Rapid and quantitative detection of blood Serratia marcescens by a real-time PCR assay: Its clinical application and evaluation in a mouse infection model

FEMS Microbiology Letters

Volumn 248, Issue 2, 2005, Pages 163-170

  Iwaya, Akira ^a   Nakagawa, Saori ^a   Iwakura, Nobuhiro ^a   Taneike, Ikue ^a   Kurihara, Mizuki ^a   Kuwano, Tomoko ^a   Gondaira, Fumio ^a   Endo, Miyoko ^b   Hatakeyama, Katsuyoshi ^a   Yamamoto, Tatsuo ^a  

^a NIIGATA UNIVERSITY GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES   (Japan)

^b TOKYO METROPOLITAN INSTITUTE OF PUBLIC HEALTH   (Japan)

Author keywords

Clinical marker; Mouse infection; Nosocomial outbreaks; Real time PCR; Septicaemia; Serratia marcescens

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BLOOD ANALYSIS; COLONY FORMING UNIT; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DEATH; ENTEROBACTER INFECTION; FATALITY; MALE; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCALE UP; SERRATIA MARCESCENS; SURVIVAL RATE;

ANIMALS; CARBAPENEMS; DISEASE MODELS, ANIMAL; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; GENES, BACTERIAL; HUMANS; MALE; MICE; MICE, INBRED C57BL; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEPSIS; SERRATIA INFECTIONS; SERRATIA MARCESCENS;

SERRATIA MARCESCENS;

EID: 21744463047     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.femsle.2005.05.041     Document Type: Article

References (23)

1. M.B. Edmond, S.E. Wallace, D.K. McClish, M.A. Pfaller, R.N. Jones, and R.P. Wenzel Nosocomial bloodstream infections in United States hospitals: a three-year analysis Clin. Infect. Dis. 29 1999 239 244
2. J. Hebden, L. Fuss, R.S. Schwalbe, S. Qaiyumi, J.G. Morris Jr., and I.H. Gewolb Use of molecular epidemiology to investigate an outbreak of Serratia marcescens in a neonatal intensive care unit (NICU) Am. J. Infect. Control. 27 1999 184 185
3. J. Raymond, Y. Aujard European Study Group Nosocomial infections in pediatric patients: a European, multicenter prospective study Infect. Control Hosp. Epidemiol. 21 2000 260 263
4. L.H. Su, J.T. Ou, H.S. Leu, P.C. Chiang, Y.P. Chiu, J.H. Chia, A.J. Kuo, C.H. Chiu, C. Chu, T.L. Wu, C.F. Sun, T.V. Riley, B.J. Chang The Infection Control Group Extended epidemic of nosocomial urinary tract infections caused by Serratia marcescens J. Clin. Microbiol. 41 2003 4726 4732
5. P. Vigeant, V.G. Loo, C. Bertrand, C. Dixon, R. Hollis, M.A. Pfaller, A.P. McLean, D.J. Briedis, T.M. Perl, and H.G. Robson An outbreak of Serratia marcescens infections related to contaminated chlorhexidine Infect. Control Hosp. Epidemiol. 19 1998 791 794
6. M. McNaughton, N. Mazinke, and E. Thomas Newborn conjunctivitis associated with triclosan 0.5% antiseptic intrinsically contaminated with Serratia marcescens Can. J. Infect. Control. 10 1995 7 8
7. R.J. Körner, A. Nicol, D.S. Reeves, A.P. MacGowan, and J. Hows Ciprofloxacin resistant Serratia marcescens endocarditis as a complication of non-Hodgkin's lymphoma J. Infect. 29 1994 73 76
8. M. Zaidi, J. Sifuentes, M. Bobadilla, D. Moncada, and S. Ponce de León Epidemic of Serratia marcescens bacteremia and meningitis in a neonatal unit in Mexico City Infect. Control Hosp. Epidemiol. 10 1989 14 20
9. D.J. Passaro, L. Waring, R. Armstrong, F. Bolding, B. Bouvier, J. Rosenberg, A.W. Reingold, M. McQuitty, S.M. Philpott, W.R. Jarvis, S.B. Werner, L.S. Tompkins, and D.J. Vugia Postoperative Serratia marcescens wound infections traced to an out-of-hospital source J. Infect. Dis. 175 1997 992 995
10. W.W. Wong, L.S. Wang, D.L. Cheng, S.J. Lin, T.D. Chin, D.R. Hinthorn, M.C. O'Connor, and W.K. Huang Serratia marcescens bacteremia J. Formos. Med. Assoc. 90 1991 88 93
11. W.L. Yu, C.W. Lin, and D.Y. Wang Serratia marcescens bacteremia: clinical features and antimicrobial susceptibilities of the isolates J. Microbiol. Immunol. Infect. 31 1998 171 179
12. Bureau of Public Health Tokyo Metropolitan Government, Tokyo (2000) Report of the study group for disease of unknown cause (in Japanese).
13. Sakai city Government Public Health and Welfare Bureau (2000) The report of nosocomial Serratia marcescens outbreak in Sakai city (in Japanese).
14. Setagaya Ward, Tokyo (2002) Report of measures against Serratia marcescens nosocomial infection incidents (in Japanese).
15. K. Sen Rapid identification of Yersinia enterocolitica in blood by the 5′ nuclease PCR assay J. Clin. Microbiol. 38 2000 1953 1958
16. C.E. Corless, M. Guiver, R. Borrow, V. Edwards-Jones, A.J. Fox, and E.B. Kaczmarski Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCR J. Clin. Microbiol. 39 2001 1553 1558
17. K. Sen, and D.M. Asher Multiplex PCR for detection of Enterobacteriaceae in blood Transfusion 41 2001 1356 1364
18. T.Y. Tan, S. Corden, R. Barnes, and B. Cookson Rapid identification of methicillin-resistant Staphylococcus aureus from positive blood cultures by real-time fluorescence PCR J. Clin. Microbiol. 39 2001 4529 4531
19. M.L. Tondella, D.F. Talkington, B.P. Holloway, S.F. Dowell, K. Cowley, M. Soriano-Gabarro, M.S. Elkind, and B.S. Fields Development and evaluation of real-time PCR-based fluorescence assays for detection of Chlamydia pneumoniae J. Clin. Microbiol. 40 2002 575 583
20. Z.Y. Shi, P.Y. Liu, Y.J. Lau, Y.H. Lin, and B.S. Hu Use of pulsed-field gel electrophoresis to investigate an outbreak of Serratia marcescens J. Clin. Microbiol. 35 1997 325 327
21. I. Taneike, S. Nakagawa, A. Iwaya, and T. Yamamoto Nosocomial outbreak-derived Serratia marcescens: in vitro susceptibility to antimicrobial agents in comparison with carbapenem-resistant strains Acta Med. Biol. 50 2002 177 183
22. Y. Arakawa, N. Shibata, K. Shibayama, H. Kurokawa, T. Yagi, H. Fujiwara, and M. Goto Convenient test for screening metallo-β-lactamase-producing gram-negative bacteria by using thiol compounds J. Clin. Microbiol. 38 2000 40 43
23. N. Shibata, Y. Doi, K. Yamane, T. Yagi, H. Kurokawa, K. Shibayama, H. Kato, K. Kai, and Y. Arakawa PCR typing of genetic determinants for metallo-β-lactamases and integrases carried by gram-negative bacteria isolated in Japan, with focus on the class 3 integron J. Clin. Microbiol. 41 2003 5407 5413