Active site of arginine kinase from sea cucumber Stichopus japonicus by site mutagenesis

Qinghua Daxue Xuebao/Journal of Tsinghua University

Volumn 45, Issue 6, 2005, Pages 858-861

  Wang, Zheng ^a   Guo, Shuyuan ^a   Zhang, Jianwei ^a   Wang, Xicheng ^a  

^a TSINGHUA UNIVERSITY   (China)

Author keywords

Active site; Arginine kinase; Site mutagenesis

Indexed keywords

ACTIVATION ANALYSIS; CATALYST ACTIVITY; FUNCTIONS; INCLUSIONS; METABOLISM; MUTAGENESIS; ORGANIC COMPOUNDS; PROTEINS; STRUCTURES (BUILT OBJECTS);

ACTIVE SITE; ARGININE KINASE; SITE MUTAGENESIS; STICHOPUS JAPONICUS;

BIOLOGICAL MATERIALS;

EID: 22844443397     PISSN: 10000054     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (10)

1. Ellington W R, Bush J. Cloing and expression of a lombricine kinase from an echiuroid worm: Insights into structural correlates of substrate specificity[J]. Biochem Biophys Res Commun, 2002, 291(4): 939-944.
2. Suzuki T, Kamidochi M, Inoue N, et al. Arginine kinase evolved twice: evidence that echinoderm arginine kinase originated from creatine kinase[J]. Biochem J, 1999, 340(Pt3): 671-675.
3. Chen L H, Borders C L, Vasquez J R, et al. Rabbit muscle creatine kinase: consequences of the mutagenesis of conserved histidine residues[J]. Biochemistry, 1996, 35(24): 7895-7902.
4. Wang P F, McLeish M J, Kneen M M, et al. An unusually low pK(a) for Cys282 in the active site of human muscle creatine kinase[J]. Biochemistry, 2001, 40(39): 11698-11705.
5. Buechter D D, Medzihradszky K F, Burlingame A L, et al. The active site of creatine kinase. Affinity labeling of cysteine 282 with N-(2,3-epoxypropyl)-N-amidinoglycine[J]. J Biol Chem, 1992, 267(4): 2173-2178.
6. Lin L, Ferryman M B, Friedman D, et al. Determination of the catalytic site of creatine kinase by site-directed mutagenesis[J]. Biochim Biophys Acta, 1994, 1206(1): 97-104.
7. Zhou G, Somasundaram T, Blanc E, et al. Transition state of arginine kinase: Implication for catalysis of bimolecular reactions[J]. Proc Natl Acad Sci U.S.A, 1998, 95(15): 8449-8454.
8. Guo Q, Chen B Y, Wang X C. Evidence for the proximal cysteine and lysine residue present at or near the active site of arginine kinase in Stichopus japonicus[J]. Biochemistry (Moscow), Accepted.
9. Guo S Y, Guo Z, Guo Q, et al. Expression, purification and characterization of arginine kinase from the sea cucumber Stichopus japonicus[J]. Protein Express Purif, 2003, 29(2): 230-234.
10. Chen B Y, Guo Q, Guo Z, et al. Improved activity assay method for arginine kinase based on a ternary heteropolyacid system[J]. Tsinghua Science and Technology, 2003, 8(4): 422-427.