High-throughput flow cytometric screening of combinatorial chemistry bead libraries for proteomics and drug discovery

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 5692, Issue , 2005, Pages 216-223

  Leary, James F ^a   Reece, Lisa M ^a   Yang, Xianbin ^a   Gorenstein, David ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

Author keywords

Combinatorial libraries; Drug discovery; Flow Cytometry; MALDI TOF; NF B; Single bead analysis; Thioaptamers

Indexed keywords

COMBINATORIAL LIBRARIES; DRUG DISCOVERY; FLOW CYTOMETRY; MALDI-TOF; NF-ΚB; SINGLE-BEAD ANALYSIS; THIOAPTAMERS;

CAMERAS; DATA REDUCTION; DIGITAL DEVICES; DNA; DRUG DOSAGE; MASS SPECTROMETRY; RNA;

PROTEINS;

EID: 21844436805     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.589456     Document Type: Conference Paper

References (13)

1. Yang, X. and Gorenstein, D. G., Progress in thioaptamer development, Current Drug Targets, 5, 705-715, 2004.
2. Osborne, S. E. a. E., A.D., Nucleic acid selection and the challenge of combinatorial chemistry., Chem Rev., 97, 349-370, 1997.
3. Ludwig, L. a. E., F., Synthesis of nucleoside 5'-O-(1,3- dithiotriphosphates) and 5'-O-(1,1-dithiotriphosphates). J. Org. Chem., 56, 1777-1783, 1991.
4. Furka, A., Sebestyen, F., Asgedom, M. and Dibo, G., General method for rapid synthesis of multicomponent peptide mixtures, International Journal of Peptide & Protein Research, 37, 487-493, 1991.
5. Lam, K. S., Salmon, S.E., Hersh, E.M., Hruby, V.J., Kazmierskl, W.M. and Knapp, R., A new type of synthetic peptide library for identifying ligand-binding activity., Nature, 354, 82-84, 1991.
6. Yang, X., Bassett, S.E., Li, X., Luxon, B.A., Herzog, N.K., Shope, R.E., Aronson, J., Prow, T.W., Leary, J.F., Kirby, R., Ellington, A.D. and Gorenstein, D.G., Construction and selection of bead bound combinatorial oligonucleoside phosphorothioate and phosphorodithioate aptamer libraries designed for rapid PCR-based sequencing., Nucleic Acids research, 30, 2002.
7. Gosling, J. P., Immunoassays: A Practical Approach, Oxford University Press, Oxford, UK, 2000
8. Renard, P., Ernest, I., Houbio, A., Art, M., Calvez, H.L., Raes, M. and Remacle, J., Development of a sensitive multi-well colorimetric assay for NFκB., Nucleic Acids research, 29, 2001.
9. Yang, X., Lis, X., Prow, T.W., Reece, L.M., Bassett, S.E., Luxon, B.A., Herzog, N.K., Aronson, J., Shope, R.E., Leary, J.F., Gorenstein, D.G., Immunofluorescence assay and flow-cytometry selection of bead-bound aptamers, Nucleic Acids research, 31, 2003.
10. Corio, M. A., Leary, J.F., System for Flexibly Sorting Particles, 5,204,884, 1993.
11. Leary, J. F., Corio, M.A., McLaughlin, S.R., System for High-Speed Measurement and Sorting of Particles, 5,204,884, 1993.
12. Corio, M. A., Leary, J.F., System for Flexibly Sorting Particles, 5,550,058, 1996.
13. Leary, J. F., Corio, M.A., McLaughlin, S.R., System for High-Speed Measurement and Sorting of Particles, 5,804,143, 1998.