Production and characterization of a monoclonal antibody specific for NS3 protease and the ATPase region of dengue-2 virus

Hybridoma

Volumn 24, Issue 3, 2005, Pages 160-164

  Garcia Cordero J ^a   Ramirez, H Romero ^a   Vazquez Ochoa, M ^a   Gutierrez Castaneda B ^b   Santos Argumedo, L ^a   Villegas Sepulveda N ^a   Cedillo Barrón, Leticia ^a  

^a DEPARTAMENTO DE FÍSICA   (Mexico)

^b INSTITUTO POLITÉCNICO NACIONAL   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; MONOCLONAL ANTIBODY; NONSTRUCTURAL PROTEIN 3; NS3 MONOCLONAL ANTIBODY; PROTEINASE; SYNAPSIN II; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY PRODUCTION; ARTICLE; DENGUE; DENGUE VIRUS; DRUG PURIFICATION; DRUG SCREENING; ENDOTHELIUM CELL; ENZYME ACTIVITY; HYBRIDOMA; IMMUNIZATION; IMMUNOFLUORESCENCE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SPLEEN CELL; VIRUS REPLICATION; WESTERN BLOTTING;

DENGUE VIRUS; DENGUE VIRUS TYPE 2;

EID: 20844446165     PISSN: 15540014     EISSN: None     Source Type: Journal    
DOI: 10.1089/hyb.2005.24.160     Document Type: Article

References (21)

1. Gubler DJ: Epidemic dengue/dengue hemorrhagic fever as a public health, social and economic problem in the 21st century. Trends Microbiol 2002;10:100-103.
2. Guzman MG, and Kouri G: Dengue: an update. Lancet Infect Dis 2002;2:33-42.
3. Halstead SB, and Deen J: The future of dengue vaccines. Lancet 2002;360:1243-1245.
4. Halstead SB: Pathogenesis of dengue: challenges to molecular biology. Science 1988;239:476-481.
5. Chambers TJ, Hahn CS, Galler R, and Rice CM: Flavivirus genome organization, expression, and replication. Annu Rev Microbiol 1990;44:649-688.
6. Wengler G, Czaya G, Farber PM, and Hegemann JH: In vitro synthesis of West Nile virus proteins indicates that the amino-terminal segment of the NS3 protein contains the active centre of the protease which cleaves the viral polyprotein after multiple basic amino acids. J Gen Virol 1991;72:851-858.
7. Li H, Clum S, You S, Ebner KE, and Padmanabhan R: The serine protease and RNA-stimulated nucleoside triphosphatase and RNA helicase functional domains of dengue virus type 2 NS3 converge within a region of 20 amino acids. J Virol 1999;73:3108-3116.
8. Clum S, Ebner KE, and Padmanabhan R: Cotranslational membrane insertion of the serine proteinase precursor NS2B-NS3(Pro) of dengue virus type 2 is required for efficient in vitro processing and is mediated through the hydrophobic regions of NS2B. J Biol Chem 1997;272:30715-30723.
9. Kuhn RJ, Zhang W, Rossmann MG, Pletnev SV, Corver J, Lenches E, Jones CT, Mukhopadhyay S, Chipman PR, Strauss EG, Baker TS, and Strauss JH: Structure of dengue virus: implications for flavivirus organization, maturation, and fusion. Cell 2002;108:717-725.
10. - cytotoxic T-cell clones: multiple patterns of virus cross-reactivity recognized by NS3-specific T-cell clones. J Virol 1991;65:1823-1828.
11. Lobigs M, Arthur CE, Mullbacher A, and Blanden RV: The flavivirus nonstructural protein NS3 is a dominant source of cytotoxic T cell peptide determinants. Virology 1994;202:195-201.
12. + cytotoxic T lymphocyte clones cross-reactive for dengue virus types 2, 3, and 4. Virology 1998;240:169-174.
13. Courageot MP, Catteau A, and Despres P: Mechanisms of dengue virus-induced cell death. Adv Virus Res 2003;60:157-186.
14. Catteau A, Roue G, Yuste VJ, Susin SA, and Despres P: Expression of dengue ApoptoM sequence results in disruption of mitochondrial potential and caspase activation. Biochimie 2003;85:789-793.
15. Duarte dos Santos CN, Frenkiel MP, Courageot MP, Rocha CF, Vazeille-Falcoz MC, Wien MW, Rey FA, Deubel V, and Despres P: Determinants in the envelope E protein and viral RNA helicase NS3 that influence the induction of apoptosis in response to infection with dengue type 1 virus. Virology 2000; 274:292-308.
16. Tan CH, Yap EH, Singh M, Deubel V, and Chan YC: Passive protection studies in mice with monoclonal antibodies directed against the non-structural protein NS3 of dengue 1 virus. J Gen Virol 1990;71:745-749.
17. Katrak K, Mahon BP, Jones WC, Brautigam S, and Mills KH: Preparative separation of foreign antigens for highly efficient presentation to T cells in vitro. J Immunol Methods 1992;156: 247-254.
18. Kohler G, and Milstein C: Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol 1976;6:511-519.
19. Pugachev KV, Nomokonova NY, Dobrikova EY, and Wolf YI: Site-directed mutagenesis of the tick-borne encephalitis virus NS3 gene reveals the putative serine protease domain of the NS3 protein. FEBS Lett 1993;328:115-118.
20. Wengler G, and Wengler G: The NS 3 nonstructural protein of flaviviruses contains an RNA triphosphatase activity. Virology 1993;197:265-273.
21. Westaway EG, Mackenzie JM, and Khromykh AA: Kunjin RNA replication and applications of Kunjin replicons. Adv Virus Res 2003;59:99-140.