A fluorescence-based assay for fatty acid amide hydrolase compatible with high-throughput screening

Analytical Biochemistry

Volumn 343, Issue 1, 2005, Pages 143-151

  Ramarao, Manjunath K ^a   Murphy, Elizabeth A ^a   Shen, Marina W H ^a   Wang, Yuren ^a   Bushell, Kristen N ^a,b   Huang, Nelson ^a   Pan, Ning ^a   Williams, Cara ^a   Clark, James D ^a  

^a PFIZER INC   (United States)

^b BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Amidase; Anandamide; Enzymatic assay; Fatty acid amide hydrolase; Fluorescent assay; High throughput screening

Indexed keywords

AMIDES; CATALYSIS; CELL CULTURE; CELLS; FLUORESCENCE; HYDROLASES; HYDROLYSIS;

AMIDASE; ANANDAMIDE; ENZYMATIC ASSAY; FATTY ACID AMIDE HYDROLASE; FLUORESCENT ASSAYS; HIGH THROUGHPUT SCREENING;

FATTY ACIDS;

AMIDE; ARACHIDONIC ACID; ARACHIDONYL 7 AMINO 4 METHYLCOUMARINAMIDE; COUMARIN DERIVATIVE; FATTY ACID AMIDASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CATALYSIS; CHO CELL; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; FLUORESCENCE ANALYSIS; FLUOROMETRY; HIGH THROUGHPUT SCREENING; HYDROLYSIS; IC 50; INCUBATION TIME; MICROSOME; MOUSE; NONHUMAN; PRIORITY JOURNAL; RAT; STATISTICAL SIGNIFICANCE;

CRICETULUS GRISEUS;

EID: 22144485214     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.04.032     Document Type: Article

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