메뉴 건너뛰기




Volumn 22, Issue 3, 2005, Pages 323-330

Purification of recombinant aprotinin produced in transgenic corn seed: Separation from cti utilizing ion-exchange chromatography

Author keywords

Downstream processing; Ion exchange chromatography; Protease inhibitor purification; Recombinant aprotinin; Transgenic corn

Indexed keywords

ADSORPTION ISOTHERMS; CHROMATOGRAPHIC ANALYSIS; ENZYME INHIBITION; ION EXCHANGE; PH EFFECTS; PLANTS (BOTANY); PURIFICATION; SEED; SEPARATION;

EID: 21244497353     PISSN: 01046632     EISSN: None     Source Type: Journal    
DOI: 10.1590/S0104-66322005000300001     Document Type: Article
Times cited : (12)

References (18)
  • 1
    • 0023425920 scopus 로고
    • Synthesis, cloning and expression of recombinant aprotinin
    • Auerswald, E.A., Schröder, W. and Kotick, M., Synthesis, cloning and expression of recombinant aprotinin, Biol. Chem. Hoppe-Seyler, 368, 1413-1425 (1987).
    • (1987) Biol. Chem. Hoppe-Seyler , vol.368 , pp. 1413-1425
    • Auerswald, E.A.1    Schröder, W.2    Kotick, M.3
  • 2
    • 0037027397 scopus 로고    scopus 로고
    • Recombinant aprotinin produced in transgenic corn seed: Extraction and purification studies
    • Azzoni, A.R., Kusnadi, A.R., Miranda, E.A. and Nikolov, Z.L., Recombinant aprotinin produced in transgenic corn seed: extraction and purification studies, Biotechnol. Bioeng., 80, 268-276 (2002).
    • (2002) Biotechnol. Bioeng. , vol.80 , pp. 268-276
    • Azzoni, A.R.1    Kusnadi, A.R.2    Miranda, E.A.3    Nikolov, Z.L.4
  • 3
    • 0032779099 scopus 로고    scopus 로고
    • Recovery of aprotinin from insulin industrial process effluent by affinity adsorption
    • Azzoni, A.R. and Miranda, E.A., Recovery of aprotinin from insulin industrial process effluent by affinity adsorption, Bioproc. Eng., 21, 553-560, (1999).
    • (1999) Bioproc. Eng. , vol.21 , pp. 553-560
    • Azzoni, A.R.1    Miranda, E.A.2
  • 4
    • 0033861084 scopus 로고    scopus 로고
    • Plasma aprotinin concentrations during cardiac surgery: Full-dose versus half-dose regimens
    • Beath, S.M., Nuttall, G.A., Fass, D.N., Oliver Jr, W.C., Ereth, M.H. and Oyen, L.J., Plasma aprotinin concentrations during cardiac surgery: full-dose versus half-dose regimens, Anesth. Analg., 91, 257-264 (2000).
    • (2000) Anesth. Analg. , vol.91 , pp. 257-264
    • Beath, S.M.1    Nuttall, G.A.2    Fass, D.N.3    Oliver Jr., W.C.4    Ereth, M.H.5    Oyen, L.J.6
  • 5
    • 0003136996 scopus 로고    scopus 로고
    • Transgenic plants for therapeutic proteins: Linking upstream and downstream strategies
    • Hammond, J., McGarvey, P. and Yusibov, V., Eds., New York, Springer
    • Cramer, C.L., Boothe, J.G. and Oishi, K.K., Transgenic plants for therapeutic proteins: linking upstream and downstream strategies. In "Plant biotechnology: new products and applications", Hammond, J., McGarvey, P. and Yusibov, V., Eds., New York, Springer, pp. 95-115 (2000).
    • (2000) Plant Biotechnology: New Products and Applications , pp. 95-115
    • Cramer, C.L.1    Boothe, J.G.2    Oishi, K.K.3
  • 6
    • 0019129293 scopus 로고
    • Hageman factor fragment inhibitor in corn seeds: Purification and characterization
    • Hojima, Y., Pierce, J.V. and Pisano, J.J., Hageman factor fragment inhibitor in corn seeds: purification and characterization, Thromb. Research, 20, 149-162 (1980).
    • (1980) Thromb. Research , vol.20 , pp. 149-162
    • Hojima, Y.1    Pierce, J.V.2    Pisano, J.J.3
  • 7
    • 1642576693 scopus 로고    scopus 로고
    • Protein products from transgenic plants
    • Hood, E.E. and Howard, J.A., Protein products from transgenic plants, Agro-Food-Industry Hi-Tech., May/June, pp 35-36 (1999).
    • (1999) Agro-Food-Industry Hi-Tech. , Issue.MAY-JUNE , pp. 35-36
    • Hood, E.E.1    Howard, J.A.2
  • 8
    • 77956984806 scopus 로고
    • Bovine trypsin-kallikrein inhibitor (Kunitz inhibitor, basic pancreatic trypsin inhibitor, polyvalent inhibitor from bovine organs)
    • Kassel, B., Bovine trypsin-kallikrein inhibitor (Kunitz inhibitor, basic pancreatic trypsin inhibitor, polyvalent inhibitor from bovine organs), Meth. Enzymol., 19, 844-852 (1970).
    • (1970) Meth. Enzymol. , vol.19 , pp. 844-852
    • Kassel, B.1
  • 9
    • 85025382997 scopus 로고
    • Isolation from beef pancreas of crystalline trypsinogen, trypsin inhibitor, and an inhibitor trypsin compound
    • Kunitz, M. and Northrop, J.H., Isolation from beef pancreas of crystalline trypsinogen, trypsin inhibitor, and an inhibitor trypsin compound, J. Gen. Physiol., 19, 991-1007 (1936).
    • (1936) J. Gen. Physiol. , vol.19 , pp. 991-1007
    • Kunitz, M.1    Northrop, J.H.2
  • 10
    • 0031555505 scopus 로고    scopus 로고
    • Production of recombinant proteins in transgenic plants: Practical considerations
    • Kusnadi, A.R., Nikolov, Z.L. and Howard, J.A., Production of recombinant proteins in transgenic plants: practical considerations, Biotechnol. Bioeng., 56, 473-484 (1997).
    • (1997) Biotechnol. Bioeng. , vol.56 , pp. 473-484
    • Kusnadi, A.R.1    Nikolov, Z.L.2    Howard, J.A.3
  • 11
    • 4043158815 scopus 로고
    • The constitution and fundamental properties of solids and liquids
    • Langmuir, I., The constitution and fundamental properties of solids and liquids, J. Amer. Chem. Soc., 38, 2263-2295 (1916).
    • (1916) J. Amer. Chem. Soc. , vol.38 , pp. 2263-2295
    • Langmuir, I.1
  • 12
    • 0023058938 scopus 로고
    • Combined use of trypsin-agarose affinity chromatography and reversed-phase high-performance liquid chromatography for the purification of single-chain protease inhibitor from corn seeds
    • Lei, M.G. and Reeck, G.R., Combined use of trypsin-agarose affinity chromatography and reversed-phase high-performance liquid chromatography for the purification of single-chain protease inhibitor from corn seeds, J. Chromat., 363, 315-321 (1986).
    • (1986) J. Chromat. , vol.363 , pp. 315-321
    • Lei, M.G.1    Reeck, G.R.2
  • 14
    • 21244491821 scopus 로고    scopus 로고
    • on April 9
    • Prodigene. Internet site www.prodigene.com/pdf/AproliZean (tm)%20Backgrouder.pdf, on April 9, 2004.
    • (2004)
  • 15
    • 0017691198 scopus 로고
    • Isolation and characterization of trypsin inhibitor from opaque-2 corn seeds
    • Swartz, M.J., Mitchell, H.L., Cox, D.J. and Reeck, G.R., Isolation and characterization of trypsin inhibitor from opaque-2 corn seeds, J. Biol. Chem., 252, No. 22, 8105-8107 (1977).
    • (1977) J. Biol. Chem. , vol.252 , Issue.22 , pp. 8105-8107
    • Swartz, M.J.1    Mitchell, H.L.2    Cox, D.J.3    Reeck, G.R.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.