Northerns revisited: A protocol that eliminates formaldehyde from the gel while enhancing resolution and sensitivity

Analytical Biochemistry

Volumn 342, Issue 2, 2005, Pages 356-357

  Vincze, Eva ^a   Bowra, Steve ^a  

^a DANISH INSTITUTE OF AGRICULTURAL SCIENCES   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

CHEMISTRY;

BROMOPHENOL BLUE; EDETIC ACID; ETHIDIUM BROMIDE; FORMALDEHYDE; FORMAMIDE; XYLENE;

ARTICLE; CHEMICAL REACTION KINETICS; GEL; HEATING; NORTHERN BLOTTING; PH MEASUREMENT; PRIORITY JOURNAL;

EID: 21244501206     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2005.02.026     Document Type: Article

References (8)

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2. G.G. Carmichael, and G.K. McMaster The analysis of nucleic acids in gels using glyoxal and acridine orange Methods Enzymol. 63 1980 380 391
3. H.D. Lehrach, D. Diamond, J.M. Wozney, and H. Boedtker RNA molecular weight determinations by gel electrophoresis under denaturing conditions: a critical reexamination Biochemistry 16 1977 4743 4751
4. T. Maniatis, E.F. Fritsch, and J. Sambrook Molecular Cloning: A Laboratory Manual 1989 Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY
5. G.F. Gerard, and K. Miller Comparison of glyoxal and formaldehyde gels for sizing rRNAs Focus 19 1997 17 19
6. B. Ogretmen, H. Ratajczak, A. Kats, and B.C. Stark Effects of staining of RNA with EtBr before electrophoresis on performance of Northern blots BioTechniques 14 1993 932 935
7. L. Davis, M. Kuehl, and J. Battey Formaldehyde agarose gel electrophoresis and RNA (Northern) blots second ed. Basic Methods in Molecular Biology 1994 Appleton & Lange Norwalk, CT 350 354
8. P. Chomczynski One-hour downward alkaline capillary transfer for blotting of DNA and RNA Anal. Biochem. 201 1992 134 139