High-throughput chemiluminometric determination of prostate-specific membrane antigen mRNA in peripheral blood by RT-PCR using a synthetic RNA internal standard

Analytical and Bioanalytical Chemistry

Volumn 380, Issue 1, 2004, Pages 90-97

  Emmanouilidou, Evaggelia ^a   Ioannou, Penelope C ^a   Christopoulos, Theodore K ^b,c  

^a UNIVERSITY OF ATHENS   (Greece)

^b UNIVERSITY OF PATRAS   (Greece)

^c INSTITUTE OF CHEMICAL ENGINEERING SCIENCES   (Greece)

Author keywords

Chemiluminescence; Hybridization; Peripheral blood; PSMA mRNA; Quantitative RT PCR

Indexed keywords

ANTIGENS; BIOASSAY; BLOOD; CELL MEMBRANES; CELLS; CHEMILUMINESCENCE; ENZYMES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; OLIGOMERS; PURIFICATION; TUMORS;

HYBRIDIZATION; PERIPHERAL BLOOD; PROSTRATE-SPECIFIC MEMBRANE ANTIGEN (PSMA) MRNA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION (RT-PCR);

RNA;

ALKALINE PHOSPHATASE; DNA; MEMBRANE ANTIGEN; MESSENGER RNA; OLIGONUCLEOTIDE; RECOMBINANT PROTEIN; RNA;

ARTICLE; BLOOD; CALIBRATION; CHEMICAL STRUCTURE; CHEMISTRY; CHEMOLUMINESCENCE; FEMALE; GENETICS; HUMAN; METHODOLOGY; REFERENCE VALUE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STANDARD; SYNTHESIS; TUMOR CELL LINE;

ALKALINE PHOSPHATASE; ANTIGENS, SURFACE; CALIBRATION; CELL LINE, TUMOR; CHEMILUMINESCENT MEASUREMENTS; DNA; FEMALE; HUMANS; MOLECULAR STRUCTURE; OLIGONUCLEOTIDES; RECOMBINANT PROTEINS; REFERENCE STANDARDS; REFERENCE VALUES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY;

EID: 21044438625     PISSN: 16182642     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00216-004-2719-6     Document Type: Article

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