Solution structure of the fifth and sixth transmembrane segments of the mitochondrial oxoglutarate carrier

Molecular Membrane Biology

Volumn 22, Issue 3, 2005, Pages 191-201

  Castiglione Morelli, Maria A ^a   Ostuni, Angela ^a   Croce, Franca ^a   Palmieri, Ferdinando ^b,c   Bisaccia, Faustino ^a  

^a UNIVERSITY OF BASILICATA   (Italy)

^b UNIVERSITY OF BARI   (Italy)

^c CNR   (Italy)

Author keywords

Mitochondria; NMR; Oxoglutarate carrier; Structure

Indexed keywords

MEMBRANE PROTEIN; OXOGLUTARATE CARRIER; SYNTHETIC PEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; CIRCULAR DICHROISM; FLUORESCENCE SPECTROSCOPY; GEOMETRY; HYDROPHILICITY; MITOCHONDRIAL MEMBRANE; MITOCHONDRION; PEPTIDE ANALYSIS; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; ANIMALS; CATTLE; CIRCULAR DICHROISM; HYDROGEN BONDING; MEMBRANE TRANSPORT PROTEINS; MODELS, MOLECULAR; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN STRUCTURE, SECONDARY;

BOVINAE;

EID: 22544442794     PISSN: 09687688     EISSN: None     Source Type: Journal    
DOI: 10.1080/09687860500063522     Document Type: Article

References (37)

1. Bax A, Davis DG. 1985. MLEV-17-based two-dimensional homonuclear magnetization transfer spectroscopy. J Magn Reson 65:355-366.
2. Bhattacharyya R, Chakrabarti P. 2003. Stereospecific interactions of proline residues in protein structures and complexes. J Mol Biol 331:925-940.
3. Bisaccia F, Capobianco L, Brandolin G, Palmieri F. 1994. Transmembrane topography of the mitochondrial oxoglutarate carrier assessed by peptide-specific antibodies and enzymatic cleavage. Biochemistry 33:3705-3713.
4. Bisaccia F, Zara V, Capobianco L, lacobazzi V, Mazzeo M, Palmieri F. 1996. The formation of a disulfide cross-link between the two subunits demonstrates the dimeric structure of the mitochondrial oxoglutarate carrier. Biochim Biophys Acta 1292:281-288.
5. Bywater RP, Thomas D, Vriend G. 2001. A sequence and structural study of transmembrane helices. J Comput Aided Mol Des 15:533-552.
6. Buck M. 1998. Trifluoroethanol and colleagues: Cosolvents come of age. Recent studies with peptides and proteins. Q Rev Biophys 31:297-355.
7. Castiglione Morelli MA, Ostuni A, Pepe A, Lauria G, Palmieri F, Bisaccia F. 2004. Solution structure of the first and second transmembrane segments of the mitochondrial oxoglutarate carrier. Mol Membr Biol 21:297-305.
8. Chopra A, Yeagle PL, Alderfer JL, Albert AD. 2000. Solution structure of the sixth transmembrane helix of the G-protein-coupled receptor, rhodopsin. Biochim Biophys Acta 1463:1-5.
9. Chou PY, Fasman GD. 1974. Conformational parameters for amino acids in helical, beta-sheet, and random coil regions calculated from proteins. Biochemistry 13:211-222.
10. Deléage G, Clerc FF, Roux B, Gautheron DC. 1988. ANTHE-PROT: A package for protein sequence analysis using a microcomputer. Comput Appl Biosci 4:351-356.
11. Guntert P, Mumenthaler M, Wüthrich K. 1997. Torsion angle dynamics for NMR structure calculation with the new program DYANA. J Mol Biol 273:283-298.
12. Jeener J, Meier BH, Bachmann P, Ernst RR. 1979. Investigation of exchange processes by two-dimensional NMR spectroscopy. J Chem Phys 71:4546-4553.
13. Katragadda M, Alderfer JL, Yeagle PL. 2000. Solution structure of the loops of bacteriorhodopsin closely resembles the crystal structure. Biochim Biophys Acta 1466:1-6.
14. Katragadda M, Alderfer JL, Yeagle PL. 2001. Assembly of a polytopic membrane protein structure from the solution structures of overlapping peptide fragments of bacteriorhodopsin. Biophys J 81:1029-1036.
15. King DS, Fields CG, Fields GB. 1990. A cleavage method which minimizes side reactions following Fmoc solid phase peptide synthesis. Int J Peptide Protein Res 36:255-266.
16. Koradi R, Billeter M, Wüthrich K. 1996. MOLMOL: A program for display and analysis of macromolecular structures. J Mol Graphics 14:51-55.
17. Krämer R, Palmieri F. 1992. Metabolite carriers in mitochondria. In: Ernster L, editor. Molecular mechanisms in bioenergetics. Amsterdam: Elsevier Science BV. p 359-384.
18. 1H chemical shifts obtained as a function of temperature and trifluoroethanol concentration for the peptide series GGXGG. J Biomol NMR 5:14-24.
19. Morozzo della Rocca B, Lauria G, Venerini F, Palmieri L, Polizio F, Capobianco L, Stipani V, Pedersen J, Cappello AR, Desideri A, Palmieri F. 2003. The mitochondrial oxoglutarate carrier: Structural and dynamic properties of transmembrane segment IV studied by site-directed spin labelling. Biochemistry 42:5493-5499.
20. Palmieri F. 1994. Mitochondrial carrier proteins. FEBS Lett 346:48-54.
21. Palmieri F, Bisaccia F, Capobianco L, Dolce V, Fiermonte G, lacobazzi V, Indiveri C, Palmieri L. 1996. Mitochondrial metabolite transporters. Biochim Biophys Acta 1275:127-132.
22. Palmieri F. 2004. The ABC of solute carriers. Pflugers Arch Europ J Physiol 447:689-709.
23. Pearce SF, Hawrot E. 1990. Intrinsic fluorescence of binding-site fragments of the nicotinic acetylcholine receptor: Perturbations produced upon binding α-bungrotoxin. Biochemistry 29:10649-10659.
24. Pebay-Peyroula CE, Dahou-Gonzales C, Kahn R, Trezeguet V, Lauquin GJM, Brandolin G. 2003. Structure of mitochondrial ADP/ATP carrier in complex with carboxyatractyloside. Nature 426:39-43.
25. Piantini U, Sorensen OW, Ernst RR. 1982. Multiple quantum filters for elucidating NMR coupling networks. J Am Chem 169:949-961.
26. Piotto M, Saudek V, Sklenar V. 1992. Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions. J Biomol NMR 2:661-665.
27. Raussens V, Slupsky CM, Ryan RO, Sykes BD. 2002. NMR structure and dynamics of a receptor-active apolipoprotein E peptide. J Biol Chem 277:29172-29180.
28. Richardson JS, Richardson DC. 1988. Amino acid preferences for specific locations at the ends of oc-helices. Science 240:1648-1652.
29. Runswich MJ, Walker JE, Bisaccia F, Iacobazzi V, Palmieri F. 1990. Sequence of the bovine 2-oxoglutarate/malate carrier protein: Structural relationship to other mitochondrial transport proteins. Biochemistry 29:11033-11040.
30. Sreerama N, Woody RW 2000. Circular dichroism of peptides and proteins. In: Berova N, Nakanishi K, Woody RW, editors. Circular dichroism: principles and applications. New York: Wiley. p 601-620.
31. States DJ, Haberkorn RA, Ruben DJ. 1982. A two-dimensional nuclear Overhauser experiment with pure absorption phase in four quadrants. J Magn Reson 48:286-292.
32. Stipani V, Cappello AR, Daddabbo L, Natuzzi D, Miniero DV, Stipani I, Palmieri F. 2001. The mitochondrial oxoglutarate carrier: cysteine-scanning mutagenesis of transmembrane domain IV and sensitivity of Cys mutants to sulfhydryl reagents. Biochemistry 40:15805-15810.
33. von Heijne G. 1991. Proline kinks in transmembrane α-helices. J Mol Biol 218:499-503.
34. Walker JE, Runswick MJ. 1993. The mitochondrial protein superfamily. J Bioenerg Biomembr 25:435-446.
35. Wishart DS, Sykes BD, Richards FM. 1991. Relationship between nuclear magnetic resonance chemical shift and protein secondary structure. J Mol Biol 222:311-333.
36. Wüthrich K, Billeter M, Braun W. 1983. Pseudo-structures for the 20 common amino acids for use in studies of protein conformations by measurements of intramolecular proton-proton distance constraints with nuclear magnetic resonance. J Mol Biol 169:949-961.
37. Wuthrich K. 1986. NMR of proteins and nucleic acids. New York: Wiley.