On-column refolding of an insoluble His6-tagged recombinant EC-SOD overexpressed in Escherichia coli

Acta Biochimica et Biophysica Sinica

Volumn 37, Issue 4, 2005, Pages 265-269

  Zhu, Xi Qiang ^a   Li, Su Xia ^a   He, Hua Jun ^a   Yuan, Qin Sheng ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

His6 tag; Inclusion body; Protein subunit structure; Recombinant EC SOD; Refolding

Indexed keywords

COMPLEMENTARY DNA; DIMER; DISULFIDE; GLUTATHIONE; GLUTATHIONE DISULFIDE; HISTIDINE; MONOMER; PROTEIN SUBUNIT; RECOMBINANT ENZYME; RECOMBINANT EXTRACELLULAR SUPEROXIDE DISMUTASE; SUPEROXIDE DISMUTASE; UNCLASSIFIED DRUG; UREA;

ARTICLE; BACTERIUM TRANSFORMATION; CELL INCLUSION; DISULFIDE BOND; DRUG SYNTHESIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME DENATURATION; ENZYME ELECTROPHORESIS; ENZYME ENGINEERING; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SUBUNIT; ENZYME SYNTHESIS; ESCHERICHIA COLI; EXTRACELLULAR SPACE; GENE INSERTION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MASS; MOLECULAR CLONING; MOLECULAR WEIGHT; PLASMID VECTOR; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN FOLDING; SOLUBILIZATION; ULTRAFILTRATION;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; HISTIDINE; HUMANS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; RECOMBINANT PROTEINS; SOLUBILITY; SUPEROXIDE DISMUTASE;

ESCHERICHIA COLI;

EID: 21644449572     PISSN: 16729145     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1745-7270.2005.00035.x     Document Type: Article

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