Localization of epitopes in the toxins of Tityus serrulatus scorpions and neutralizing potential of therapeutic antivenoms

Toxicon

Volumn 46, Issue 2, 2005, Pages 210-217

  Maria, W S ^a   Velarde, D T ^a   Alvarenga, L M ^b   Nguyen, C ^c   Villard, S ^c   Granier, C ^c   Chavez Olortegui C ^b  

^a FUNDAÇÃO EZEQUIEL DIAS   (Brazil)

^b Federal Institute of Education   (Brazil)

^c Centre de Pharmacologie et Biotechnologie Pour la Santé   (France)

Author keywords

Antivenoms; Epitope mapping; Synthetic peptides; Tityus toxins

Indexed keywords

ANTIGEN; ANTISERUM; EPITOPE; PEPTIDE; TITYUS TOXIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; DRUG ACTIVITY; DRUG POTENCY; ENZYME LINKED IMMUNOSORBENT ASSAY; LINEAR SYSTEM; MICROTITER PLATE ASSAY; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; SCORPION;

AMINO ACID SEQUENCE; ANIMALS; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; HORSES; IMMUNE SERA; IMMUNOASSAY; MOLECULAR SEQUENCE DATA; NEUTRALIZATION TESTS; PEPTIDES; SCORPION VENOMS; SCORPIONS;

EQUUS CABALLUS; SCORPIONES; TITYUS; TITYUS SERRULATUS;

EID: 22044437989     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2005.03.024     Document Type: Article

References (23)

1. L.M. Alvarenga, C. Granier, C.R. Diniz, and C. Chávez- Olórtegui Induction of neutralizing antibodies against Tityus serrulatus scorpion toxins by immunization with a mixture of defined synthetic epitopes Toxicon 40 2002 89 95
2. G. Bechis, F. Sampieri, P.M. Yuan, T. Brando, M.F. Martin, C.R. Diniz, and H. Rochat Amino acid sequence of toxin VII, a β toxin from the venom of the scorpion Tityus serrulatus Biochem. Biophys. Res. Commun. 122 1984 1146 1153
3. J.A. Campos, O.S. Silva, M. Lopez, and L. Freire-Maia Signs, symptoms and treatment of severe scorpion poisoning in children D. Eaker T. Wadstrom Natural Toxins 1980 Pergamon Press Oxford 61 62
4. J.L. Cardoso, W.H.R. Fan, A.A. Araujo, M. Santalucia, and J.M. Lopes Óbitos por escorpionismo no Brasil (1990-1993) Rev. Soc. Bras. Med. Trop. 28 1990 10
5. C. Chavez-Olortegui, F. Molina, and C. Granier Molecular basis for the cross-reactivity of antibodies elicited by a natural anatoxin with α and β-toxins from the venom of Tityus serrulatus scorpion Mol. Immunol. 38 2002 867 876
6. C. Devaux, O. Clot-Faybesse, M. Juin, K. Mabrouk, J.M. Sabatier, and H. Rochat Monoclonal antibodies neutralizing the toxin II from Androctonus australis hector scorpion venom: usefulness of a synthetic, nontoxic analog FEBS Lett. 412 1997 456 460
7. C.R. Diniz Chemical and pharmacological aspects of Tityinae venom Bettin Arthropod Venoms 1978 Springer Berlin 379 394
8. R. Frank Spot-synthesis: an easy technique for the positionally addressable, parallel chemical synthesis on a membrane support Tetrahedron 48 1992 9217 9232
9. M. Goyffon, M. Vachon, and N. Broglio Epidemiology and clinical characteristics of scorpion envenomation in Tunisia Toxicon 20 1982 337 344
10. E. Jover, F. Couraud, and H. Rochat Two types of scorpion neurotoxins characterized by their binding to separate receptor sites on rat brain synaptossomes Biochem. Biophys. Res. Commun. 95 1980 1607 1614
11. 50 J.H. Burn Biological Standartization 1937 Oxford University Press London 37 40
12. D. Laune, F. Molina, G. Ferrières, S. Villard, C. Bès, F. Rieunier, T. Chardes, and C. Granier Application of the Spot method to the identification of peptides and amino acids from the antibody paratope that contribute to antigen binding J. Immunol. Methods 267 2002 53 70
13. P. Mansuelle, M.F. Martin-Eauclaire, C. Chávez-Olórtegui, M.H. De Lima, H. Rochat, and C. Granier The β-type toxin TsII from the scorpion Tityus serrulatus: amino acid sequence determination and asessment of biological and antigenic properties Nat. Toxins 1 1992 119 125
14. W.S. Maria, M.O. Cambuy, J.O. Costa, D.T. Velarde, and C. Chávez-Olórtegui Neutralizing potency of horse antibothropic antivenom correlation between in vivo and in vitro methods Toxicon 36 1998 1433 1439
15. W.S. Maria, B. Pacheco, C. Barbosa, D.T. Velarde, and C. Chávez-Olórtegui Determination of the neutralizing potency of horse antibothropic and anticrotalic antivenoms in blood samples collected on filter paper Toxicon 39 2001 1607 1609
16. M.F. Martin-Eauclaire, B. Céard, A.M. Ribeiro, C.R. Diniz, H. Rochat, and P.E. Bougis Biochemical, pharmacological and genomic characterization of TsIV, an α-toxin from the venom of the South American scorpion Tityus serrulatus FEBS Lett. 342 1994 181 184
17. F. Molina, D. Laune, C. Gougat, B. Pau, and C. Granier Improved performances of Spot multiple peptide synthesis Peptide Res. 9 1996 151 155
18. L.D. Possani, B.M. Martin, J. Mochca-Morales, and I. Svendsen Purification and chemical characterization of the major toxins from the venom of the Brazilian scorpion Tityus serrulatus Carlsberg Res. Commun. 46 1981 195 205
19. N. Rezende, M. Bordes Dias, D. Campolina, C. Chávez- Olórtegui, C.R. Diniz, and C. Amaral Efficacy of antivenom therapy for neutralizing circulating venom antigens in patients stung by Tityus serrulatus scorpions Am. J. Trop. Med. Hyg. 52 1995 277 280
20. J. Rungsiwongse, and K. Ratanabanangkoon Development of an ELISA to assess the potency of horse therapeutic antivenom against Thai cobra venom J. Immunol. Methods 136 1991 37 43
21. W. Russell, and R.L. Burch The Principles of Human Experimental Technique 1959 Tethuen London
22. R.D.G. Theakston, and H.A. Reid Enzyme-Linked Immunosorbent Assay (ELISA) in assessing antivenom potency Toxicon 17 1979 511 515
23. Word Health Organization,1981. Progress in the characterization of venoms and standardization of antivenoms. WHO Offset Publication No 58 Geneva: World Health Organization.