Extent of aminoglycoside interference in the pyrogallol red-molybdate protein assay depends on the concentration of sodium oxalate in the dye reagent

Clinical Chemistry

Volumn 50, Issue 5, 2004, Pages 934-935

  Marshall, Thomas ^a   Williams, Katherine M ^a  

^a UNIVERSITY OF SUNDERLAND   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMINOGLYCOSIDE; MOLYBDIC ACID; OXALATE SODIUM; OXALIC ACID DERIVATIVE; PYROGALLOL; REAGENT; UNCLASSIFIED DRUG;

ARTICLE; CONCENTRATION RESPONSE; PROTEIN DETERMINATION; URINALYSIS;

AMINOGLYCOSIDES; COLORING AGENTS; FALSE POSITIVE REACTIONS; HUMANS; MOLYBDENUM; OXALATES; PROTEINURIA; PYROGALLOL;

EID: 2142826520     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2003.030478     Document Type: Article

References (8)

1. Fujita Y, Mori I, Kitano S. Color reaction between pyrogallol red-molybdenum(VI) complex and protein. Bunseki Kagaku 1983;32:E379-86.
2. Watanabe N, Kamei S, Ohkubo A, Yamanaka M, Ohsawa S, Makino K, et al. Urinary protein as measured with a pyrogallol red molybdate complex manually and in a Hitachi 726 automated analyzer. Clin Chem 1986;32: 1551-4.
3. Koerbin G, Taylor L, Dutton J, Marshall K, Low P, Potter JM. Aminoglycoside interference with the Dade Behring pyrogallol red-molybdate method for the measurement of total urine protein [Letter]. Clin Chem 2001;47:2183-4.
4. Marshall T, Williams KM. Total protein determination in urine: aminoglycoside interference [Letter]. Clin Chem 2003;49:202-3.
5. Parfitt K, ed. Martindale the complete drug reference, 32nd ed. Taunton: Pharmaceutical Press, 1999:112-270.
6. Dollery C, ed. Therapeutic drugs, Vol. 1. London: Churchill Livingstone, 1991: A1-I131.
7. Orsonneau JL, Douet P, Massoubre C, Lustenberger P, Bernard S. An improved pyrogallol red-molybdate method for determining total urinary protein. Clin Chem 1989;35:2233-5.
8. Marshall T, Williams KM. Aminoglycoside interference in the pyrogallol red-molybdate protein assay is increased by the addition of sodium dodecyl sulfate to the dye reagent [Letter]. Clin Chem 2003;49:2111-2.