Identification of mutation sites on Δ6 desaturase genes from Mortierella alpina 1S-4 mutants

Bioscience, Biotechnology and Biochemistry

Volumn 69, Issue 5, 2005, Pages 1021-1024

  Abe, Takahiro ^a   Sakuradani, Eiji ^a   Asano, Takahiro ^a   Kanamaru, Hiroyuki ^a   Ioka, Yuji ^a   Shimizu, Sakayu ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

6 desaturase; Linoleic acid; Mortierella alpina; Mutant; Polyunsaturated fatty acid

Indexed keywords

AMINO ACIDS; BIOCHEMISTRY; BIOTECHNOLOGY; ENZYMES; MUTAGENESIS;

DESATURATION; INTRONS; MUTANTS; MUTATION;

GENES;

5,11,14 ICOSATRIENOIC ACID; ACYL COENZYME A DESATURASE; ARACHIDONIC ACID DERIVATIVE; EICOSA-5,11,14-TRIENOIC ACID;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; GENETICS; MOLECULAR GENETICS; MORTIERELLA; MUTATION; NUCLEOTIDE SEQUENCE;

AMINO ACID SEQUENCE; ARACHIDONIC ACIDS; BASE SEQUENCE; FATTY ACID DESATURASES; MOLECULAR SEQUENCE DATA; MORTIERELLA; MUTATION;

MORTIERELLA ALPINA;

EID: 22444437949     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.69.1021     Document Type: Article

References (10)

1. Jareonkitmongkol, S., Shimizu, S., and Yamada, H., Fatty acid desaturation-defective mutants of an arachidonic-acid-producing fungus, Mortierella alpina 1S-4. J. Gen. Microbiol., 138, 997-1002 (1992).
2. Jareonkitmongkol, S., Sakuradani, E., and Shimizu, S., A novel Δ5 desaturase-defective mutant of Mortierella alpina 1S-4 and its dihomo-γ-linolenic acid productivity. Appl. Environ. Microbiol., 59, 4300-4304 (1993).
3. Sakuradani, E., Kamada, N., Hirano, Y., Nishihara, M., Kawashima, H., Akimoto, K., Higashiyama, K., Ogawa, J., and Shimizu, S., Production of 5,8,11-eicosatrienoic acid by a Δ5 and Δ6 desaturation activity-enhanced mutant derived from a Δ12 desaturation activity-defective mutant of Mortierella alpina 1S-4. Appl. Microbiol. Biotechnol., 60, 281-287 (2002).
4. Jareonkitmongkol, S., Shimizu, S., and Yamada, H., Occurrence of two nonmethylene interrupted fatty acids in a Δ5 polyunsaturated fatty acid in a Δ6 desaturase-defective mutant of the fungus Mortierella alpina 1S-4. Biochim. Biophys. Acta, 1167, 137-141 (1993).
5. Takagi, T., 5,11,14-eicosatrienoic acid in Podocarpus nagi seed oil. J. Am. Oil Chem. Soc., 41, 516-519 (1964).
6. Berger, A., Monnard, I., Baur, M., Charbonnet, C., Safonova, I., and Jomard, A., Epidermal anti-inflammatory properties of 5,11,14 20:3: Effects on mouse ear edema, PGE2 levels in cultured keratinocytes, and PPAR activation. Lipids Health Dis., 1, 1-12 (2002).
7. Sakuradani, E., Kobayashi, M., and Shimizu, S., Δ6-Fatty acid desaturase from an arachidonic acid-producing Mortierella fungus: Gene cloning and its heterologous expression in a fungus, Aspergillus. Gene, 238, 445-453 (1999).
8. Sakuradani, E., and Shimizu, S., Gene cloning and functional analysis of a second Δ6-Fatty acid desaturase from an arachidonic acid-producing Mortierella fungus. Biosci. Biotechnol. Biochem., 67, 704-711 (2003).
9. 9-fatty acid desaturase from arachidonic acid-producing fungus: Unique gene sequence and its heterologous expression in a fungus, Aspergillus. Eur. J. Biochem., 260, 208-216 (1999).
10. Shanklin, J., Whittle, E., and Fox, B. G., Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase. Biochemistry, 33, 12787-12794 (1994).