An examination of non-formalin-based fixation methods for Xenopus embryos

Developmental Dynamics

Volumn 233, Issue 4, 2005, Pages 1464-1469

  Acton, Anthony ^a   Harvey, Tia ^a   Grow, Matthew W ^a,b  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b BRTC   (United States)

Author keywords

Antibody; Embryo; FineFIX; Fixation; Fixative; In situ hybridization; MEMFA; MEMPFA; Non formalin; NOTOXhisto; Paraformaldehyde; Whole mount immunohistochemistry; Xenopus

Indexed keywords

FORMALDEHYDE; PARAFORMALDEHYDE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CROSS LINKING; DIAGNOSTIC KIT; EMBRYO; FLUORESCENCE; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; NONHUMAN; PRIORITY JOURNAL; TISSUE FIXATION; XENOPUS LAEVIS;

ANIMALS; EMBRYO, NONMAMMALIAN; ENDOPEPTIDASE K; FORMALDEHYDE; GENES, REPORTER; HOMEODOMAIN PROTEINS; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; POLYMERS; TISSUE FIXATION; TRANSCRIPTION FACTORS; TROPONIN I; XENOPUS LAEVIS; XENOPUS PROTEINS;

XENOPUS LAEVIS;

EID: 22244447717     PISSN: 10588388     EISSN: None     Source Type: Journal    
DOI: 10.1002/dvdy.20448     Document Type: Article

References (10)

1. Drysdale TA, Tonissen KF, Patterson KD, Crawford MJ, Krieg PA. 1994. Cardiac troponin I is a heart-specific marker in the Xenopus embryo: expression during abnormal heart morphogenesis. Dev Biol 165:432-441.
2. French D, Edsall JT. 1945. The reaction of formaldehyde with amino acids and proteins. Adv Protein Chem 2:227-335.
3. Harland RM. 1991. In situ hybridization: an improved whole-mount method for Xenopus embryos. Methods Cell Biol 36: 685-695.
4. Heim R, Cubitt AB, Tsien RY. 1995. Improved green fluorescence. Nature 373: 663-664.
5. Kintner CR, Brockes JP. 1984. Monoclonal antibodies identify blastemal cells derived from dedifferentiating limb regeneration. Nature 308:67-69.
6. Nieuwkoop PD, Faber J. 1994. Normal table of Xenopus laevis (Daudin). A systematical and chronological survey of the development from the fertilized egg till the end of metamorphosis. New York: Garland Publishing, Inc. 260 p.
7. Prento P, Lyon H. 1997. Commercial formalin substitutes for histopathology. Biotech Histochem 72:273-282.
8. Sive H, Grainger RM, Harland RM. 2000. Early development of Xenopus laevis: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. 338 p.
9. Tonissen KF, Drysdale TA, Lints TJ, Harvey RP, Krieg PA. 1994. XNkx-2.5, a Xenopus gene related to Nkx-2.5 and tinman: evidence for & conserved role in cardiac development. Dev Biol 162:325-328.
10. Werner M, Chott A, Fabiano A, Battifora H. 2000. Effect of formalin tissue fixation and processing on immunohistochemistry. Am J Surg Pathol 24:1016-1019.