Structural characterization of monomeric folding intermediates of recombinant human macrophage-colony stimulating factor β (rhM-CSFβ) by chemical trapping, chromatographic separation and mass spectrometric peptide mapping

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 782, Issue 1-2, 2002, Pages 393-404

  Happersberger, H Peter ^a,c   Cowgill, Cynthia ^b   Glocker, Michael O ^a  

^a UNIVERSITY OF ROSTOCK   (Germany)

^b NOVARTIS PHARMA AG   (Switzerland)

^c Scil Technology GmbH   (Germany)

Author keywords

Monomeric folding intermediates; Peptide mapping; Recombinant human macrophage colony stimulating factor

Indexed keywords

DIMERS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MONOMERS; SEPARATION;

RING STRUCTURES;

PROTEINS;

COLONY STIMULATING FACTOR 1; DIMER; MELARSENOXIDE; MONOMER; RECOMBINANT PROTEIN;

ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PEPTIDE MAPPING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FOLDING; PROTEIN MODIFICATION; SEPARATION TECHNIQUE; CHEMICAL STRUCTURE; CHEMISTRY; GEL CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION;

CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; MACROPHAGE COLONY-STIMULATING FACTOR; MASS SPECTROMETRY; MODELS, MOLECULAR; PEPTIDE MAPPING; PROTEIN CONFORMATION; RECOMBINANT PROTEINS;

EID: 2242456808     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1570-0232(02)00555-X     Document Type: Article

References (24)

1. Przybylski M., Glocker M. Angew. Chem. Intl. Ed. Engl. 35/8:1996;806.
2. Chaurand P., Luetzenkirchen F., Spengler B. J. Am. Soc. Mass Spectrom. 10:1999;91.
3. Happersberger H.P., Glocker M.O. Eur. Mass Spectrom. 4:1998;209.
4. Happersberger H.P., Przybylski M., Glocker M.O. Anal. Biochem. 264:1998;237.
5. Li Y.J., Rothwarf D.M., Scheraga H.A. Nature Struct. Biol. 2:1995;489.
6. Creighton T.E. Biol. Chem. 378:1997;731.
7. Hoffman M. Science. 235:1991;1357.
8. Creighton T.E. Science. 256:1992;111.
9. Weissman J.S., Kim P.S. Science. 256:1992;112.
10. Happersberger H.P., Stapleton J., Cowgill C., Glocker M.O. Proceedings of the 45th Conference on Mass Spectrometry an Allied Topics:1997;22.
11. Aukerman S.L., Middleton S., Sampson-Johannes A., Ralph P. Symann M., Quesenberry P., Morstyn G. Hematopoietic Growth Factors and Interleukins: Biology and Clinical Applications. 1992;79 Gardiner-Caldwell, Macclesfield.
12. Clark S.C., Kamen R. Science. 236:1987;1229.
13. Stanley E.R. Nicola N.A. Guidebook To Cytokines and Their Receptors. 1994;164.
14. Halenbeck R., Kawasaki E., Wrin J., Koths K. Bio/Technology. 7:1989;710.
15. Glocker M.O., Arbogast B., Schreurs J., Deinzer M.L. Biochemistry. 32:1993;482.
16. Pandit J., Bohm A., Jancarik J., Halenbeck R., Koths K., Kim S.-H. Science. 258:1992;1358.
17. Glocker M.O., Arbogast B., Milley R., Cowgill C., Deinzer M.L. Proc. Natl. Acad. Sci. USA. 91:1994;5868.
18. Wilkins J.A., Cone J., Randhawa Z.I., Wood D., Warren M.K., Witkowska H.E. Protein Sci. 2:1993;244.
19. Happersberger H.P., Stapleton J., Cowgill C., Glocker M.O. Proteins Suppl. 2:1998;50.
20. Schägger H., Jagow G.v. Anal. Biochem. 173:1988;201.
21. Fligge T.A., Przybylski M., Quinn J.P., Marshall A.G. Eur. Mass Spectrom. 4:1998;401.
22. Jauhiainen M., Stevenson K.J., Dolphin P.J. J. Biol. Chem. 263:1988;6525.
23. Dill K.A. Biochemistry. 29:1990;7133.
24. Roepstorff P., Fohlman J. Biomed. Mass Spectrom. 11:1984;601.