A new fluorescence resonance energy transfer pair and its application to oligonucleotide labeling and fluorescence resonance energy transfer hybridization studies

Journal of Fluorescence

Volumn 15, Issue 3, 2005, Pages 207-214

  Gruber, Michaela ^a,b   Wetzl, Bianca ^a   Oswald, Bernhard ^b   Enderlein, Joerg ^c   Wolfbeis, Otto S ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b Chromeon GmbH   (Germany)

^c FORSCHUNGSZENTRUM JÜLICH   (Germany)

Author keywords

Fluorescence hybridization assay; Fluorescence resonance energy transfer; Long wavelength fluorescent labels

Indexed keywords

AMINES; BIOASSAY; CHEMICAL MODIFICATION; DNA; DYES; ENERGY TRANSFER; ESTERS; MOLECULAR STRUCTURE; RESONANCE; SOLUBILITY;

FLUORESCENCE HYBRIDIZATION ASSAY; FLUORESCENCE RESONANCE ENERGY TRANSFER; LONG-WAVELENGTH FLUORESCENT LABELS; QUANTUM YIELDS (QY);

FLUORESCENCE;

EID: 21844446830     PISSN: 10530509     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10895-005-2619-y     Document Type: Article

References (15)

1. B. Oswald, M. Graber, M. Böhmer, F. Lehmann, M. Probst, and O. S. Wolfbeis (2001). Novel diode laser-compatible fluorophores and their application to single molecule detection, protein labeling and fluorescence resonance energy immunoassay. Photochem. Photobiol. 74, 237-245.
2. O. S. Wolfbeis, M. Böhmer, A. Dürkop, J. Enderlein, M. Gruber, I. Klimant, C. Krause, J. Kürner, G. Liebsch, Z. Lin, B. Oswald, and M. Wu (2002). In R. Kraayenhof (Ed.), Advanced Luminescent Labels, Probes, and Beads, and Their Application to Luminescence Bioassay, and Imaging in Springer Series in Fluorescence, vol. 2. Springer Verlag, Berlin-Heidelberg.
3. J. R. Lakowicz (1999). Principles of Fluorescence Spectroscopy, 2nd ed. Plenum, New York.
4. R. B. Mujumdar, L. A. Ernst, S. R. Mujumdar, C. J. Lewis, and A. S. Waggoner (1993). Cyanine dye labeling reagents: Sulfoindocyanine succinimidyl esters. Bioconjugate Chem. 4, 105-111.
5. F. M. Hamer (1949). Some unsymmetrical pentamethinecyanine dyes and their tetramethine intermediates. J. Chem. Soc. 32-37.
6. G. T. Hermanson (1996). Bioconjugate Techniques. Academic Press, San Diego, p. 13.
7. Oligonucleotide Phosphate Labeling Protocol, Molecular Probes (Eugene, Oregon), http://www.probes.com/handbook/sections/ 0802.html.
8. Fluoro Link Cy5, Product Description, Cat. No. PA25001. Amersham Life Science, Inc., IL.
9. J. N. Demas and G. A. Crosby (1971). The measurement of photoluminescence quantum yields. A review. J. Phys. Chem. 75, 991-1024.
10. S. Tyagi and F. R. Kramer (1996). Molecular beacons: Probes that fluoresce upon hybridization. Nat. Biotechnol. 14, 303-308.
11. S. A. E. Marras, F. R. Kramer, and S. Tyagi (2002). Efficiencies of fluorescence resonance energy transfer and contact-mediated quenching in oligonucleotide probes. Nucleic Acids Res. 30, e122-2 (electronic paper).
12. P. J. O'Sullivan, M. Burke, A. E. Soini, and D. B. Papkovsky (2002). Synthesis and evaluation of phosphorescent oligonucleotide probes for hybridisation assays. Nucleic Acids Res. 30, e114 (electronic paper).
13. S. Tyagi (2000). Wavelength-shifting molecular beacons. Nature Biotechnol. 18, 1191-1196.
14. M. Masuko, S. Ohuchi, K. Sode, H. Ohtani, and A. Shimadzu (2000). Fluorescence resonance energy transfer from pyrene to perylene labels for nucleic acid hybridization assays under homogeneous solution conditions. Nucleic Acids Res. 28, e34, 2-8.
15. R. Narayanaswamy and O. S. Wolfbeis (2003). Optical Sensors: Industrial, Environmental and Diagnostic Applications. Springer, Berlin, Heidelberg, chapters 4-6.