HPLC purification of recombinant NcGRA6 antigen improves enzyme-linked immunosorbent assay for serodiagnosis of bovine neosporosis

Veterinary Parasitology

Volumn 131, Issue 3-4, 2005, Pages 227-234

  Jenkins, M C ^a   Fetterer, R ^a   Schares, G ^b   Bjorkman C ^c   Wapenaar, W ^d   McAllister, M ^e   Dubey, J P ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

^c SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^d UNIVERSITY OF PRINCE EDWARD ISLAND   (Canada)

^e UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

Cattle; Neospora caninum; Recombinant protein; Serological assays

Indexed keywords

NEOSPORA CANINUM RECOMBINANT DENSE GRANULE PROTEIN 6; PARASITE ANTIGEN; RECOMBINANT PROTEIN; SILVER; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANTIGEN BINDING; ANTIGEN DETECTION; ANTIGEN PURIFICATION; ARTICLE; BEEF CATTLE; BOVINE NEOSPOROSIS; CATTLE DISEASE; CONTROLLED STUDY; COW; DEVELOPMENTAL STAGE; ELUTION; ENZYME LINKED IMMUNOSORBENT ASSAY; EPIDEMIC; ESCHERICHIA COLI; HERD; IMMUNOASSAY; IMMUNOBLOTTING; IMMUNOFLUORESCENCE TEST; INTERMETHOD COMPARISON; NEOSPORA CANINUM; NONHUMAN; PARASITOSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PURIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SENSITIVITY AND SPECIFICITY; SEROLOGY; STAINING; STATISTICAL SIGNIFICANCE;

ANIMALS; ANTIBODIES, PROTOZOAN; ANTIGENS, PROTOZOAN; CATTLE; CATTLE DISEASES; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COCCIDIOSIS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE, DIRECT; IMMUNOBLOTTING; ISCOMS; NEOSPORA; RECOMBINANT PROTEINS; ROC CURVE;

BOS TAURUS; BOVINAE; ESCHERICHIA COLI; NEOSPORA CANINUM;

EID: 22244459493     PISSN: 03044017     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetpar.2005.05.005     Document Type: Article

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