Quantitative estimation of channeling from early glycolytic intermediates to CO2 in intact Escherichia coli

FEBS Journal

Volumn 272, Issue 13, 2005, Pages 3260-3269

  Shearer, Georgia ^a   Lee, Jennifer C ^a   Koo, Jia An ^a   Kohl, Daniel H ^a  

^a WASHINGTON UNIVERSITY   (United States)

Author keywords

Glycolysis; Metabolic channelling; Metabolon; Ratio of channeled flux to total flux

Indexed keywords

6 PHOSPHOFRUCTOKINASE; CARBON 14; CARBON DIOXIDE; CELL EXTRACT; ENZYME; FRUCTOSE 1,6 BISPHOSPHATE; FRUCTOSE 6 PHOSPHATE; GLUCOSE; GLUCOSE 6 PHOSPHATE; GLUCOSE 6 PHOSPHATE ISOMERASE; GLYCEROL; WATER;

ARTICLE; BACTERIAL GROWTH; CHANNELING; CONTROLLED STUDY; CYTOSOL; ESCHERICHIA COLI; GLYCOLYSIS; IN VIVO STUDY; ISOTOPE DILUTION ASSAY; METABOLIC REGULATION; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS;

CARBON DIOXIDE; CARBON RADIOISOTOPES; CATALYSIS; ESCHERICHIA COLI; FRUCTOSEDIPHOSPHATES; FRUCTOSEPHOSPHATES; GLUCOSE; GLUCOSE-6-PHOSPHATE; GLYCOLYSIS; KINETICS; PENTOSE PHOSPHATE PATHWAY; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI;

EID: 22144471210     PISSN: 1742464X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2005.04712.x     Document Type: Article

References (18)

1. Agius L & Sherratt H, eds. (1997) Channelling in Intermediary Metabolism. Portland Press, London.
2. Ovadi J & Srere P (2000) Macromolecular compartmentation and channelling. Int Rev Cytol 192, 255-280.
3. Velot C & Srere P (2000) Reversible transdominant inhibition of a metabolic pathway. J Biol Chem 275, 12926-12933.
4. Srere P (1987) Complexes of sequential metabolic enzymes. Annu Rev Biochem 56, 89-124.
5. Milani M, Pesce A, Bolognesi M, Bocedi A & Ascenzi P (2003) Substrate channeling: molecular bases. Biochem Mol Biol Educ 31, 228-233.
6. Gutfreund H & Chock P (1991) Substrate channeling among glycolytic enzymes - fact or fiction. J Theor Biol 152, 117-121.
7. Atkinson D (1977) Conservation of solvent capacity. In Cellular Energy Metabolism and its Regulation, pp. 13-31. Academic Press, New York.
8. Masters CJ (1977) Metabolic control and the microenvironment. Curr Topics Cell Regul 12, 75-105.
9. Beeckmans S (1999) Chromatographic methods to study protein-protein interactions. Methods 19, 278-305.
10. Law RD & Plaxton WC (1997) Regulatory phosphorylation of banana fruit phosphoenolpyruvate carboxylase by a copurifying phosphoenolpyruvate carboxylase-kinase. Eur J Biochem 247, 642-651.
11. Datta A, Merz J & Spivey H (1985) Substrate channeling of oxalacetate in solid-state complexes of malate dehydrogenase and citrate synthase. J Biol Chem 260, 15008-15012.
12. Debnam P, Shearer G, Blackwood L & Kohl DH (1997) Evidence for channelling of intermediates in the oxidative limb of the pentose phosphate pathway by soybean and pea nodule extracts, yeast extracts and purified yeast enzymes. Eur J Biochem 246, 283-290.
13. Cohen NS & Kuda A (1996) Argininosuccinate synthetase and argininosuccinate lyase are localized around mitochondria: an immunocytochemical study. J Cell Biochem 60, 334-340.
14. Sumegi B, Sherry AD, Malloy CR & Srere PA (1993) Evidence for orientation-conserved transfer in the TCA cycle in Saccharomyces cerevisiae: carbon-13 NMR studies. Biochemistry 32, 12725-12729.
15. Haggie P & Brindle K (1999) Mitochondrial citrate synthase is immobilized in vivo. J Biol Chem 274, 3941-3945.
16. Clegg J & Jackson S (1990) Gucose metabolism and the channeling of glycolytic intermediates in permeabilized L-929 cells. Arch Biochem Biophys 278, 452-460.
17. Negrutskii BS & Deutscher MP (1991) Channeling of aminoacy-tRNA for protein synthesis in vivo. Proc Natl Acad Sci USA 88, 4991-4995.
18. Mowbray J & Moses V (1976) The tentative identification in Escherichia coli of a multienzyme complex with glycolytic activity. Eur J Biochem 66, 25-36.