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S.GOD, a value of 120 mM at pH 5.6, 38°C, was reported in: Gibson, Q. H.; Swoboda, B. E. P.; Massay, V. J. Biol. Chem. 1964, 239, 3927.
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85085782925
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note
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2 at concentrations higher than 1 M, an absorption band around 415 nm increased faster than that of 520 nm. Therefore, the former band can be assigned to BQI. This is also supported by considering the longest π-π* wavelength of p-benzoquinone (425 nm). The shoulder around 430 nm in Figure 1A would be identical with the 415 nm band by considering the overlapping of the 520 nm band.
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14
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0022287870
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For the Cottrell equation, see, for example: Bard A. J.; Faulkner L. R. In Electrochemical Methods; Wiley: New York, 1980; 1980; p 142. In our case, chronoamperometric measurements were done with a potential step from 0 to 0.35 V. The resulting i√t values were practically constant at least within a time (t) range from 1 to 12 s.
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3643146183
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note
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M(BQI) value seems to be smaller than that estimated here, because the latter involves the depletion effect of glucose near the electrode surface.
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32
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0000661330
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38
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3643118045
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note
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Under storage at 40°C, over 90% of the UOD activity was retained after 6 months.
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