Serpins in fruit and vegetative tissues of apple (Malus domestica): Expression of four serpins with distinct reactive centres and characterisation of a major inhibitory seed form, MdZ1b

Functional Plant Biology

Volumn 32, Issue 6, 2005, Pages 517-527

  Hejgaard, Jørn ^a   Laing, William A ^b   Marttila, Salla ^c   Gleave, Andrew P ^b   Roberts, Thomas H ^d  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b THE NEW ZEALAND INSTITUTE FOR PLANT AND FOOD RESEARCH LIMITED   (New Zealand)

^c SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^d MACQUARIE UNIVERSITY   (Australia)

Author keywords

Apple; EST; Proteinase inhibitor; Seed; Serpin; Tissue expression

Indexed keywords

ENZYME INHIBITION; ENZYME KINETICS; ENZYMES; GENES; GRAIN (AGRICULTURAL PRODUCT); SEED; TISSUE; VEGETATION;

CHYMOTRYPSIN; PROTEINASES; REACTIVE CENTER LOOP (RCP); SERPINS;

FRUITS;

FRUIT; INHIBITION;

APPLES; CEREALS; ENZYMES; GENES; PLANT TISSUES; PLANTS; SEEDS; VARIETIES;

BOVINAE; MALUS X DOMESTICA; SUIDAE;

EID: 22344434369     PISSN: 14454408     EISSN: None     Source Type: Journal    
DOI: 10.1071/FP04220     Document Type: Article

References (27)

1. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25, 3389-3402. doi: 10.1093/nar/25.17. 3389
2. Beatty K, Bieth J, Travis J (1980) Kinetics of association of serine proteinases with native and oxidized alpha-1-proteinase inhibitor and alpha-1-antichymotrypsin. Journal of Biological Chemistry 255, 3931-3934.
3. Brandt A, Svendsen I, Hejgaard J (1990) A plant serpin gene. Structure, organization and expression of the gene encoding barley protein Z4. European Journal of Biochemistry 194, 499-505. doi: 10.1111/j.1432-1033.1990.tbl5644.x
4. Calugaru SV, Swanson R, Olson ST (2001) The pH dependence of serpin-proteinase complex dissociation reveals a mechanism of complex stabilization involving inactive and active conformational states of the proteinase which are perturbable by calcium. Journal of Biological Chemistry 276, 32446-32455. doi: 10.1074/jbc.M104731200
5. Dahl SW, Rasmussen SK, Hejgaard J (1996a) Heterologous expression of three plant serpins with distinct inhibitory specificities. Journal of Biological Chemistry 271, 25083-25088. doi: 10.1074/jbc.271.41.25083
6. Dahl SW, Rasmussen SK, Petersen LC, Hejgaard J (1996b) Inhibition of coagulation factors by recombinant barley serpin BSZx. FEBS Letters 394, 165-168. doi: 10.1016/0014-5793(96)00940-4
7. Gettins PG (2002) Serpin structure, mechanism, and function. Chemical Reviews 102, 4751-4804. doi: 10.1021/cr010170+
8. Giese H, Hejgaard J (1984) Synthesis of salt-soluble proteins in barley. Pulse-labeling study of grain filling in liquid-cultured detached spikes. Planta 161, 172-177. doi: 10.1007/BF00395478
9. Hejgaard J (2001) Inhibitory serpins from rye grain with glutamine as P1 and P2 residues in the reactive center. FEBS Letters 488, 149-153. doi: 10.1016/S0014-5793(00)02425-X
10. Hejgaard J, Hauge S (2002) Serpins of oat (Avena sativa) grain with distinct reactive centres and inhibitory specificity. Physiologia Plantarum 116, 155-163. doi: 10.1034/j.1399-3054.2002.1160204.x
11. Huntington JA, Read RJ, Carrell RW (2000) Structure of a serpin-protease complex shows inhibition by deformation. Nature 407, 923-926. doi: 10.1038/35038119
12. Keates SE, Kostman TA, Anderson JD, Bailey BA (2003) Altered gene expression in three plant species in response to treatment with Nep1, a fungal protein that causes necrosis. Plant Physiology 132, 1610-1622. doi: 10.1104/pp.102.019836
13. Koiwa H, Bressan RA, Hasegawa PM (1997) Regulation of protease inhibitors and plant defense. Trends in Plant Science 1, 379-384. doi: 10.1016/S1360-1385(97)90052-2
14. Laing WA, McManus MT (2002) Proteinase inhibitors. In 'Protein-protein interactions in plants'. (Eds MT McManus, WA Laing, AC Allan) pp. 77-119. (Sheffield Academic Press: Sheffield)
15. Mundy J, Svendsen I, Hejgaard J (1983) Barley alpha-amylase/subtilisin inhibitor. I. Isolation and characterization. Carlsberg Research Communications 48, 81-90.
16. Ohkubo K, Ogata S, Misumi Y, Takami N, Ikehara Y (1991) Molecular cloning and characterization of rat contrapsin-like protease inhibitor and related proteins. Journal of Biochemistry 109, 243-250.
17. Østergaard H, Rasmussen SK, Roberts TH, Hejgaard J (2000) Inhibitory serpins from wheat grain with reactive centers resembling glutamine-rich repeats of prolamin storage proteins. Cloning and characterization of five molecular forms. Journal of Biological Chemistry 275, 33272-33279. doi: 10.1074/jbc.M004633200
18. Roberts TH, Marttila S, Rasmussen SK, Hejgaard J (2003) Differential gene expression for suicide-substrate serine proteinase inhibitors (serpins) in vegetative and grain tissues of barley. Journal of Experimental Botany 54, 2251-2263. doi: 10.1093/jxb/erg248
19. Rosenkrands I, Hejgaard J, Rasmussen SK, Bjorn SE (1994) Serpins from wheat grain. FEBS Letters 343, 75-80. doi: 10.1016/0014-5793(94)80610-1
20. Ryan SN, McManus MT, Laing WA (2003) Identification and characterisation of proteinase inhibitors and their genes from seeds of apple (Malus domestica). Journal of Biochemistry 134, 31-42. doi: 10.1093/jb/mvg110
21. Shatters Jr RG, Bausher MG, Hunter WB, Chaparro JX, Dang PM, Niedz RP, Mayer RT, McCollum TG, Sinisterra X (2004) Putative protease inhibitor gene discovery and transcript profiling during fruit development and leaf damage in grapefruit (Citrus paradisi Macf). Gene 326, 77-86. doi: 10.1016/j.gene.2003.10. 010
22. Shewry PR (1999) Enzyme inhibitors of seeds: types and properties. In 'Seed proteins'. (Eds PR Shewry, R Casey) pp. 587-615. (Kluwer Academic Publishers: Dordrecht)
23. Sokal R, Rohlf FJ (1995) 'Biometry. 3rd edn.' (WH Freeman and Company: San Francisco)
24. Thomas JC, Adams DG, Keppenne VD, Wasmann CC, Brown JK, Kanost MR, Bohnert HJ (1995) Manduca sexta encoded protease inhibitors expressed in Nicotiana tabacum provide protection against insects. Plant Physiology and Biochemistry 33, 611-614.
25. Walz C, Giavalisco P, Schad M, Juenger M, Klose J, Kehr J (2004) Proteomics of curcurbit phloem exudate reveals a network of defence proteins. Phytochemistry 65, 1795-1804. doi: 10.1016/j.phytochem.2004.04.006
26. Westwood MN (1978) 'Temperate-zone pomology.' (WH Freeman and Company: San Francisco)
27. Yoo B-C, Aoki K, Xiang Y, Campbell LR, Hull RJ, Xoconostle-Cázares B, Monzer J, Lee J-Y, Ullman DE, Lucas WJ (2000) Characterization of Cucurbita maxima phloem Serpin-1 (CmPS-1): a developmentally regulated elastase inhibitor. Journal of Biological Chemistry 275, 35122-35128. doi: 10.1074/jbc.M006060200