Prokaryotic expression, purification and characterization of recombinant human protein kinase CK2β subunit

Progress in Biochemistry and Biophysics

Volumn 27, Issue 2, 2000, Pages 204-205

  Liu, Xin Guang ^a   Liang, Nian Ci ^a   Ma, Jian Quan ^b  

^a GUANGDONG MEDICAL UNIVERSITY   (China)

^b SUN YAT SEN UNIVERSITY   (China)

Author keywords

Enzyme kinetic analysis; Expression vector pT7 7; Human protein kinase CK2 subunit; Prokaryotic expression; Protein purification

Indexed keywords

EID: 21944449650     PISSN: 10003282     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Issinger O G. Casein kinase: pleiotropic mediators of cellular regulation. Pharmac Ther, 1993, 59 (1): 1-30
2. Allende J E, Allende C C. Protein kinase CK2: an enzyme with multiple substrates and a puzzling regulation. FASEB J, 1995, 9 (5): 313-323
3. Pinna L A, Meggio F. Protein kinase CK2 ("casein kinase-2") and its implication in cell division and proliferation. Prog Cell Cycle Res, 1997, 3: 77-97
4. Guerra B, Issinger O G. Protein kinase CK2 and its role in cellular proliferation, development and pathology. Electrophoresis, 1999, 20: 391-408
5. Liu X G, Liang N C, Ma J Q, et al. Chinese J of Biochem & Mol Biol, 1999, 15 (2): 228-231
6. Tabor S. Expression using the T7 RNA polymerase/promotor system. In; Ausubel F A, Brent R, Kingston R E, et al. Current Protocols in Molecular Biology. New York: Greene Publishing and Wiley-Interscience, 1990: 16.2.1-16.2.11
7. Nastainczyk W, Schmidt-Soaniol I, Boldyreff B, et al. Isolation and characterization of a monoclonal anti-protein kinase CK2β subunit antibody of the IgG class for the direct detection of CK2β-subunit in tissue cultures of various mammalian species and human tumors. Hybridoma, 1995, 14 (4): 335-339
8. Bradford M M. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 1976, 72 (1): 248-254
9. 2+-independent protein kinases. Methods Enzymatic Analysis. 1983, 3: 474-481
10. Marshak D R, Carroll D. Synthetic peptide substrates for casein kinase II. Methods in Enzymology. 1991, 200: 134-156
11. Liu X G, Liang N C, Ma J Q. Chinese J Biochem & Mol Biol, 2000, 21 (1): 17-22
12. Jakobi R, Traugh J A. Characterization of the phosphotransferase domain of casein kinase II by site-directed mutagenesis and expression in Escherichia coli. J Biol Chem, 1992, 267 (33): 23894-23902
13. Bodenbach L, Fauss J, Robitzki A, et al. Recombinant human casein kinase II. A study with the complete set of subunits (α, α′ and β), site-directed autophosphorylation mutants and a bicistronically expressed holoenzyme. Eur J Biochem, 1994, 220 (1): 263-273
14. Tuazon P T, Traugh J A. Casein kinase I and II: multipotential serine protein kinases: structure, function, and regulation. In: Greengard P, Robison G A, eds. Advances in Second Messenger and Phosphoprotein Research, 23. New York: Raven Press Ltd, 1991, 124-164