Determination of amino acids in cell culture and fermentation broth media using anion-exchange chromatography with integrated pulsed amperometric detection

Journal of Biomolecular Techniques

Volumn 15, Issue 4, 2004, Pages 317-324

  Hanko, Valoran P ^a   Heckenberg, Andrea ^a   Rohrer, Jeffrey S ^a  

^a DIONEX CORPORATION   (United States)

Author keywords

Amino acids; Amperometry; Carbohydrates; Chromatography; Media

Indexed keywords

ACETIC ACID DERIVATIVE; AMINO ACID; CARBOHYDRATE; LYSINE; SUGAR ALCOHOL;

ARTICLE; CULTURE MEDIUM; FERMENTATION; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; TIME;

ACETATES; AMINO ACIDS; CARBOHYDRATES; CHROMATOGRAPHY, ION EXCHANGE; CULTURE MEDIA; FERMENTATION; LYSINE; SUGAR ALCOHOLS; TIME FACTORS;

EID: 21644481102     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (4)

1. Dionex Corporation. Determination of amino acids in cell cultures and fermentation broths. Application Note 150, LPN 1538. July 2003.
2. Hanko VP, Rohrer JS. Determination of amino acids in cell culture and fermentation broth media using anionexchange chromatography with integrated pulsed amperometric detection. Anal Biochem 2004;324:29-38.
3. Dionex Corporation. Installation instructions and troubleshooting guide for the AAA-Direct Amino Acid Analysis System. Document No. 031481, Version 8; August 11, 2003.
4. Paul J. Cell and Tissue Culture, 5th ed. New York: Churchill Livingstone; 1975.