Probing the mechanism of rubredoxin thermal unfolding in the absence of salt bridges by temperature jump experiments

Biochemical and Biophysical Research Communications

Volumn 333, Issue 3, 2005, Pages 839-844

  Henriques, Bárbara J ^a   Saraiva, Lígia M ^a   Gomes, Cláudio M ^a  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

Author keywords

Iron sulphur; Kinetics; Protein structure and folding; Thermal denaturation; Thermophile

Indexed keywords

8 ANILINO 1 NAPHTHALENESULFONIC ACID; RUBREDOXIN; STRUCTURAL PROTEIN; TRYPTOPHAN;

ABSORPTION SPECTROSCOPY; ARTICLE; DENATURATION; HYDROPHOBICITY; METHANOCOCCUS JANNASCHII; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE; PROTON TRANSPORT; REGULATORY MECHANISM; TEMPERATURE SENSITIVITY;

FLUORESCENCE; HYDROGEN-ION CONCENTRATION; KINETICS; METHANOCOCCUS; PROTEIN CONFORMATION; PROTEIN DENATURATION; RUBREDOXINS; SALTS; TEMPERATURE;

METHANOCALDOCOCCUS JANNASCHII; PYROCOCCUS FURIOSUS;

EID: 20744443027     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2005.06.004     Document Type: Article

References (15)

1. J. Meyer, J.M. Moulis, Rubredoxin, in Handbook of Metalloproteins, in: A. Messerschmidt, R. Huber, T. Poulos, K. Wieghardt (Eds.), John Wiley, Chichester, 2001, pp. 504-517.
2. C.M. Gomes, G. Silva, S. Oliveira, J. LeGall, M.Y. Liu, A.V. Xavier, C. Rodrigues-Pousada, and M. Teixeira Studies on the redox centers of the terminal oxidase from Desulfovibrio gigas and evidence for its interaction with rubredoxin J. Biol. Chem. 272 1997 22502 22508
3. J. LeGall, B.C. Prickril, I. Moura, A.V. Xavier, J.J. Moura, and B.H. Huynh Isolation and characterization of rubrerythrin, a non-heme iron protein from Desulfovibrio vulgaris that contains rubredoxin centers and a hemerythrin-like binuclear iron cluster Biochemistry 27 1988 1636 1642
4. C.M. Gomes, A. Giuffre, E. Forte, J.B. Vicente, L.M. Saraiva, M. Brunori, and M. Teixeira,c A novel type of nitric-oxide reductase. Escherichia coli flavorubredoxin J. Biol. Chem. 277 2002 25273 25276
5. C.M. Gomes, J.B. Vicente, A. Wasserfallen, and M. Teixeira Spectroscopic studies and characterization of a novel electron-transfer chain from Escherichia coli involving a flavorubredoxin and its flavoprotein reductase partner Biochemistry 39 2000 16230 16237
6. F. Bonomi, M.K. Eidsness, S. Iametti, D.M. Kurtz Jr., S. Mazzini, and A. Morleo Contribution of the [FeII(SCys)4] site to the thermostability of rubredoxins J. Biol. Inorg. Chem. 9 2004 297 306
7. F. Bonomi, D. Fessas, S. Iametti, D.M. Kurtz Jr., and S. Mazzini Thermal stability of Clostridium pasteurianum rubredoxin: deconvoluting the contributions of the metal site and the protein Protein Sci. 9 2000 2413 2426
8. P. Strop, and S.L. Mayo Contribution of surface salt bridges to protein stability Biochemistry 39 2000 1251 1255
9. S. Cavagnero, D.A. Debe, Z.H. Zhou, M.W. Adams, and S.I. Chan Kinetic role of electrostatic interactions in the unfolding of hyperthermophilic and mesophilic rubredoxins Biochemistry 37 1998 3369 3376
10. S. Cavagnero, Z.H. Zhou, M.W. Adams, and S.I. Chan Unfolding mechanism of rubredoxin from Pyrococcus furiosus Biochemistry 37 1998 3377 3385
11. M.W. Day, B.T. Hsu, L. Joshua-Tor, J.B. Park, Z.H. Zhou, M.W. Adams, and D.C. Rees X-ray crystal structures of the oxidized and reduced forms of the rubredoxin from the marine hyperthermophilic archaebacterium Pyrococcus furiosus Protein Sci. 1 1992 1494 1507
12. P.R. Blake, J.B. Park, F.O. Bryant, S. Aono, J.K. Magnuson, E. Eccleston, J.B. Howard, M.F. Summers, and M.W. Adams Determinants of protein hyperthermostability: purification and amino acid sequence of rubredoxin from the hyperthermophilic archaebacterium Pyrococcus furiosus and secondary structure of the zinc adduct by NMR Biochemistry 30 1991 10885 10895
13. J. LeGall, M.Y. Liu, C.M. Gomes, V. Braga, I. Pacheco, M. Regalla, A.V. Xavier, and M. Teixeira Characterisation of a new rubredoxin isolated from Desulfovibrio desulfuricans 27774: definition of a new family of rubredoxins FEBS Lett. 429 1998 295 298
14. C.A. Royer Fluorescence spectroscopy B.A. Shirley Methods in Molecular Biology 1995 Humana Press Totowa, New Jersey 377
15. G.V. Semisotnov, N.A. Rodionova, O.I. Razgulyaev, V.N. Uversky, A.F. Gripas, and R.I. Gilmanshin Study of the "molten globule" intermediate state in protein folding by a hydrophobic fluorescent probe Biopolymers 31 1991 119 128