A fragment of the hypophosphatemic factor, MEPE, requires inducible cyclooxygenase-2 to exert potent anabolic effects on normal human marrow osteoblast precursors

Journal of Cellular Biochemistry

Volumn 93, Issue 6, 2004, Pages 1107-1114

  Nagel, D E ^a   Khosla, S ^a   Sanyal, A ^a   Rosen, D M ^b   Kumagai, Y ^b   Riggs, B Lawrence ^a  

^a MAYO CLINIC   (United States)

^b Acologix Inc   (United States)

Author keywords

Anabolic agent; BMP 2; Bone formation; COX 2

Indexed keywords

ALKALINE PHOSPHATASE; AMINO ACID; BONE MORPHOGENETIC PROTEIN 2; CYCLOOXYGENASE 2; CYCLOOXYGENASE 2 INHIBITOR; MESSENGER RNA; N (2 CYCLOHEXYLOXY 4 NITROPHENYL)METHANESULFONAMIDE; OSTEOCALCIN; PROCOLLAGEN; PROTEIN AC 100; PROTEIN MEPE; SCLEROPROTEIN; SOMATOMEDIN C; THYMIDINE; UNCLASSIFIED DRUG; BIOLOGICAL MARKER; BMP2 PROTEIN, HUMAN; BONE MORPHOGENETIC PROTEIN; GLYCOPROTEIN; MEMBRANE PROTEIN; MEPE PROTEIN, HUMAN; PHOSPHOPROTEIN; PROSTAGLANDIN SYNTHASE; PTGS2 PROTEIN, HUMAN; TRANSFORMING GROWTH FACTOR BETA;

ALPHA CHAIN; ARTICLE; BONE MARROW; CELL COUNT; CELL DIFFERENTIATION; CELL MATURATION; CELL PROLIFERATION; DRUG MECHANISM; GENE EXPRESSION; HUMAN; HUMAN CELL; HYPOPHOSPHATEMIA; IN VITRO STUDY; MICROARRAY ANALYSIS; OSTEOBLAST; PRIORITY JOURNAL; PROSTAGLANDIN SYNTHESIS; PROTEIN FOLDING; REAL TIME POLYMERASE CHAIN REACTION; STROMA CELL; DNA MICROARRAY; ENZYME ACTIVATION; GENE EXPRESSION PROFILING; MESODERM; METABOLISM;

BIOLOGICAL MARKERS; BONE MARROW; BONE MORPHOGENETIC PROTEINS; CELL DIFFERENTIATION; CELL PROLIFERATION; CYCLOOXYGENASE 2; ENZYME ACTIVATION; EXTRACELLULAR MATRIX PROTEINS; GENE EXPRESSION PROFILING; GLYCOPROTEINS; HUMANS; INSULIN-LIKE GROWTH FACTOR I; MEMBRANE PROTEINS; MESODERM; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; OSTEOBLASTS; PHOSPHOPROTEINS; PROSTAGLANDIN-ENDOPEROXIDE SYNTHASES; STROMAL CELLS; TRANSFORMING GROWTH FACTOR BETA;

EID: 21644453949     PISSN: 07302312     EISSN: 10974644     Source Type: Journal    
DOI: 10.1002/jcb.20249     Document Type: Article

References (26)

1. Argiro L, Desbarats M, Glorieux FH, Ecarot B. 2001. MEPE, the gene encoding a tumor-secreted protein in oncogenic hypophosphatemic osteomalacia, is expressed in bone. Genomics 74:342-351.
2. Aubin JE, Triffitt JT. 2002. Mesenchymal stem cells and osteoblast differentiation. In: Bilezikian JP, Raisz LG, Rodan GA, editors. Principles of bone biology, 2nd edn. New York: Academic Press, pp 59-81.
3. Bakker AD, Klein-Nulend J, Burger EH. 2003. Mechotransduction in bone cells prodeeds via activation of COX-2, but not COX-1. Biochem Biophys Res Commun 305:677-683.
4. Canalis E, Economides AN, Gazzerro E. 2003. Bone morphogenetic proteins, their antagonists, and the skeleton. Endocr Rev 24:218-235.
5. Ducy P, Zhang R, Geoffroy V, Ridall AL, Karsenty G. 1997. Osf2/Cbfa1: A transcriptional activator of osteoblast differentiation. Cell 89:747-754.
6. 2. Endocrinology 130:443-448.
7. Forwood MR. 1996. Inducible cyclo-oxygenase (COX-2) mediates the induction of bone formation by mechanical loading in vivo. J Bone Miner Res 11:1688-1693.
8. Gerstenfeld LC, Thiede M, Mielke C, Phippard D, Svangr B, Cullinane D, Einhorn TA. 2003. Differential inhibition of fracture healing by non-selective and cyclooxygenase-2 selcetive non-steroidal anti-inflamatory drugs. J Orthop Res 21:670-675.
9. Gori F, Thomas T, Hicok KC, Spelsberg TC, Riggs BL. 1999. Differentiation of human marrow stromal precursor cells: Bone morphogenetic protein-2 increases OSF2/CBFA1, enhances osteoblast commitment, and inhibits late adipocyte maturation. J Bone Miner Res 14:1522-1535.
10. Gowen LC, Petersen DN, Mansolf AL, Qi H, Stock JL, Tkalcevic GT, Simmons HA, Crawford DT, Chidsey-Frink KL, Ke HZ, McNeish JD, Brown TA. 2003. Targeted disruption of the osteoblast/osteocyte factor 45 gene (OF45) results in increased bone formation and bone mass. J Biol Chem 278:1998-2007.
11. Hayashibara T, Hiraga T, Yi B, Nomizu M, Kumagai Y, Nishimura R, Yoneda T. 2004. A synthetic peptide fragment of human MEPE stimulates new bone formation in vitro and in vivo. J Bone Miner Res 19:455-462.
12. Jee WS, Ma YF. 1997. The in vivo anabolic actions of prostaglandins in bone. Bone 21:287-304.
13. Jones JI, Clemmons DR. 1995. Insulin-like growth factors and their binding proteins: Biological actions. Endocr Rev 16:3-34.
14. Locklin RM, Riggs BL, Hicok KC, Horton HF, Byrne MC, Khosla S. 2001. Assessment of gene regulation by bone morphogenetic protein 2 in human marrow stromal cells using gene array technology. J Bone Miner Res 16:2192-2204.
15. Nakashima K, Zhou X, Kunkel G, Zhang Z, Deng JM, Behringer RR, Crombrugghe BD. 2002. The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation. Cell 108:17-29.
16. Petersen DN, Tkalcevic GT, Mansolf AL, Rivera-Gonzalez R, Brown TA. 2000. Identification of osteoblast/osteocyte factor 45 (OF45), a bone-specific cDNA encoding an RGD-containing protein that is highly expressed in osteoblasts and osteocytes. J Biol Chem 275:36172-36180.
17. Pilbeam CC, Harrison JR, Raisz LG. 2002. Chapter 54. Prostaglandins and bone metabolism. In: Bilezikian JP, Raisz LG, Rodan GA, editors. Principles of bone biology, 2nd edn. New York: Academic Press, pp 979-994.
18. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR. 1999. Multilineage potential of adult human mesenchymal stem cells. Science 284:143-147.
19. Ro J, Sudmann E, Marion PF. 1976. Effect of indomethacin on fracture healing in rats. Acta Orthop Scand 47:588-599.
20. Rowe PSN, Ong ACM, Cockerill FJ, Goulding JN, Hewison M. 1996. Candidate 56 and 58 kDa protein(s) responsible for mediating the renal defects in oncogenic hypophosphatemic osteomalacia. Bone 18:159-169.
21. Rowe PSN, de Zoysa PA, Dong R, Wang HR, White KE, Econs MJ, Oudet CL. 2000. MEPE, a new gene expressed in bone marrow and tumors causing osteomalacia. Genomics 67:54-68.
22. Rowe PSN, Kumagai Y, Gutierrez G, Garrett IR, Blacher R, Rosen D, Cundy J, Navvab S, Chen D, Drezner MK, Querles LD, Mundy GR. 2004. MEPE has the properties of an osteoblastic phosphatonin and minhibin. Bone 34:303-319.
23. Simon AM, Manigrasso MB, O'Connor JP. 2002. Cyclooxygenase 2 function is essential for bone fracture healing. J Bone Miner Res 17:963-976.
24. Stein GS, Lian JB, Owen TA. 1990. Bone cell differentiation: A functionally coupled relationship between expression of cell-growth and tissue-specific genes. Curr Opin Cell Biol 2:1018-1027.
25. 2 on bone mass in rat long bones and calvariae. J Endocrinol 156:51-57.
26. Thomas T, Gori F, Spelsberg TC, Khosla S, Riggs BL, Conover C. 1999. Response of bipotential human marrow stromal cells to insulin-like growth factors: Effect on binding protein production, proliferation, and commitment to osteoblasts and adipocytes. Endocrinology 140:5036-5044.