Enzymatic synthesis of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) with CoA recycling using polyhydroxyalkanoate synthase and acyl-CoA synthetase

Journal of Bioscience and Bioengineering

Volumn 99, Issue 5, 2005, Pages 508-511

  Satoh, Yasuharu ^a   Murakami, Fumikazu ^a   Tajima, Kenji ^a   Munekata, Masanobu ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

Author keywords

Acyl CoA synthetase; Enzymatic synthesis; Kanamycin; Poly(3 hydroxybutyrate co 4 hydroxybutyrate); Polyhydroxyalkanoate

Indexed keywords

ENZYMES; MIXTURES; MONOMERS; RECYCLING; SYNTHESIS (CHEMICAL);

ENZYMATIC SYNTHESIS; POLYHYDROXYALKANOATE SYNTHASE; REACTION MIXTURES;

ORGANIC POLYMERS;

COENZYME A; KANAMYCIN; LONG CHAIN FATTY ACID COENZYME A LIGASE; MONOMER; POLY(3 HYDROXYBUTYRATE CO 4 HYDROXYBUTYRATE); POLY(3 HYDROXYBUTYRIC ACID); POLYHYDROXYALKANOATE SYNTHASE; POLYHYDROXYALKANOIC ACID; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL COMPOSITION; ENZYME MECHANISM; ESCHERICHIA COLI; IN VITRO STUDY; NONHUMAN; PLASMID; POLYMERIZATION; RECYCLING; SYNTHESIS;

EID: 21044433212     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.99.508     Document Type: Article

References (23)

1. Rehm, B. H. and Steinbüchel, A.: Biochemical and genetic analysis of PHA synthases and other proteins required for PHA synthesis. Int. J. Biol. Macromol., 25, 3-19 (1999).
2. Lee, S. Y., Choi, J., and Wong, H. H.: Recent advances in polyhydroxyalkanoate production by bacterial fermentation: mini-review. Int. J. Biol. Macromol., 25, 31-36 (1999).
3. Jendrossek, D., Schirmer, A., and Schlegel, H. G.: Biodegradation of polyhydroxyalkanoic acids. Appl. Microbiol. Biotechnol., 46, 451-463 (1996).
4. Jendrossek, D. and Handrick, R.: Microbiol degradation of polyhydroxyalkanoates. Annu. Rev. Microbiol., 56, 403-432 (2002).
5. Gerngross, T. U. and Martin, D. P.: Enzyme-catalyzed synthesis of poly[(R)-(-)-3-hydroxybutyrate]: formation of macroscopic granules in vitro. Proc. Natl. Acad. Sci. USA, 92, 6279-6283 (1995).
6. Gerngross, T. U., Snell, K. D., Peoples, O. P., Synsky, A. J., Csuhai, E., Masamune, S., and Stubbe, J.: Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity. Biochemistry, 33, 9311-9320 (1994).
7. Jossek, R., Reichelt, R., and Steinbüchel, A.: In vitro biosynthesis of poly(3-hydroxybutyric acid) by using purified poly(hydroxyalkanoic acid) synthase of Chromatium vinosum. Appl. Microbiol. Biotechnol., 49, 258-266 (1998).
8. Liebergesell, M., Sonomoto, K., Madkour, M., Mayer, F., and Steinbüchel, A.: Purification and characterization of the poly(hydroxyalkanoic acid) synthase from Chromatium vinosum and localization of the enzyme at the surface of polyhydroxyalkanoic acid) granules. Eur. J. Biochem., 226, 71-80 (1994).
9. Satoh, Y., Minamoto, N., Tajima, K., and Munekata, M.: Polyhydroxybutyrate synthase from Bacillus sp. INT005 is composed of PhaC and PhaR. J. Biosci. Bioeng., 94, 343-350 (2002).
10. Song, J. J., Zhang, S., Lenz, R. W., and Goodwill, S.: In vitro polymerization and copolymerization of 3-hydroxypropionyl-CoA with the PHB synthase from Ralstonia eutropha. Biomacromolecules, 1, 433-439 (2000).
11. Su, L., Lenz, R. W., Takagi, Y., Zhang, S., Goodwill, S., Zhong, L., and Martin, D. P.: Enzymatic polymerization of (R)-3-hydroxyalkanoates by a bacterial polymerase. Macromolecules, 33, 229-231 (2000).
12. Williams, M. D., Fieno, A. M., Grant, R. A., and Sherman, D. H.: Expression and analysis of a bacterial poly(hydroxyalkanoate) synthase in insect cells using a baculovirus system. Protein Expr. Purif., 7, 203-211 (1996).
13. Yuan, W., Jia, Y., Tian, J., Snell, K. D., Muh, U., Sinskey, J., Lambalt, R. H., Walsh, C. T., and Stubbe, J.: Class I and III polyhydroxyalkanoate synthase from Ralstonia eutropha and Allochromatium vinosum: characterization and substrate specificity studies. Arch. Biochem. Biophys., 394, 87-98 (2001).
14. Zhang, S., Kamachi, M., Takagi, Y., Lenz, R. W., and Goodwin, S.: Comparative study of the relationship between monomer structure and reactivity for two polyhydroxyalkanoate synthases. Appl. Microbiol. Biotechnol., 56, 131-136 (2001).
15. Zhang, S., Takagi, Y., Lenz, R. W., and Goodwin, S.: Kinetic and mechanistic characterization of the polyhydroxybutyrate synthase from Ralstonia eutropha. Biomacromolecules, 1, 244-251 (2000).
16. Jossek, R. and Steinbüchel, A.: In vitro synthesis of poly(3-hydroxybutyric acid) by using an enzymatic coenzyme A recycling system. FEMS Microbiol. Lett., 168, 319-324 (1998).
17. Liu, S.-J. and Steinbüchel, A.: Exploitation of butyrate kinase and phosphotransbutyrylase from Clostridium acetobutylicum for the in vitro biosynthesis of polyfliydroxyalkanoic acid). Appl. Microbiol. Biotechnol., 53, 545-552 (2000).
18. Satoh, Y., Tajima, K., Tannai, H., and Munekata, M.: Enzyme-catalyzed poly(3-hydroxybutyrate) synthesis from acetate with CoA recycling and NADPH regeneration in vitro. J. Biosci. Bioeng., 95, 335-341 (2003).
19. Eggink, G., Lageveen, R. G., Altenburg, B., and Witholt, B.: Controlled and functional expression of the Pseudomonas oleovolans alkane utilizing system in Pseudomonas putida and Escherichia coli. J. Biol. Chem., 262, 17712-17718 (1987).
20. van Beilen, J. B., Eggink, G., Enequist, H., Bos, R., and Witholt, B.: DNA sequence determination and functional characterization of the OCT-plasmid-encoded alkJKL genes of Pseudomonas oleovorans. Mol. Microbiol, 6, 3121-3136 (1992).
21. Sambrook, J., Fritsch, E. F., and Maniatis, T.: Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989).
22. Tajima, K., Igari, T., Nishimura, D., Nakamura, M., Satoh, Y., and Munekata, M.: Isolation and characterization of Bacillus sp. 1NT005 accumulating polyhydroxyalkanoate (PHA) from gas field soil. J. Biosci. Bioeng., 95, 77-81 (2003).
23. Haywood, G. W., Anderson, A. J., and Dawes, E. A.: The importance of PHB-synthase substrate specificity in polyhydroxyalkanoate synthesis by Alcaligenes eutrophus. FEMS Microbiol. Lett., 57, 1-6 (1989).