Mechanism and progress of (iso)thiocyanate approach for C-terminal sequence analysis of proteins

Progress in Biochemistry and Biophysics

Volumn 26, Issue 3, 1999, Pages 222-223

  Mo, Bi Lan ^a   Li, Jiang ^a   Liang, Song Ping ^a  

^a HUNAN NORMAL UNIVERSITY   (China)

Author keywords

(iso) thiocyanate approach; Activation; Amino acid thiohydantoins; Cleavage; Coupling; Cyclization; Protein C terminal sequencing

Indexed keywords

EID: 21944456733     PISSN: 10003282     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Edman P. Method for determination of the amino acid sequence in peptides. Acta Chem Scand, 1950, 4 (2): 283-293
2. (Tu H M, Xia Q C). (Prog Biochem Biophys), 1993, 20 (3): 181-185
3. Patterson D H, Tarr G E, Regnier F E, et al. C-terminal ladder sequencing via matrix-assisted laser desorption mass spectrometry coupled with carboxypeptidase Y time-dependent and concentration-dependent digestions. Anal Chem, 1995, 67 (21): 3971-3978
4. Bonetto V, Bergman A-C, Jornvall H, et al. C-terminal sequence determination of modified peptides by MALDI MS. J Protein Chem, 1997, 16 (5): 371-374
5. Schlack P, Kumpf W. Uber eine neue methode zur Ermittlung der konstitution von peptiden. Hoppe-Seyler's Z Physiol Chem, 1926, 154 (1): 125-170
6. Boyd V L, Bozzini M, Zon G, et al. Sequencing of peptides and proteins from the caboxy terminus. Anal Biochem, 1992, 206 (2): 344-352
7. Shenoy N R, Shively J E, Bailey J M. Studies in C-terminal sequencing: new reagents for the synthesis of peptidylthiohydantoins. J Protein Chem, 1993, 12 (2): 195-205
8. Wittmann-Liebold B, Matschull L, Pilling U, et al. Modular Berlin microsequencer for the sequential degradation of proteins and peptides from the amino-and carboxyl-terminal ends. In: Jornvall H, eds. Methods in Protein Sequence Analysis. Basel: Birkhauser Verlag, 1991. 9-21
9. Hawke K H, Boyd V L. Chemical C-tertninal sequencing. In: Jornvall H, eds. Methods in Protein Sequence Analysis. Basel: Birkhauser Verlag, 1991. 35-45
10. Bailey J M, Tu O, Issai G, et al. C-terminal sequence analysis of polypeptides containing C-terminal proline. In: Crabb J W ed. Techniques in Protein Chemistry VI. San Diego: Academic Press, 1995. 239-247
11. Bailey J M, Miller C G. Automated methods for C-terminal protein sequencing. In: Smith B J ed. Protein Sequencing Protocols. Totowa: Hunmana Press, 1997. 259-269
12. Miller C G, Hawke D H, Tso J, et al. Automated C-terminal protein sequence analysis using the Hwelett-Packard G1009A C-terminal protein sequencing system. In: Crabb J Wed. Techniques in Protein Chemistry VI. San Diego: Academic Press, 1995. 219 -227
13. Stark G R. Sequential degradation of peptides from their carboxyl termini with ammonium thiocyanate and acetic anhydride. Biochemistry, 1968, 7 (9): 1796-1807
14. Boyd V L, Bozzini M, Guga P J, et al. Activation of the carboxy terminus of a peptide for carboxy-terminal sequencing. J Org Chem, 1995, 60 (11): 2581-2587
15. Anumula K R, Tang S. Novel chemistry for sequencing of proteins from carboxyl terminus yields a simple method. FASEB J, 1995, 9 (6): 1275
16. Mo B L, Li J, Liang S P. Chemical carboxy-terminal sequence analysis of peptides using acetyl isothiocyanate. Anal Biochem, 1997, 252 (1): 169-176
17. Inglis A S, De Luca C. A new chemical approach to C-terminal microsequence analysis via the thiohydantoin. In: Imahori K, Sakiyama F eds. Methods in Protein Sequence Analysis. New York: Plenum Press, 1993. 71-78
18. Anumula K R, Tang S. Carboxy terminal sequencing of protein by a novel manual method. FASEB J, 1996, 10 (8): 2956
19. Boyd V L, Bozzini M, Zon G, et al. Sequencing of peptides and proteins from the carboxy terminus. Anal Biochem, 1992, 206 (2): 344-352
20. Bozzini M, Zhao J, Yuan P-M, et al. Applications using an alkylation method for carboxy-terminal protein sequencing. In: Crabb J W ed. Techniques in Protein Chemistry VI. San Diego: Academic Press, 1995. 229-237
21. Inglis A S. Chemical procedures for C-terminal sequencing of peptides and proteins. Anal Biochem, 1991, 195 (1): 183-196
22. Mo B L, Li J, Liang S P. A method for preparation of amino acid thiohydantoins from free amino acids activated by acetyl chloride for development of protein C-terminal sequencing. Anal Biochem, 1997, 249 (2): 207-211
23. (Mo B L, Li J, Liang S P). (Chin J Biochem Mol Biol), 1998, 14 (2): 186-191