope, J. (1997) Biochemical typing of scrapie strains. Nature. 386, 564.
3. Lehmann, S., Laude, H., Harris, D. A., et al. (2001) Ex vivo transmission of mouse adaptedprion strains to N2a and GT1-7 cell lines, in Alzheimer's Disease: Advances in Etiology, Pathogenesis and Therapeutics (K. Iqbal, S. S. Sisodia, and B. Winblad, eds.), John Wiley & Sons, Ltd., UK.
4. Nishida, N., Harris, D. A., Vilette, D., et al. (2000) Successful transmission of three mouse-adapted scrapie strains to murine neuroblastoma cell lines overexpressing wild-type mouse prion protein. J. Virol. 74, 320-325.
5. Bosque, P. J. and Prusiner, S. B. (2000) Cultured cell sublines highly susceptible to prion infection. J. Virol. 74, 4377-4386.
6. Taraboulos, A., Serban, D., and Prusiner, S. B. (1990) Scrapie prion proteins accumulate in the cytoplasm of persistently infected cultured cells. J. Cell Biol. 110, 2117-2132.
7. Winklhofer, K. F., Hartl, F. U., and Tatzelt, J. (2001) A sensitive filter retention assay for the detection of PrP(Sc) and the screening of anti-prion compounds. FEBSLett. 503,41-45.
8. Klohn, P. C., Stoltze, L., Flechsig, E., Enari, M., and Weissmann, C. (2003) A quantitative, highly sensitive cell-based infectivity assay for mouse scrapie prions. Proc. Natl. Acad. Sci. USA 100, 11666-11671.
9. Flechsig, E., Hegyi, I., Enari, M., Schwarz, P., Collinge, J., and Weissmann, C. (2001) Transmission of scrapie by steel-surface-bound prions. Mol. Med. 7, 679-684.
10. Caughey, B. and Raymond, G. J. (1993) Sulfated polyanion inhibition of scrapieassociated PrP accumulation in cultured cells. J. Virol. 67, 643-650.
11. Mange, A., Nishida, N., Milhavet, O., McMahon, H. E. M., Casanova, D., and Lehmann, S. (2000) Amphotericin B inhibits the generation of the scrapie isoform of the prion protein in infected cultures. J. Virol. 74, 3135-3140.
12. Kocisko, D. A., Baron, G. S., Rubenstein, R., Chen, J., Kuizon, S., and Caughey, B. (2003). New inhibitors of scrapie-associated prion protein formation in a library of 2000 drugs and natural products. J. Virol. 77, 10288-10294.
13. Korth, C., May, B. C., Cohen, F. E., and Prusiner, S. B. (2001). Acridine and phenothiazine derivatives as pharmacotherapeutics for prion disease. Proc. Natl. Acad. Sci. USA 98, 9836-9841.
14. Rudyk, H., Vasiljevic, S., Hennion, R. M., Birkett, C. R., Hope, J., and Gilbert, I. H. (2000). Screening Congo Red and its analogues for their ability to prevent the formation of PrP-res in scrapie-infected cells. J. Gen. Virol. 81, 1155-1164.
15. Supattapone, S., Nguyen, H. O., Cohen, F. E., Prusiner, S. B., and Scott, M. R. (1999). Elimination of prions by branched polyamines and implications for therapeutics. Proc. Natl. Acad. Sci. USA 96, 14529-14534.
16. Perrier, V., Wallace, A. C., Kaneko, K., Safar, J., Prusiner, S. B., and Cohen, F. E. (2000). Mimicking dominant negative inhibition of prion replication through structure-based drug design. Proc. Natl. Acad. Sci. USA 97, 6073-6078.