The disruption of Saccharomyces cerevisiae cells and release of glucose 6-phosphate dehydrogenase (G6PDH) in a horizontal dyno bead mill operated in continuous recycling mode

Biotechnology and Bioprocess Engineering

Volumn 10, Issue 3, 2005, Pages 284-288

  Mei, Chow Yen ^a   Ti, Tey Beng ^a   Ibrahim, Mohammad Nordin ^a   Ariff, Arbakariya ^a   Chuan, Ling Tau ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

Author keywords

Cell disruption; Dyno bead mill; Glucose 6 phosphate dehydrogenase; Yeast cells; Zirconia beads

Indexed keywords

GLUCOSE 6 PHOSPHATE DEHYDROGENASE; ZIRCONIUM OXIDE;

ARTICLE; BIOMASS; CELL DISRUPTION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME RELEASE; FLOW RATE; HEAT; NONHUMAN; PROTEIN SECRETION; SACCHAROMYCES CEREVISIAE; VISCOSITY; WASTE WATER RECYCLING; YEAST CELL;

SACCHAROMYCES CEREVISIAE;

EID: 21644433415     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02932027     Document Type: Article

References (12)

1. Middlelberg, A. P. J. (1995) Process scale disruption of microorganisms. Biotechnol. Adv. 13: 491-550.
2. Limon-Lason, J., J. Hoare, C. B. Orsborn, D. J. Doyle, and P. Dunnill (1979) Reactor properties of a high-speed bead mill for microbial cell rupture. Biotechnol. Bioeng. 21: 745-774.
3. Schutte, H., K. H. Kroner, H. Hustedt, and M-R. Kula (1983) Experiences with a 20 litre industrial bead mill for the disruption of microorganisms. Enzyme Microb. Technol. 5: 143-148.
4. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem. 72: 248-254.
5. Bergmeyer, H. U., M. Grabl, and H. E. Walter (1983) Biochemical reagents for general use: Enzymes. pp. 222-223. In: Bergmeyer, U. (ed.). Methods in Enzymatic Analysis. Academic Press, NY, USA.
6. Kula, M.-R. and H. Schütte (1987) Purification of proteins and the disruption of microbial cells. Biotechnol. Prog. 3: 31-42.
7. Ricci-Silva, M. E., M. Vitolo, and J. Abrahão-Neto (2000) Protein and glucose 6-phosphate dehydrogenase releasing from baker's yeast cells disrupted by a vertical bead mill. Process Biochemistry 35: 831-835.
8. Garrido, F., U. C. Banerjee, Y. Chisti, and M. Moo-Young (1994) Disruption of a recombinant yeast for the release of β-galactosidase. Bioseparation 4: 319-328.
9. Garcia, F. A. P. (1993) Cell wall disruption. pp. 47-67. In: Kennedy, J. F. and J. M. S. Cabral (eds.). Recovery Processes for Biological Materials. John Wiley and Sons, Chichester, UK.
10. Melendres, A. V., H. Honda, N. Shiragami, and H. Unno (1991) A kinetic analysis of cell disruption by bead mill. Bioseparation 2: 231-236.
11. Melendres, A. V., H. Unno, N. Shiragami, and H. Honda (1992) A concept of critical velocity for cell disruption by bead mill. J. Chemical Eng. Japan 25: 354-356.
12. Mario Canales, J. A., L. H. Buxado, and E. Antonio (1998) Mechanical disruption of Pichia pastoris yeast to recover the recombinant glycoprotein Bm86. In: Genetic Engineering and Biotechnol. Havana, Cuba.