Drop-in, drop-out allele-specific PCR: A highly sensitive, single-tube method

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 28, Issue 3, 2004, Pages 171-174

  Alexander, Alice ^a   Subramanian, Nivedita ^a   Buxbaum, Joel N ^b   Jacobson, Daniel R ^a,c  

^a DEPARTMENT OF VETERANS AFFAIRS   (United States)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

^c BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Allele frequency; Allele specific PCR; Drop in, drop out PCR; Mutation frequency; Nested PCR; Transthyretin

Indexed keywords

CODON; POLYMERASE CHAIN REACTION (PCR); RISK OF CONTAMINATION; VARIABLE CONCENTRATION;

BIOASSAY; CONCENTRATION (PROCESS); DNA; ENZYME KINETICS; PROBLEM SOLVING; SENSITIVITY ANALYSIS;

MOLECULAR BIOLOGY;

AGAROSE; ETHIDIUM BROMIDE; PRIMER DNA;

ALLELE; AMYLOIDOSIS; ARTICLE; DNA DETERMINATION; GENE IDENTIFICATION; GENE MUTATION; GENETIC VARIABILITY; GENOTYPE; HIGH THROUGHPUT SCREENING; HUMAN; POLYMERASE CHAIN REACTION; POPULATION GENETICS; AFRICAN AMERICAN; METHODOLOGY; NUCLEOTIDE SEQUENCE;

AFRICAN AMERICANS; ALLELES; BASE SEQUENCE; DNA PRIMERS; HUMANS; POLYMERASE CHAIN REACTION;

EID: 21644469889     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:28:3:171     Document Type: Article

References (7)

1. Sarkar, G., Cassady, J., Bottema, C., and Sommer, S. (1990) Characterization of polymerase chain reaction amplification of specific alleles. Anal. Biochem. 186, 64-68.
2. Erlich, H. A., Gelfand, D., and Sninsky, J.J. (1991) Recent advances in the polymerase chain reaction. Science 252, 1643-1651.
3. Pecharatana, S., Pickett, M. A., Watt, P. J., and Ward, M. E. (1993) Genotyping ocular strains of Chlamydia trachomatis by single-tube nested PCR. PCR Methods Appl. 3, 200-204.
4. Tilston, P. and Corbitt, G. (1995) A single tube nested PCR for the detection of hepatitis C virus RNA. J. Virol. Methods 53, 121-129.
5. Jacobson, D. R. (1992) A specific test for transthyretin 122 (Val-Ile) based on PCR-primer introduced restriction analysis (PCR-PIRA): confirmation the gene frequency in Blacks. Am. J. Hum. Genet. 50, 195-198.
6. Jacobson, D. R., Pastore, R., Pool, S., et al. (1996) Revised transthyretin Ile 122 allele prevalence in African-Americans. Hum. Genet. 98, 236-238.
7. Jacobson, D. R., Pastore, R. D., Yaghoubian, R., et al. (1997) Variant-sequence transthyretin (isoleucine 122) in late-onset cardiac amyloidosis in black Americans. N. Engl. J. Med. 336, 466-473.