Cloning, physical mapping and expression analysis of a wheat Mlo-like gene

Journal of Integrative Plant Biology

Volumn 47, Issue 2, 2005, Pages 214-222

  Yu, Ling ^a,b   Niu, Ji Shan ^a,c   Chen, Pei Du ^a   Ma, Zheng Qiang ^a   Liu, Da Jun ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

^b NORTHWEST NORMAL UNIVERSITY   (China)

^c HENAN AGRICULTURAL UNIVERSITY   (China)

Author keywords

Cloning; Expression; Mlo like gene; RT PCR; Triticum aestivum Haynaldia villosa 6VS 6AL translocation line

Indexed keywords

GENE EXPRESSION;

BLUMERIA GRAMINIS; HAYNALDIA VILLOSA; HORDEUM VULGARE; HORDEUM VULGARE SUBSP. VULGARE; TRITICUM AESTIVUM;

EID: 22744442407     PISSN: 16729072     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1744-7909.2005.00030.x     Document Type: Article

References (20)

1. Ausubel FM, Brent R, Kingston RE et al. (1995). Short Protocols in Molecular Biology, 3rd edn. John Wiley & Sons, Media, PA.
2. Jarosch B, Kogel K-H, Schaffrath U (1999). The ambivalence of the barley mlo locus: Mutations conferring resistance against powdery mildew (Blumeria graminis f. sp. hrodei) enhance susceptibility to the rice blast fungus Magnaporthe grisea. Mol Plant Microbe In 12, 508-514.
3. Büschges R, Hollricher K, Panstruga R et al. (1997). The barley Mlo gene: A novel control element of plant pathogen resistance. Cell 88, 695-705.
4. Chen PD, Qi LL, Zhou B, Zhang SZ, Liu DJ (1995). Development and molecular cytogenetic analysis of wheat: Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew. Theor Appl Genet 91, 1125-1128.
5. Devoto A, Piffanelli P, Nillsson I et al. (1999). Topology, subcellular localization, and sequence diversity of the Mlo family in plants. J Biol Chem 274, 34993-35004.
6. Devoto A, Hartmann HA, Piffanelli P et al. (2003). Molecular phylogeny and evolution of the plant-specific seven-transmembrane MLO family. J Mol Evol 56, 77-88.
7. Elliott C, Zhou F, Spielmeyer W, Panstruga R, Schulze-Lefert P (2002). Functional conservation of wheat and rice Mlo orthologs in defense modulation to the powdery mildew fungus. Mol Plant Microbe In 15, 1069-1077.
8. Hammond-Kosack KE, Jones JDG (1996). Resistance gene dependent plant defense response. Plant Cell 8, 1773-1791.
9. Hinze K, Thompson RD, Ritter E, Salamini F, Schulze-Lefert P (1991). RFLP-mediated targeting of the mlo resistance locus in barley (Hordeum vulgare). Proc Natl Acad Sci USA 88, 3691-3695.
10. Li WL, Faris JD, Chittor JM et al. (1999). Genomic mapping of defense response gene in wheat. Theor Appl Genet 98, 226-233.
11. Ma ZQ, Sorrells ME (1995). Genetic analysis of fertility restoration in wheat using restriction fragment length polymorphism. Crop Sci 35, 1137-1143.
12. McIntosh RA, Devos KM, Dubcovsky J, Morris CF, Rogers WJ (2003). Catalogue of gene symbols for wheat: 2003 Supplement. Available on-line: http://wheat.pw.usda.gov/ggpages/wgc.
13. Niu JS, Yu L, Ma ZQ, Chen PD, Liu DJ (2002). Molecular cloning, characterization and mapping of a rhodancse-like gene in wheat. Acta Genet Sin 29, 266-272.
14. Piffanelli P, Zhou F, Casais C et al. (2002). The barley MLO modulator of defense and cell death is responsive to biotic and abiotic stress stimuli. Plant Physiol 129, 1076-1085.
15. Sambrook L, Fritsch EF, Manniatis T (1989). Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York.
16. Shirasu K, Nielsen K, Piffanelli P, Oliver R, Schulze-Lefert P (1999). Cell-autonomous complementation of mlo resistance using a biolistic transient expression system. Plant J 17, 293-299.
17. Simons G, van Der Lee T, Diergaarde P et al. (1997). AFLP-based fine mapping of the Mlo gene to a 30 kb DNA segment of the barley genome. Genomics 44, 61-70.
18. Staskawicz BJ, Ausubel FM, Baker BJ, Ellis JG, Jones JDG (1995). Molecular genetics of plant disease resistance. Science 268, 661-667.
19. Wolter M, Hollricher K, Salamini F, Schulze-Lefert P (1993). The mlo resistance alleles to powdery mildew infection in barley trigger a developmentally controlled defense mimic phenotype. Mol Gen Genet 239, 122-128.
20. Yoshida S, Uemura M, Niki T, Sakai A, Gusta LV (1983). Partition of membrane particles in aqueous two-polymer phase system and its partial use for purification of plasma membranes from plants. Plant Physiol 72, 105-114.